Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06780 and MMJJ_RS06785 are separated by 54 nucleotides MMJJ_RS06785 and MMJJ_RS06790 are separated by 15 nucleotides MMJJ_RS06790 and MMJJ_RS06795 are separated by 101 nucleotides MMJJ_RS06795 and MMJJ_RS06800 are separated by 126 nucleotides MMJJ_RS06800 and MMJJ_RS06805 are separated by 194 nucleotides
MMJJ_RS06780: MMJJ_RS06780 - DUF356 domain-containing protein, at 1,254,380 to 1,254,760
_RS06780
MMJJ_RS06785: MMJJ_RS06785 - RNA ligase partner protein, at 1,254,815 to 1,255,486
_RS06785
MMJJ_RS06790: MMJJ_RS06790 - nucleotidyltransferase family protein, at 1,255,502 to 1,256,551
_RS06790
MMJJ_RS06795: MMJJ_RS06795 - tRNA-Val, at 1,256,653 to 1,256,729
_RS06795
MMJJ_RS06800: MMJJ_RS06800 - prefoldin subunit alpha, at 1,256,856 to 1,257,290
_RS06800
MMJJ_RS06805: MMJJ_RS06805 - monovalent cation/H+ antiporter subunit E, at 1,257,485 to 1,257,787
_RS06805
Position (kb) 1255
1256
1257 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 1254.506 kb on - strand, within MMJJ_RS06780 at 1254.799 kb on - strand at 1254.823 kb on - strand at 1254.851 kb on - strand at 1254.851 kb on - strand at 1254.971 kb on - strand, within MMJJ_RS06785 at 1255.036 kb on - strand, within MMJJ_RS06785 at 1255.075 kb on - strand, within MMJJ_RS06785 at 1255.075 kb on - strand, within MMJJ_RS06785 at 1255.104 kb on - strand, within MMJJ_RS06785 at 1255.104 kb on - strand, within MMJJ_RS06785 at 1255.104 kb on - strand, within MMJJ_RS06785 at 1255.104 kb on - strand, within MMJJ_RS06785 at 1255.104 kb on - strand, within MMJJ_RS06785 at 1255.126 kb on + strand, within MMJJ_RS06785 at 1255.142 kb on - strand, within MMJJ_RS06785 at 1255.157 kb on + strand, within MMJJ_RS06785 at 1255.181 kb on - strand, within MMJJ_RS06785 at 1255.185 kb on - strand, within MMJJ_RS06785 at 1255.186 kb on + strand, within MMJJ_RS06785 at 1255.186 kb on + strand, within MMJJ_RS06785 at 1255.186 kb on + strand, within MMJJ_RS06785 at 1255.186 kb on + strand, within MMJJ_RS06785 at 1255.191 kb on - strand, within MMJJ_RS06785 at 1255.191 kb on - strand, within MMJJ_RS06785 at 1255.196 kb on - strand, within MMJJ_RS06785 at 1255.196 kb on - strand, within MMJJ_RS06785 at 1255.196 kb on - strand, within MMJJ_RS06785 at 1255.215 kb on + strand, within MMJJ_RS06785 at 1255.222 kb on + strand, within MMJJ_RS06785 at 1255.315 kb on - strand, within MMJJ_RS06785 at 1255.315 kb on - strand, within MMJJ_RS06785 at 1255.386 kb on + strand, within MMJJ_RS06785 at 1255.447 kb on - strand at 1255.448 kb on + strand at 1255.460 kb on + strand at 1255.513 kb on - strand