Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06510 and MMJJ_RS06515 are separated by 42 nucleotides MMJJ_RS06515 and MMJJ_RS06520 are separated by 51 nucleotides MMJJ_RS06520 and MMJJ_RS06525 are separated by 107 nucleotides MMJJ_RS06525 and MMJJ_RS06530 are separated by 17 nucleotides
MMJJ_RS06510: MMJJ_RS06510 - LL-diaminopimelate aminotransferase, at 1,200,426 to 1,201,676
_RS06510
MMJJ_RS06515: MMJJ_RS06515 - ribonuclease III, at 1,201,719 to 1,202,408
_RS06515
MMJJ_RS06520: MMJJ_RS06520 - TldD/PmbA family protein, at 1,202,460 to 1,203,722
_RS06520
MMJJ_RS06525: MMJJ_RS06525 - protein translocase subunit SecF, at 1,203,830 to 1,204,675
_RS06525
MMJJ_RS06530: MMJJ_RS06530 - preprotein translocase subunit SecD, at 1,204,693 to 1,205,859
_RS06530
Position (kb)
1202
1203
1204 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1201.681 kb on - strand at 1201.716 kb on + strand at 1201.743 kb on - strand at 1201.811 kb on - strand, within MMJJ_RS06515 at 1201.883 kb on + strand, within MMJJ_RS06515 at 1201.977 kb on - strand, within MMJJ_RS06515 at 1201.978 kb on + strand, within MMJJ_RS06515 at 1201.978 kb on + strand, within MMJJ_RS06515 at 1201.985 kb on - strand, within MMJJ_RS06515 at 1201.986 kb on + strand, within MMJJ_RS06515 at 1201.986 kb on + strand, within MMJJ_RS06515 at 1201.987 kb on - strand, within MMJJ_RS06515 at 1201.987 kb on - strand, within MMJJ_RS06515 at 1201.987 kb on - strand, within MMJJ_RS06515 at 1201.988 kb on + strand, within MMJJ_RS06515 at 1201.990 kb on + strand, within MMJJ_RS06515 at 1201.990 kb on + strand, within MMJJ_RS06515 at 1202.022 kb on - strand, within MMJJ_RS06515 at 1202.023 kb on + strand, within MMJJ_RS06515 at 1202.094 kb on - strand, within MMJJ_RS06515 at 1202.095 kb on + strand, within MMJJ_RS06515 at 1202.115 kb on - strand, within MMJJ_RS06515 at 1202.115 kb on - strand, within MMJJ_RS06515 at 1202.115 kb on - strand, within MMJJ_RS06515 at 1202.116 kb on + strand, within MMJJ_RS06515 at 1202.116 kb on + strand, within MMJJ_RS06515 at 1202.116 kb on + strand, within MMJJ_RS06515 at 1202.119 kb on - strand, within MMJJ_RS06515 at 1202.119 kb on - strand, within MMJJ_RS06515 at 1202.147 kb on + strand, within MMJJ_RS06515 at 1202.147 kb on + strand, within MMJJ_RS06515 at 1202.173 kb on - strand, within MMJJ_RS06515 at 1202.174 kb on + strand, within MMJJ_RS06515 at 1202.174 kb on + strand, within MMJJ_RS06515 at 1202.174 kb on + strand, within MMJJ_RS06515 at 1202.230 kb on - strand, within MMJJ_RS06515 at 1202.231 kb on + strand, within MMJJ_RS06515 at 1202.241 kb on - strand, within MMJJ_RS06515 at 1202.241 kb on - strand, within MMJJ_RS06515 at 1202.242 kb on + strand, within MMJJ_RS06515 at 1202.242 kb on + strand, within MMJJ_RS06515 at 1202.242 kb on + strand, within MMJJ_RS06515 