Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07765 and MMJJ_RS07770 are separated by 71 nucleotides MMJJ_RS07770 and MMJJ_RS07775 are separated by 100 nucleotides MMJJ_RS07775 and MMJJ_RS07780 are separated by 4 nucleotides MMJJ_RS07780 and MMJJ_RS07785 are separated by 22 nucleotides
MMJJ_RS07765: MMJJ_RS07765 - DMT family protein, at 1,423,963 to 1,424,301
_RS07765
MMJJ_RS07770: MMJJ_RS07770 - tRNA-Arg, at 1,424,373 to 1,424,447
_RS07770
MMJJ_RS07775: MMJJ_RS07775 - fluoride efflux transporter CrcB, at 1,424,548 to 1,424,916
_RS07775
MMJJ_RS07780: MMJJ_RS07780 - DUF190 domain-containing protein, at 1,424,921 to 1,425,250
_RS07780
MMJJ_RS07785: MMJJ_RS07785 - fumarate reductase subunit A, at 1,425,273 to 1,426,892
_RS07785
Position (kb)
1424
1425
1426 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1423.925 kb on - strand at 1423.926 kb on + strand at 1423.926 kb on + strand at 1423.931 kb on - strand at 1423.931 kb on - strand at 1423.931 kb on - strand at 1423.932 kb on + strand at 1423.932 kb on + strand at 1423.932 kb on + strand at 1423.932 kb on + strand at 1423.932 kb on + strand at 1423.932 kb on + strand at 1423.932 kb on + strand at 1423.932 kb on + strand at 1423.934 kb on + strand at 1423.934 kb on + strand at 1423.935 kb on - strand at 1423.935 kb on - strand at 1423.939 kb on - strand at 1423.973 kb on + strand at 1424.004 kb on - strand, within MMJJ_RS07765 at 1424.036 kb on - strand, within MMJJ_RS07765 at 1424.037 kb on + strand, within MMJJ_RS07765 at 1424.044 kb on + strand, within MMJJ_RS07765 at 1424.140 kb on - strand, within MMJJ_RS07765 at 1424.145 kb on - strand, within MMJJ_RS07765 at 1424.146 kb on + strand, within MMJJ_RS07765 at 1424.146 kb on + strand, within MMJJ_RS07765 at 1424.160 kb on + strand, within MMJJ_RS07765 at 1424.166 kb on + strand, within MMJJ_RS07765 at 1424.316 kb on - strand at 1424.356 kb on - strand at 1424.473 kb on + strand at 1424.494 kb on - strand at 1424.496 kb on - strand at 1424.497 kb on + strand at 1424.497 kb on + strand at 1424.503 kb on - strand at 1424.525 kb on - strand at 1424.525 kb on - strand at 1424.525 kb on - strand at 1424.525 kb on - strand at 1424.529 kb on + strand at 1424.529 kb on + strand at 1424.534 kb on - strand at 1424.562 kb on + strand at 1424.595 kb on + strand, within MMJJ_RS07775 at 1424.597 kb on - strand, within MMJJ_RS07775 at 1424.602 kb on - strand, within MMJJ_RS07775 at 1424.603 kb on + strand, within MMJJ_RS07775 at 1424.649 kb on - strand, within MMJJ_RS07775 at 1424.649 kb on - strand, within MMJJ_RS07775 at 1424.650 kb on + strand, within MMJJ_RS07775 at 1424.656 kb on + strand, within MMJJ_RS07775 at 1424.693 kb on - strand, within MMJJ_RS07775 at 1424.694 kb on + strand, within MMJJ_RS07775 at 1424.696 kb on - strand, within MMJJ_RS07775 at 1424.698 kb on - strand at 1424.699 kb on + strand, within MMJJ_RS07775 at 1424.699 kb on + strand, within MMJJ_RS07775 at 1424.699 kb on + strand, within MMJJ_RS07775 at 1424.709 kb on + strand, within MMJJ_RS07775 at 1424.772 kb on - strand, within MMJJ_RS07775 at 1424.792 kb on + strand, within MMJJ_RS07775 at 1424.802 kb on - strand, within MMJJ_RS07775 at 1424.811 kb on + strand, within MMJJ_RS07775 at 1424.821 kb on - strand, within MMJJ_RS07775 at 1424.880 kb on + strand at 1424.972 kb on + strand, within MMJJ_RS07780 at 1424.972 kb on + strand, within MMJJ_RS07780 at 1424.972 kb on + strand, within MMJJ_RS07780 at 1424.988 kb on - strand, within MMJJ_RS07780 at 1424.989 kb on + strand, within MMJJ_RS07780 at 1425.086 kb on + strand, within MMJJ_RS07780 at 1425.094 kb on - strand, within