at 1255.514 kb on + strand at 1255.514 kb on + strand at 1255.517 kb on - strand at 1255.560 kb on - strand at 1255.570 kb on - strand at 1255.570 kb on - strand at 1255.571 kb on + strand at 1255.571 kb on + strand at 1255.584 kb on + strand at 1255.646 kb on + strand, within MMJJ_RS06790 at 1255.646 kb on + strand, within MMJJ_RS06790 at 1255.656 kb on + strand, within MMJJ_RS06790 at 1255.675 kb on + strand, within MMJJ_RS06790 at 1255.675 kb on + strand, within MMJJ_RS06790 at 1255.677 kb on - strand, within MMJJ_RS06790 at 1255.678 kb on + strand, within MMJJ_RS06790 at 1255.719 kb on - strand, within MMJJ_RS06790 at 1255.720 kb on + strand, within MMJJ_RS06790 at 1255.796 kb on + strand, within MMJJ_RS06790 at 1255.898 kb on - strand, within MMJJ_RS06790 at 1255.899 kb on + strand, within MMJJ_RS06790 at 1255.901 kb on + strand, within MMJJ_RS06790 at 1255.995 kb on - strand, within MMJJ_RS06790 at 1255.995 kb on - strand, within MMJJ_RS06790 at 1255.995 kb on - strand, within MMJJ_RS06790 at 1255.995 kb on - strand, within MMJJ_RS06790 at 1255.995 kb on - strand, within MMJJ_RS06790 at 1255.995 kb on - strand, within MMJJ_RS06790 at 1255.995 kb on - strand, within MMJJ_RS06790 at 1255.995 kb on - strand, within MMJJ_RS06790 at 1255.996 kb on + strand, within MMJJ_RS06790 at 1255.996 kb on + strand, within MMJJ_RS06790 at 1255.996 kb on + strand, within MMJJ_RS06790 at 1255.996 kb on + strand, within MMJJ_RS06790 at 1255.996 kb on + strand, within MMJJ_RS06790 at 1255.996 kb on + strand, within MMJJ_RS06790 at 1255.996 kb on + strand, within MMJJ_RS06790 at 1255.996 kb on + strand, within MMJJ_RS06790 at 1256.053 kb on - strand, within MMJJ_RS06790 at 1256.053 kb on - strand, within MMJJ_RS06790 at 1256.053 kb on - strand, within MMJJ_RS06790 at 1256.053 kb on - strand, within MMJJ_RS06790 at 1256.053 kb on - strand, within MMJJ_RS06790 at 1256.053 kb on - strand, within MMJJ_RS06790 at 1256.053 kb on - strand, within MMJJ_RS06790 at 1256.053 kb on - strand, within MMJJ_RS06790 at 1256.054 kb on + strand, within MMJJ_RS06790 at 1256.054 kb on + strand, within MMJJ_RS06790 at 1256.054 kb on + strand, within MMJJ_RS06790 at 1256.054 kb on + strand, within MMJJ_RS06790 at 1256.054 kb on + strand, within MMJJ_RS06790 at 1256.087 kb on - strand, within MMJJ_RS06790 at 1256.095 kb on - strand, within MMJJ_RS06790 at 1256.125 kb on - strand, within MMJJ_RS06790 at 1256.126 kb on + strand, within MMJJ_RS06790 at 1256.140 kb on - strand, within MMJJ_RS06790 at 1256.140 kb on - strand, within MMJJ_RS06790 at 1256.141 kb on + strand, within MMJJ_RS06790 at 1256.143 