at 1202.242 kb on + strand, within MMJJ_RS06515 at 1202.244 kb on - strand, within MMJJ_RS06515 at 1202.245 kb on + strand, within MMJJ_RS06515 at 1202.269 kb on - strand, within MMJJ_RS06515 at 1202.270 kb on + strand, within MMJJ_RS06515 at 1202.285 kb on - strand, within MMJJ_RS06515 at 1202.298 kb on + strand, within MMJJ_RS06515 at 1202.316 kb on - strand, within MMJJ_RS06515 at 1202.316 kb on - strand, within MMJJ_RS06515 at 1202.317 kb on + strand, within MMJJ_RS06515 at 1202.317 kb on + strand, within MMJJ_RS06515 at 1202.317 kb on + strand, within MMJJ_RS06515 at 1202.321 kb on + strand, within MMJJ_RS06515 at 1202.321 kb on + strand, within MMJJ_RS06515 at 1202.334 kb on + strand, within MMJJ_RS06515 at 1202.334 kb on + strand, within MMJJ_RS06515 at 1202.352 kb on + strand at 1202.355 kb on - strand at 1202.355 kb on - strand at 1202.364 kb on + strand at 1202.370 kb on - strand at 1202.370 kb on - strand at 1202.370 kb on - strand at 1202.371 kb on + strand at 1202.371 kb on + strand at 1202.371 kb on + strand at 1202.426 kb on - strand at 1202.440 kb on + strand at 1202.443 kb on - strand at 1202.443 kb on - strand at 1202.525 kb on - strand at 1202.547 kb on - strand at 1202.561 kb on - strand at 1202.561 kb on - strand at 1202.617 kb on - strand, within MMJJ_RS06520 at 1202.651 kb on - strand, within MMJJ_RS06520 at 1202.652 kb on + strand, within MMJJ_RS06520 at 1202.666 kb on - strand, within MMJJ_RS06520 at 1202.667 kb on + strand, within MMJJ_RS06520 at 1202.679 kb on - strand, within MMJJ_RS06520 at 1202.680 kb on + strand, within MMJJ_RS06520 at 1202.693 kb on + strand, within MMJJ_RS06520 at 1202.693 kb on + strand, within MMJJ_RS06520 at 1202.698 kb on - strand, within MMJJ_RS06520 at 1202.699 kb on + strand, within MMJJ_RS06520 at 1202.736 kb on - strand, within MMJJ_RS06520 at 1202.736 kb on - strand, within MMJJ_RS06520 at 1202.742 kb on + strand, within MMJJ_RS06520 at 1202.742 kb on + strand, within MMJJ_RS06520 at 1202.814 kb on + strand, within MMJJ_RS06520 at 1202.828 kb on - strand, within MMJJ_RS06520 at 1202.839 kb on - strand, within MMJJ_RS06520 at 1202.840 kb on + strand, within MMJJ_RS06520 at 1202.865 kb on + strand, within MMJJ_RS06520 at 1202.901 kb on - strand, within MMJJ_RS06520 at 1202.967 kb on - strand, within MMJJ_RS06520 at 1202.967 kb on - strand, within MMJJ_RS06520 at 1202.968 kb on + strand, within MMJJ_RS06520 at 1203.026 kb on - strand, within MMJJ_RS06520 at 1203.027 kb on + strand, within MMJJ_RS06520 at 1203.038 kb on - strand, within MMJJ_RS06520 at 1203.038 kb on - strand, within MMJJ_RS06520 at 1203.039 kb on + strand, within MMJJ_RS06520 at 1203.051 kb on - strand, within MMJJ_RS06520 at 1203.086 kb on - strand, within MMJJ_RS06520 at 1203.136 kb on + strand, within MMJJ_RS06520 at 