MMJJ_RS07780 at 1425.096 kb on - strand, within MMJJ_RS07780 at 1425.097 kb on + strand, within MMJJ_RS07780 at 1425.164 kb on + strand, within MMJJ_RS07780 at 1425.165 kb on - strand, within MMJJ_RS07780 at 1425.166 kb on + strand, within MMJJ_RS07780 at 1425.166 kb on + strand, within MMJJ_RS07780 at 1425.166 kb on + strand, within MMJJ_RS07780 at 1425.205 kb on - strand, within MMJJ_RS07780 at 1425.205 kb on - strand, within MMJJ_RS07780 at 1425.205 kb on - strand, within MMJJ_RS07780 at 1425.206 kb on + strand, within MMJJ_RS07780 at 1425.206 kb on + strand, within MMJJ_RS07780 at 1425.207 kb on - strand, within MMJJ_RS07780 at 1425.207 kb on - strand, within MMJJ_RS07780 at 1425.229 kb on + strand at 1425.229 kb on + strand at 1425.229 kb on + strand at 1425.287 kb on + strand at 1425.352 kb on - strand at 1426.056 kb on + strand, within MMJJ_RS07785 at 1426.130 kb on + strand, within MMJJ_RS07785
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,423,925 - -1.8 1,423,926 + +1.9 1,423,926 + +0.9 1,423,931 - +0.8 1,423,931 - +0.9 1,423,931 - +1.5 1,423,932 + -0.4 1,423,932 + -0.1 1,423,932 + +1.8 1,423,932 + -0.8 1,423,932 + -0.5 1,423,932 + -0.9 1,423,932 + +0.7 1,423,932 + -0.3 1,423,934 + +0.8 1,423,934 + +1.0 1,423,935 - +0.8 1,423,935 - +0.6 1,423,939 - +0.3 1,423,973 + +0.1 1,424,004 - MMJJ_RS07765 0.12 +0.9 1,424,036 - MMJJ_RS07765 0.22 +1.7 1,424,037 + MMJJ_RS07765 0.22 -0.0 1,424,044 + MMJJ_RS07765 0.24 -0.3 1,424,140 - MMJJ_RS07765 0.52 +0.9 1,424,145 - MMJJ_RS07765 0.54 -0.3 1,424,146 + MMJJ_RS07765 0.54 +0.7 1,424,146 + MMJJ_RS07765 0.54 -1.1 1,424,160 + MMJJ_RS07765 0.58 -1.2 1,424,166 + MMJJ_RS07765 0.60 -1.4 1,424,316 - -0.8 1,424,356 - -0.6 1,424,473 + -2.2 1,424,494 - +0.4 1,424,496 - -1.9 1,424,497 + -0.9 1,424,497 + -0.6 1,424,503 - -1.4 1,424,525 - +0.3 1,424,525 - -2.3 1,424,525 - +0.7 1,424,525 - -0.6 1,424,529 + -0.4 1,424,529 + -0.4 1,424,534 - +0.7 1,424,562 + +0.1 1,424,595 + MMJJ_RS07775 0.13 +0.6 1,424,597 - MMJJ_RS07775 0.13 +0.9 1,424,602 - MMJJ_RS07775 0.15 -2.7 1,424,603 + MMJJ_RS07775 0.15 -0.3 1,424,649 - MMJJ_RS07775 0.27 -0.3 1,424,649 - MMJJ_RS07775 0.27 -0.5 1,424,650 + MMJJ_RS07775 0.28 -0.2 1,424,656 + MMJJ_RS07775 0.29 -2.9 1,424,693 - MMJJ_RS07775 0.39 -0.9 1,424,694 + MMJJ_RS07775 0.40 -0.6 1,424,696 - MMJJ_RS07775 0.40 -1.6 1,424,698 - -0.6 1,424,699 + MMJJ_RS07775 0.41 -0.9 1,424,699 + MMJJ_RS07775 0.41 +1.3 1,424,699 + MMJJ_RS07775 0.41 +1.6 1,424,709 + MMJJ_RS07775 0.44 -1.6 1,424,772 - MMJJ_RS07775 0.61 +0.8 1,424,792 + MMJJ_RS07775 0.66 -2.1 1,424,802 - MMJJ_RS07775 0.69 -1.6 1,424,811 + MMJJ_RS07775 0.71 +0.1 1,424,821 - MMJJ_RS07775 0.74 -0.0 1,424,880 + +1.6 1,424,972 + MMJJ_RS07780 0.15 -1.4 1,424,972 + MMJJ_RS07780 0.15 +1.5 1,424,972 + MMJJ_RS07780 0.15 +0.1 1,424,988 - MMJJ_RS07780 0.20 -1.6 1,424,989 + MMJJ_RS07780 0.21 -0.8 1,425,086 + MMJJ_RS07780 0.50 +1.5 1,425,094 - MMJJ_RS07780 0.52 -0.2 1,425,096 - MMJJ_RS07780 0.53 -2.5 1,425,097 + MMJJ_RS07780 0.53 +1.0 1,425,164 + MMJJ_RS07780 0.74 +0.6 1,425,165 - MMJJ_RS07780 0.74 -0.1 1,425,166 + MMJJ_RS07780 0.74 +0.5 1,425,166 + MMJJ_RS07780 0.74 +1.6 1,425,166 + MMJJ_RS07780 0.74 -0.2 1,425,205 - MMJJ_RS07780 0.86 +0.5 1,425,205 - MMJJ_RS07780 0.86 +1.0 1,425,205 - MMJJ_RS07780 0.86 +0.7 1,425,206 + MMJJ_RS07780 0.86 +0.7 1,425,206 + MMJJ_RS07780 0.86 +0.7 1,425,207 - MMJJ_RS07780 0.87 -0.8 1,425,207 - MMJJ_RS07780 0.87 +1.6 1,425,229 + +0.4 1,425,229 + -1.0 1,425,229 + -0.4 1,425,287 + -1.8 1,425,352 - -1.2 1,426,056 + MMJJ_RS07785 0.48 -1.4 1,426,130 + MMJJ_RS07785 0.53 -2.6
Or see this region's nucleotide sequence