kb on - strand at 1256.154 kb on + strand, within MMJJ_RS06790 at 1256.154 kb on + strand, within MMJJ_RS06790 at 1256.188 kb on + strand, within MMJJ_RS06790 at 1256.203 kb on - strand, within MMJJ_RS06790 at 1256.228 kb on - strand, within MMJJ_RS06790 at 1256.229 kb on + strand, within MMJJ_RS06790 at 1256.231 kb on - strand, within MMJJ_RS06790 at 1256.283 kb on + strand, within MMJJ_RS06790 at 1256.283 kb on + strand, within MMJJ_RS06790 at 1256.283 kb on + strand, within MMJJ_RS06790 at 1256.283 kb on + strand, within MMJJ_RS06790 at 1256.287 kb on + strand, within MMJJ_RS06790 at 1256.287 kb on + strand, within MMJJ_RS06790 at 1256.327 kb on + strand, within MMJJ_RS06790 at 1256.333 kb on - strand, within MMJJ_RS06790 at 1256.333 kb on - strand, within MMJJ_RS06790 at 1256.333 kb on - strand, within MMJJ_RS06790 at 1256.334 kb on + strand, within MMJJ_RS06790 at 1256.334 kb on + strand, within MMJJ_RS06790 at 1256.334 kb on + strand, within MMJJ_RS06790 at 1256.334 kb on + strand, within MMJJ_RS06790 at 1256.334 kb on + strand, within MMJJ_RS06790 at 1256.334 kb on + strand, within MMJJ_RS06790 at 1256.334 kb on + strand, within MMJJ_RS06790 at 1256.335 kb on - strand, within MMJJ_RS06790 at 1256.335 kb on - strand, within MMJJ_RS06790 at 1256.336 kb on + strand, within MMJJ_RS06790 at 1256.370 kb on - strand, within MMJJ_RS06790 at 1256.371 kb on + strand, within MMJJ_RS06790 at 1256.371 kb on + strand, within MMJJ_RS06790 at 1256.411 kb on - strand, within MMJJ_RS06790 at 1256.412 kb on + strand, within MMJJ_RS06790 at 1256.412 kb on + strand, within MMJJ_RS06790 at 1256.508 kb on + strand at 1256.740 kb on + strand at 1256.799 kb on - strand at 1256.799 kb on - strand at 1256.800 kb on + strand at 1256.800 kb on + strand at 1256.804 kb on - strand at 1256.810 kb on + strand at 1256.812 kb on - strand at 1256.812 kb on - strand at 1256.813 kb on + strand at 1256.813 kb on + strand at 1256.813 kb on + strand at 1256.816 kb on - strand at 1256.816 kb on - strand at 1256.817 kb on + strand at 1256.817 kb on + strand at 1256.817 kb on + strand at 1256.817 kb on + strand at 1256.817 kb on + strand at 1256.858 kb on + strand at 1256.867 kb on - strand at 1257.003 kb on + strand, within MMJJ_RS06800 at 1257.018 kb on - strand, within MMJJ_RS06800 at 1257.019 kb on + strand, within MMJJ_RS06800 at 1257.024 kb on - strand, within MMJJ_RS06800 at 1257.024 kb on - strand, within MMJJ_RS06800 at 1257.025 kb on + strand, within MMJJ_RS06800 at 1257.025 kb on + strand, within MMJJ_RS06800 at 1257.025 kb on + strand, within MMJJ_RS06800 at 1257.033 kb on - strand, within