1203.136 kb on + strand, within MMJJ_RS06520 at 1203.187 kb on - strand, within MMJJ_RS06520 at 1203.236 kb on + strand, within MMJJ_RS06520 at 1203.238 kb on - strand, within MMJJ_RS06520 at 1203.347 kb on - strand, within MMJJ_RS06520 at 1203.348 kb on + strand, within MMJJ_RS06520 at 1203.410 kb on + strand, within MMJJ_RS06520 at 1203.433 kb on - strand, within MMJJ_RS06520 at 1203.434 kb on + strand, within MMJJ_RS06520 at 1203.491 kb on - strand, within MMJJ_RS06520 at 1203.492 kb on + strand, within MMJJ_RS06520 at 1203.496 kb on + strand, within MMJJ_RS06520 at 1203.499 kb on + strand, within MMJJ_RS06520 at 1203.499 kb on + strand, within MMJJ_RS06520 at 1203.551 kb on - strand, within MMJJ_RS06520 at 1203.594 kb on + strand, within MMJJ_RS06520 at 1203.700 kb on + strand at 1203.703 kb on - strand at 1203.708 kb on - strand at 1203.726 kb on - strand at 1203.743 kb on - strand at 1203.745 kb on - strand at 1203.746 kb on + strand at 1203.746 kb on + strand at 1203.756 kb on - strand at 1203.758 kb on - strand at 1203.758 kb on - strand at 1203.759 kb on + strand at 1203.759 kb on + strand at 1203.765 kb on - strand at 1203.774 kb on + strand at 1204.249 kb on - strand, within MMJJ_RS06525 at 1204.527 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,201,681 - +0.8 1,201,716 + +0.1 1,201,743 - +0.1 1,201,811 - MMJJ_RS06515 0.13 -1.9 1,201,883 + MMJJ_RS06515 0.24 +1.8 1,201,977 - MMJJ_RS06515 0.37 +1.9 1,201,978 + MMJJ_RS06515 0.38 +0.5 1,201,978 + MMJJ_RS06515 0.38 +0.8 1,201,985 - MMJJ_RS06515 0.39 +1.4 1,201,986 + MMJJ_RS06515 0.39 -0.3 1,201,986 + MMJJ_RS06515 0.39 -3.0 1,201,987 - MMJJ_RS06515 0.39 +0.6 1,201,987 - MMJJ_RS06515 0.39 +0.5 1,201,987 - MMJJ_RS06515 0.39 -0.6 1,201,988 + MMJJ_RS06515 0.39 -0.3 1,201,990 + MMJJ_RS06515 0.39 +1.1 1,201,990 + MMJJ_RS06515 0.39 +1.3 1,202,022 - MMJJ_RS06515 0.44 +1.1 1,202,023 + MMJJ_RS06515 0.44 -1.6 1,202,094 - MMJJ_RS06515 0.54 +2.5 1,202,095 + MMJJ_RS06515 0.54 +2.4 1,202,115 - MMJJ_RS06515 0.57 +2.0 1,202,115 - MMJJ_RS06515 0.57 -0.5 1,202,115 - MMJJ_RS06515 0.57 -0.0 1,202,116 + MMJJ_RS06515 0.58 +0.5 1,202,116 + MMJJ_RS06515 0.58 -0.6 1,202,116 + MMJJ_RS06515 0.58 +1.1 1,202,119 - MMJJ_RS06515 0.58 -0.2 1,202,119 - MMJJ_RS06515 0.58 -0.0 1,202,147 + MMJJ_RS06515 0.62 -0.1 1,202,147 + MMJJ_RS06515 0.62 +1.3 1,202,173 - MMJJ_RS06515 0.66 +1.1 1,202,174 + MMJJ_RS06515 0.66 -0.2 1,202,174 + MMJJ_RS06515 0.66 +0.3 1,202,174 + MMJJ_RS06515 0.66 -0.5 1,202,230 - MMJJ_RS06515 0.74 -0.1 1,202,231 + MMJJ_RS06515 0.74 +0.7 1,202,241 - MMJJ_RS06515 0.76 +1.1 1,202,241 - MMJJ_RS06515 0.76 +0.1 1,202,242 + MMJJ_RS06515 0.76 -0.1 1,202,242 + MMJJ_RS06515 0.76 +1.1 1,202,242 + MMJJ_RS06515 0.76 +0.3 1,202,242 + MMJJ_RS06515 0.76 -0.7 1,202,244 - MMJJ_RS06515 0.76 +0.6 1,202,245 + MMJJ_RS06515 