Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06285 and MMJJ_RS06290 are separated by 10 nucleotides MMJJ_RS06290 and MMJJ_RS06295 overlap by 23 nucleotides MMJJ_RS06295 and MMJJ_RS06300 are separated by 86 nucleotides
MMJJ_RS06285: MMJJ_RS06285 - YchF/TatD family DNA exonuclease, at 1,166,453 to 1,167,208
_RS06285
MMJJ_RS06290: MMJJ_RS06290 - DUF749 domain-containing protein, at 1,167,219 to 1,167,527
_RS06290
MMJJ_RS06295: MMJJ_RS06295 - DUF2096 family protein, at 1,167,505 to 1,168,038
_RS06295
MMJJ_RS06300: MMJJ_RS06300 - tetrahydromethanopterin S-methyltransferase subunit H, at 1,168,125 to 1,169,084
_RS06300
Position (kb)
1167
1168
1169 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1166.516 kb on + strand at 1166.516 kb on + strand at 1166.531 kb on + strand, within MMJJ_RS06285 at 1166.535 kb on - strand, within MMJJ_RS06285 at 1166.535 kb on - strand, within MMJJ_RS06285 at 1166.536 kb on + strand, within MMJJ_RS06285 at 1166.536 kb on + strand, within MMJJ_RS06285 at 1166.539 kb on - strand, within MMJJ_RS06285 at 1166.569 kb on + strand, within MMJJ_RS06285 at 1166.586 kb on - strand, within MMJJ_RS06285 at 1166.591 kb on + strand, within MMJJ_RS06285 at 1166.591 kb on + strand, within MMJJ_RS06285 at 1166.613 kb on - strand, within MMJJ_RS06285 at 1166.624 kb on - strand, within MMJJ_RS06285 at 1166.634 kb on + strand, within MMJJ_RS06285 at 1166.637 kb on - strand, within MMJJ_RS06285 at 1166.638 kb on + strand, within MMJJ_RS06285 at 1166.638 kb on + strand, within MMJJ_RS06285 at 1166.644 kb on + strand, within MMJJ_RS06285 at 1166.666 kb on + strand, within MMJJ_RS06285 at 1166.677 kb on - strand, within MMJJ_RS06285 at 1166.698 kb on - strand, within MMJJ_RS06285 at 1166.705 kb on + strand, within MMJJ_RS06285 at 1166.791 kb on + strand, within MMJJ_RS06285 at 1166.827 kb on - strand at 1166.970 kb on + strand, within MMJJ_RS06285 at 1166.974 kb on - strand, within MMJJ_RS06285 at 1166.975 kb on + strand, within MMJJ_RS06285 at 1166.982 kb on - strand, within MMJJ_RS06285 at 1166.982 kb on - strand, within MMJJ_RS06285 at 1167.022 kb on + strand, within MMJJ_RS06285 at 1167.052 kb on - strand, within MMJJ_RS06285 at 1167.052 kb on - strand, within MMJJ_RS06285 at 1167.052 kb on - strand, within MMJJ_RS06285 at 1167.146 kb on - strand at 1167.146 kb on - strand at 1167.176 kb on - strand at 1167.176 kb on - strand at 1167.176 kb on - strand at 1167.176 kb on - strand at 1167.177 kb on + strand at 1167.177 kb on + strand at 1167.195 kb on - strand at 1167.195 kb on - strand at 1167.195 kb on - strand at 1167.195 kb on - strand at 1167.196 kb on + strand at 1167.198 kb on + strand at 1167.198 kb on + strand