MMJJ_RS06800 at 1257.033 kb on - strand, within MMJJ_RS06800 at 1257.034 kb on + strand, within MMJJ_RS06800 at 1257.034 kb on + strand, within MMJJ_RS06800 at 1257.049 kb on + strand, within MMJJ_RS06800 at 1257.049 kb on + strand, within MMJJ_RS06800 at 1257.200 kb on - strand, within MMJJ_RS06800 at 1257.201 kb on + strand, within MMJJ_RS06800 at 1257.201 kb on + strand, within MMJJ_RS06800 at 1257.201 kb on + strand, within MMJJ_RS06800 at 1257.303 kb on - strand at 1257.304 kb on + strand at 1257.304 kb on + strand at 1257.306 kb on - strand at 1257.306 kb on - strand at 1257.307 kb on + strand at 1257.307 kb on + strand at 1257.327 kb on + strand at 1257.336 kb on - strand at 1257.337 kb on + strand at 1257.337 kb on + strand at 1257.349 kb on - strand at 1257.375 kb on - strand at 1257.376 kb on + strand at 1257.376 kb on + strand at 1257.383 kb on - strand at 1257.384 kb on + strand at 1257.390 kb on - strand at 1257.420 kb on - strand at 1257.420 kb on - strand at 1257.420 kb on - strand at 1257.421 kb on + strand at 1257.421 kb on + strand at 1257.421 kb on + strand at 1257.421 kb on + strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.422 kb on - strand at 1257.423 kb on + strand at 1257.423 kb on + strand at 1257.423 kb on + strand at 1257.423 kb on + strand at 1257.423 kb on + strand at 1257.423 kb on + strand at 1257.423 kb on + strand at 1257.425 kb on + strand at 1257.425 kb on + strand at 1257.425 kb on + strand at 1257.425 kb on + strand at 1257.425 kb on + strand at 1257.425 kb on + strand at 1257.427 kb on + strand at 1257.427 kb on + strand at 1257.430 kb on - strand at 1257.483 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,254,506 - MMJJ_RS06780 0.33 +0.8 1,254,799 - +0.1 1,254,823 - -0.6 1,254,851 - -2.9 1,254,851 - -0.3 1,254,971 - MMJJ_RS06785 0.23 -3.6 1,255,036 - MMJJ_RS06785 0.33 -2.6 1,255,075 - MMJJ_RS06785 0.39 -4.0 1,255,075 - MMJJ_RS06785 0.39 -3.8 1,255,104 - MMJJ_RS06785 0.43 -2.9 1,255,104 - MMJJ_RS06785 0.43 +0.2 1,255,104 - MMJJ_RS06785 0.43 -3.2 1,255,104 - MMJJ_RS06785 0.43 -3.0 1,255,104 - MMJJ_RS06785 0.43 -3.0 1,255,126 + MMJJ_RS06785 0.46 -1.0 1,255,142 - MMJJ_RS06785 0.49 -0.0 1,255,157 + MMJJ_RS06785 0.51 -1.6 1,255,181 - MMJJ_RS06785 0.54 -3.0 1,255,185 - MMJJ_RS06785 0.55 -0.8 1,255,186 + MMJJ_RS06785 0.55 -2.1 1,255,186 + MMJJ_RS06785 0.55 -4.4 1,255,186 + MMJJ_RS06785 0.55 -0.6 1,255,186 + MMJJ_RS06785 0.55 -1.3 1,255,191 - MMJJ_RS06785 0.56 -1.6 1,255,191 - MMJJ_RS06785 0.56 -3.0 1,255,196 - MMJJ_RS06785 0.57 -3.0 1,255,196 - MMJJ_RS06785 0.57 -2.0 1,255,196 - MMJJ_RS06785 0.57 -3.6 1,255,215 + MMJJ_RS06785 0.60 -1.0 1,255,222 + MMJJ_RS06785 0.61 -1.4 1,255,315 - MMJJ_RS06785 0.74 -5.0 1,255,315 - MMJJ_RS06785 0.74 -2.8 1,255,386 + MMJJ_RS06785 0.85 -1.6 1,255,447 - -1.2 1,255,448 + -2.8 1,255,460 + -1.8 1,255,513 - +1.9 1,255,514 + -0.5 1,255,514 + -1.6 1,255,517 - -0.3 1,255,560 - -0.6 1,255,570 - -0.7 1,255,570 - +0.1 1,255,571 + -0.0 1,255,571 + -1.5 1,255,584 + -0.2 1,255,646 + MMJJ_RS06790 0.14 -0.6 1,255,646 + MMJJ_RS06790 0.14 +0.4 1,255,656 + MMJJ_RS06790 0.15 -0.3 1,255,675 + MMJJ_RS06790 0.16 +1.3 1,255,675 + MMJJ_RS06790 0.16 -0.2 1,255,677 - MMJJ_RS06790 0.17 -0.7 1,255,678 + MMJJ_RS06790 0.17 -0.0 1,255,719 - MMJJ_RS06790 0.21 +0.8 1,255,720 + MMJJ_RS06790 0.21 -0.7 1,255,796 + MMJJ_RS06790 0.28 -1.0 1,255,898 - MMJJ_RS06790 0.38 -1.9 1,255,899 + MMJJ_RS06790 0.38 -0.5 1,255,901 + MMJJ_RS06790 0.38 +1.6 1,255,995 - MMJJ_RS06790 0.47 +1.0 1,255,995 - MMJJ_RS06790 0.47 +0.2 1,255,995 - MMJJ_RS06790 0.47 +0.8 1,255,995 - MMJJ_RS06790 0.47 -0.6 1,255,995 - MMJJ_RS06790 0.47 -0.8 1,255,995 - MMJJ_RS06790 0.47 +0.8 1,255,995 - MMJJ_RS06790 0.47 +0.8 1,255,995 - MMJJ_RS06790 0.47 +1.1 1,255,996 + MMJJ_RS06790 0.47 +0.3 1,255,996 + MMJJ_RS06790 0.47 -0.2 1,255,996 + MMJJ_RS06790 0.47 +0.7 1,255,996 + MMJJ_RS06790 0.47 +0.6 1,255,996 + MMJJ_RS06790 0.47 -0.1 1,255,996 + MMJJ_RS06790 0.47 -0.0 1,255,996 + MMJJ_RS06790 0.47 -1.5 1,255,996 + MMJJ_RS06790 0.47 +0.5 1,256,053 - MMJJ_RS06790 0.52 +0.4 1,256,053 - MMJJ_RS06790 0.52 -0.3 1,256,053 - MMJJ_RS06790 0.52 +0.2 1,256,053 - MMJJ_RS06790 0.52 -0.4 1,256,053 - MMJJ_RS06790 0.52 +0.0 1,256,053 - MMJJ_RS06790 0.52 +0.9 1,256,053 - MMJJ_RS06790 0.52 +1.0 1,256,053 - MMJJ_RS06790 0.52 +0.5 1,256,054 + MMJJ_RS06790 0.53 +1.1 1,256,054 + MMJJ_RS06790 0.53 +0.4 1,256,054 + MMJJ_RS06790 0.53 +1.0 1,256,054 + MMJJ_RS06790 0.53 +0.7 1,256,054 + MMJJ_RS06790 0.53 +0.4 1,256,087 - MMJJ_RS06790 0.56 -0.2 1,256,095 - MMJJ_RS06790 0.56 +0.7 1,256,125 - MMJJ_RS06790 0.59 +1.0 1,256,126 + MMJJ_RS06790 0.59 +1.5 1,256,140 - MMJJ_RS06790 0.61 -2.1 1,256,140 - MMJJ_RS06790 0.61 +1.0 1,256,141 + MMJJ_RS06790 0.61 +0.8 1,256,143 - -1.0 1,256,154 + MMJJ_RS06790 0.62 +0.2 1,256,154 + MMJJ_RS06790 0.62 +0.8 1,256,188 + MMJJ_RS06790 0.65 -0.0 1,256,203 - MMJJ_RS06790 0.67 -1.2 1,256,228 - MMJJ_RS06790 0.69 +0.4 1,256,229 + MMJJ_RS06790 0.69 -0.3 1,256,231 - MMJJ_RS06790 0.69 +0.5 1,256,283 + MMJJ_RS06790 0.74 -0.2 1,256,283 + MMJJ_RS06790 0.74 -0.5 1,256,283 + MMJJ_RS06790 0.74 +0.4 1,256,283 + MMJJ_RS06790 0.74 -1.1 1,256,287 + MMJJ_RS06790 0.75 -0.4 1,256,287 + MMJJ_RS06790 0.75 -2.3 1,256,327 + MMJJ_RS06790 0.79 +0.3 