0.76 -0.0 1,202,269 - MMJJ_RS06515 0.80 +0.6 1,202,270 + MMJJ_RS06515 0.80 -1.4 1,202,285 - MMJJ_RS06515 0.82 +0.7 1,202,298 + MMJJ_RS06515 0.84 +0.7 1,202,316 - MMJJ_RS06515 0.87 +0.9 1,202,316 - MMJJ_RS06515 0.87 -0.2 1,202,317 + MMJJ_RS06515 0.87 +0.0 1,202,317 + MMJJ_RS06515 0.87 -0.5 1,202,317 + MMJJ_RS06515 0.87 -0.8 1,202,321 + MMJJ_RS06515 0.87 +1.3 1,202,321 + MMJJ_RS06515 0.87 -0.0 1,202,334 + MMJJ_RS06515 0.89 +1.2 1,202,334 + MMJJ_RS06515 0.89 +0.1 1,202,352 + +0.3 1,202,355 - +1.1 1,202,355 - +0.4 1,202,364 + +0.1 1,202,370 - -1.0 1,202,370 - +0.4 1,202,370 - -0.8 1,202,371 + -0.8 1,202,371 + +0.2 1,202,371 + +0.4 1,202,426 - -1.3 1,202,440 + +1.6 1,202,443 - -0.4 1,202,443 - -0.6 1,202,525 - +1.1 1,202,547 - +1.0 1,202,561 - -0.7 1,202,561 - +0.6 1,202,617 - MMJJ_RS06520 0.12 +0.7 1,202,651 - MMJJ_RS06520 0.15 -0.9 1,202,652 + MMJJ_RS06520 0.15 -0.3 1,202,666 - MMJJ_RS06520 0.16 +1.2 1,202,667 + MMJJ_RS06520 0.16 -2.3 1,202,679 - MMJJ_RS06520 0.17 -2.0 1,202,680 + MMJJ_RS06520 0.17 +1.1 1,202,693 + MMJJ_RS06520 0.18 +0.4 1,202,693 + MMJJ_RS06520 0.18 +0.1 1,202,698 - MMJJ_RS06520 0.19 +0.2 1,202,699 + MMJJ_RS06520 0.19 +0.1 1,202,736 - MMJJ_RS06520 0.22 +0.5 1,202,736 - MMJJ_RS06520 0.22 -0.4 1,202,742 + MMJJ_RS06520 0.22 +0.3 1,202,742 + MMJJ_RS06520 0.22 -0.0 1,202,814 + MMJJ_RS06520 0.28 -0.4 1,202,828 - MMJJ_RS06520 0.29 +0.4 1,202,839 - MMJJ_RS06520 0.30 -1.0 1,202,840 + MMJJ_RS06520 0.30 +0.0 1,202,865 + MMJJ_RS06520 0.32 +1.6 1,202,901 - MMJJ_RS06520 0.35 -0.5 1,202,967 - MMJJ_RS06520 0.40 +0.5 1,202,967 - MMJJ_RS06520 0.40 -0.1 1,202,968 + MMJJ_RS06520 0.40 -0.0 1,203,026 - MMJJ_RS06520 0.45 +0.6 1,203,027 + MMJJ_RS06520 0.45 +0.9 1,203,038 - MMJJ_RS06520 0.46 +0.1 1,203,038 - MMJJ_RS06520 0.46 +1.6 1,203,039 + MMJJ_RS06520 0.46 -0.6 1,203,051 - MMJJ_RS06520 0.47 +1.3 1,203,086 - MMJJ_RS06520 0.50 +0.7 1,203,136 + MMJJ_RS06520 0.54 +1.2 1,203,136 + MMJJ_RS06520 0.54 +2.0 1,203,187 - MMJJ_RS06520 0.58 +1.5 1,203,236 + MMJJ_RS06520 0.61 +0.8 1,203,238 - MMJJ_RS06520 0.62 -0.1 1,203,347 - MMJJ_RS06520 0.70 +1.3 1,203,348 + MMJJ_RS06520 0.70 -0.3 1,203,410 + MMJJ_RS06520 0.75 -1.5 1,203,433 - MMJJ_RS06520 0.77 +0.6 1,203,434 + MMJJ_RS06520 0.77 +1.3 1,203,491 - MMJJ_RS06520 0.82 -0.4 1,203,492 + MMJJ_RS06520 0.82 -0.4 1,203,496 + MMJJ_RS06520 0.82 +0.7 1,203,499 + MMJJ_RS06520 0.82 +1.8 1,203,499 + MMJJ_RS06520 0.82 +1.0 1,203,551 - MMJJ_RS06520 0.86 +0.6 1,203,594 + MMJJ_RS06520 0.90 +0.8 1,203,700 + +0.7 1,203,703 - -0.0 1,203,708 - -1.7 1,203,726 - -0.8 1,203,743 - -0.4 1,203,745 - -0.1 1,203,746 + +2.0 1,203,746 + +0.5 1,203,756 - +0.1 1,203,758 - -0.3 1,203,758 - +2.0 1,203,759 + -0.8 1,203,759 + -0.5 1,203,765 - +1.1 1,203,774 + -0.2 1,204,249 - MMJJ_RS06525 0.50 -1.0 1,204,527 - +0.6
Or see this region's nucleotide sequence