at 1167.202 kb on - strand at 1167.215 kb on - strand at 1167.251 kb on - strand, within MMJJ_RS06290 at 1167.352 kb on - strand, within MMJJ_RS06290 at 1167.357 kb on + strand, within MMJJ_RS06290 at 1167.357 kb on + strand, within MMJJ_RS06290 at 1167.371 kb on - strand, within MMJJ_RS06290 at 1167.371 kb on - strand, within MMJJ_RS06290 at 1167.372 kb on + strand, within MMJJ_RS06290 at 1167.387 kb on - strand, within MMJJ_RS06290 at 1167.387 kb on - strand, within MMJJ_RS06290 at 1167.387 kb on - strand, within MMJJ_RS06290 at 1167.395 kb on + strand, within MMJJ_RS06290 at 1167.441 kb on - strand, within MMJJ_RS06290 at 1167.486 kb on - strand, within MMJJ_RS06290 at 1167.490 kb on - strand, within MMJJ_RS06290 at 1167.491 kb on + strand, within MMJJ_RS06290 at 1167.503 kb on - strand at 1167.539 kb on - strand at 1167.539 kb on - strand at 1167.542 kb on - strand at 1167.543 kb on + strand at 1167.543 kb on + strand at 1167.570 kb on - strand, within MMJJ_RS06295 at 1167.630 kb on - strand, within MMJJ_RS06295 at 1167.630 kb on - strand, within MMJJ_RS06295 at 1167.630 kb on - strand, within MMJJ_RS06295 at 1167.630 kb on - strand, within MMJJ_RS06295 at 1167.630 kb on - strand, within MMJJ_RS06295 at 1167.635 kb on + strand, within MMJJ_RS06295 at 1167.637 kb on - strand, within MMJJ_RS06295 at 1167.642 kb on + strand, within MMJJ_RS06295 at 1167.707 kb on - strand, within MMJJ_RS06295 at 1167.707 kb on - strand, within MMJJ_RS06295 at 1167.708 kb on + strand, within MMJJ_RS06295 at 1167.708 kb on + strand, within MMJJ_RS06295 at 1167.857 kb on - strand, within MMJJ_RS06295 at 1167.863 kb on - strand, within MMJJ_RS06295 at 1167.864 kb on + strand, within MMJJ_RS06295 at 1167.864 kb on + strand, within MMJJ_RS06295 at 1167.867 kb on - strand, within MMJJ_RS06295 at 1167.867 kb on - strand, within MMJJ_RS06295 at 1167.868 kb on + strand, within MMJJ_RS06295 at 1167.868 kb on + strand, within MMJJ_RS06295 at 1167.876 kb on + strand, within MMJJ_RS06295 at 1167.915 kb on - strand, within MMJJ_RS06295 at 1167.923 kb on - strand, within MMJJ_RS06295 at 1167.924 kb on + strand, within MMJJ_RS06295 at 1167.926 kb on - strand, within MMJJ_RS06295 at 1167.926 kb on - strand, within MMJJ_RS06295 at 1167.927 kb on + strand, within MMJJ_RS06295 at 1168.056 kb on + strand at 1168.056 kb on + strand at 1168.057 kb on - strand at 1168.057 kb on - strand at 1168.057 kb on - strand at 1168.057 kb on - strand at 1168.057 kb on - strand at 1168.058 kb on + strand at 1168.058 kb on + strand at 1168.123 kb on + strand at 1168.127 kb on + strand at 1168.127 kb on + strand at 1168.285 kb on + strand, within MMJJ_RS06300 at 1168.592 kb on - strand, within MMJJ_RS06300