1,256,333 - MMJJ_RS06790 0.79 -0.9 1,256,333 - MMJJ_RS06790 0.79 -0.4 1,256,333 - MMJJ_RS06790 0.79 -1.4 1,256,334 + MMJJ_RS06790 0.79 +0.8 1,256,334 + MMJJ_RS06790 0.79 +0.8 1,256,334 + MMJJ_RS06790 0.79 +1.2 1,256,334 + MMJJ_RS06790 0.79 +0.7 1,256,334 + MMJJ_RS06790 0.79 +0.2 1,256,334 + MMJJ_RS06790 0.79 -1.8 1,256,334 + MMJJ_RS06790 0.79 +1.2 1,256,335 - MMJJ_RS06790 0.79 -0.0 1,256,335 - MMJJ_RS06790 0.79 +0.6 1,256,336 + MMJJ_RS06790 0.79 -0.1 1,256,370 - MMJJ_RS06790 0.83 -0.5 1,256,371 + MMJJ_RS06790 0.83 +1.3 1,256,371 + MMJJ_RS06790 0.83 +0.9 1,256,411 - MMJJ_RS06790 0.87 -0.0 1,256,412 + MMJJ_RS06790 0.87 +0.1 1,256,412 + MMJJ_RS06790 0.87 -0.3 1,256,508 + +0.2 1,256,740 + +0.7 1,256,799 - +0.6 1,256,799 - +2.0 1,256,800 + +0.7 1,256,800 + +0.8 1,256,804 - +1.5 1,256,810 + -0.4 1,256,812 - +0.9 1,256,812 - +0.8 1,256,813 + +0.5 1,256,813 + -0.2 1,256,813 + -0.0 1,256,816 - +1.4 1,256,816 - +0.7 1,256,817 + +1.1 1,256,817 + -0.8 1,256,817 + +1.0 1,256,817 + -0.2 1,256,817 + +1.1 1,256,858 + +2.0 1,256,867 - -1.8 1,257,003 + MMJJ_RS06800 0.34 +0.4 1,257,018 - MMJJ_RS06800 0.37 +0.9 1,257,019 + MMJJ_RS06800 0.37 +0.2 1,257,024 - MMJJ_RS06800 0.39 +1.0 1,257,024 - MMJJ_RS06800 0.39 +1.2 1,257,025 + MMJJ_RS06800 0.39 +1.4 1,257,025 + MMJJ_RS06800 0.39 -0.4 1,257,025 + MMJJ_RS06800 0.39 -0.2 1,257,033 - MMJJ_RS06800 0.41 +0.6 1,257,033 - MMJJ_RS06800 0.41 +0.5 1,257,034 + MMJJ_RS06800 0.41 +0.6 1,257,034 + MMJJ_RS06800 0.41 -0.3 1,257,049 + MMJJ_RS06800 0.44 +0.6 1,257,049 + MMJJ_RS06800 0.44 -0.3 1,257,200 - MMJJ_RS06800 0.79 -1.8 1,257,201 + MMJJ_RS06800 0.79 -0.6 1,257,201 + MMJJ_RS06800 0.79 +1.6 1,257,201 + MMJJ_RS06800 0.79 +0.9 1,257,303 - -0.5 1,257,304 + +0.4 1,257,304 + +0.7 1,257,306 - +0.6 1,257,306 - +0.4 1,257,307 + +0.7 1,257,307 + +0.2 1,257,327 + +0.8 1,257,336 - +1.3 1,257,337 + +0.6 1,257,337 + +2.3 1,257,349 - -0.6 1,257,375 - -0.0 1,257,376 + -1.4 1,257,376 + +0.2 1,257,383 - -0.3 1,257,384 + +1.0 1,257,390 - -0.2 1,257,420 - -1.4 1,257,420 - -0.4 1,257,420 - -2.3 1,257,421 + -2.9 1,257,421 + -1.2 1,257,421 + -0.8 1,257,421 + +0.0 1,257,422 - -0.8 1,257,422 - +0.1 1,257,422 - -1.8 1,257,422 - -2.1 1,257,422 - -2.2 1,257,422 - -2.6 1,257,422 - -2.3 1,257,422 - -2.4 1,257,422 - -1.2 1,257,422 - -2.3 1,257,422 - -1.1 1,257,423 + -0.2 1,257,423 + -0.3 1,257,423 + -0.3 1,257,423 + +0.4 1,257,423 + -1.3 1,257,423 + -2.2 1,257,423 + -2.1 1,257,425 + -3.0 1,257,425 + -2.4 1,257,425 + -0.7 1,257,425 + -0.0 1,257,425 + -2.1 1,257,425 + -2.3 1,257,427 + -0.6 1,257,427 + -0.4 1,257,430 - -2.2 1,257,483 + -2.4
Or see this region's nucleotide sequence