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,166,516 + -0.5 1,166,516 + +2.4 1,166,531 + MMJJ_RS06285 0.10 +0.2 1,166,535 - MMJJ_RS06285 0.11 +0.2 1,166,535 - MMJJ_RS06285 0.11 +1.9 1,166,536 + MMJJ_RS06285 0.11 -0.1 1,166,536 + MMJJ_RS06285 0.11 +0.5 1,166,539 - MMJJ_RS06285 0.11 +1.0 1,166,569 + MMJJ_RS06285 0.15 +0.0 1,166,586 - MMJJ_RS06285 0.18 +0.3 1,166,591 + MMJJ_RS06285 0.18 -1.4 1,166,591 + MMJJ_RS06285 0.18 -0.2 1,166,613 - MMJJ_RS06285 0.21 +1.0 1,166,624 - MMJJ_RS06285 0.23 +0.0 1,166,634 + MMJJ_RS06285 0.24 -1.5 1,166,637 - MMJJ_RS06285 0.24 -1.6 1,166,638 + MMJJ_RS06285 0.24 -1.3 1,166,638 + MMJJ_RS06285 0.24 +2.4 1,166,644 + MMJJ_RS06285 0.25 +0.1 1,166,666 + MMJJ_RS06285 0.28 +1.0 1,166,677 - MMJJ_RS06285 0.30 -1.1 1,166,698 - MMJJ_RS06285 0.32 -0.7 1,166,705 + MMJJ_RS06285 0.33 -0.9 1,166,791 + MMJJ_RS06285 0.45 +1.3 1,166,827 - +0.4 1,166,970 + MMJJ_RS06285 0.68 +0.6 1,166,974 - MMJJ_RS06285 0.69 +0.9 1,166,975 + MMJJ_RS06285 0.69 -3.3 1,166,982 - MMJJ_RS06285 0.70 +0.5 1,166,982 - MMJJ_RS06285 0.70 +0.8 1,167,022 + MMJJ_RS06285 0.75 +1.1 1,167,052 - MMJJ_RS06285 0.79 -0.2 1,167,052 - MMJJ_RS06285 0.79 +0.4 1,167,052 - MMJJ_RS06285 0.79 -0.7 1,167,146 - -0.3 1,167,146 - +0.8 1,167,176 - -0.3 1,167,176 - -1.3 1,167,176 - -2.9 1,167,176 - +0.7 1,167,177 + -1.2 1,167,177 + -0.4 1,167,195 - +1.3 1,167,195 - -0.6 1,167,195 - -1.5 1,167,195 - +0.7 1,167,196 + -0.1 1,167,198 + +1.3 1,167,198 + +0.2 1,167,202 - +1.4 1,167,215 - +2.0 1,167,251 - MMJJ_RS06290 0.10 +0.7 1,167,352 - MMJJ_RS06290 0.43 -1.0 1,167,357 + MMJJ_RS06290 0.45 +0.2 1,167,357 + MMJJ_RS06290 0.45 +0.7 1,167,371 - MMJJ_RS06290 0.49 +2.0 1,167,371 - MMJJ_RS06290 0.49 -0.2 1,167,372 + MMJJ_RS06290 0.50 -0.6 1,167,387 - MMJJ_RS06290 0.54 +0.4 1,167,387 - MMJJ_RS06290 0.54 +0.6 1,167,387 - MMJJ_RS06290 0.54 -0.1 1,167,395 + MMJJ_RS06290 0.57 -0.6 1,167,441 - MMJJ_RS06290 0.72 -0.5 1,167,486 - MMJJ_RS06290 0.86 +1.0 1,167,490 - MMJJ_RS06290 0.88 -1.6 1,167,491 + MMJJ_RS06290 0.88 -0.2 1,167,503 - -0.4 1,167,539 - +1.3 1,167,539 - +0.4 1,167,542 - -0.1 1,167,543 + -3.8 1,167,543 + -1.2 1,167,570 - MMJJ_RS06295 0.12 +1.1 1,167,630 - MMJJ_RS06295 0.23 +0.6 1,167,630 - MMJJ_RS06295 0.23 -0.1 1,167,630 - MMJJ_RS06295 0.23 -0.9 1,167,630 - MMJJ_RS06295 0.23 +1.2 1,167,630 - MMJJ_RS06295 0.23 +1.1 1,167,635 + MMJJ_RS06295 0.24 +1.0 1,167,637 - MMJJ_RS06295 0.25 +0.6 1,167,642 + MMJJ_RS06295 0.26 -1.7 1,167,707 - MMJJ_RS06295 0.38 +0.7 1,167,707 - MMJJ_RS06295 0.38 -0.5 1,167,708 + MMJJ_RS06295 0.38 +0.8 1,167,708 + MMJJ_RS06295 0.38 -0.1 1,167,857 - MMJJ_RS06295 0.66 +0.3 1,167,863 - MMJJ_RS06295 0.67 +0.9 1,167,864 + MMJJ_RS06295 0.67 -0.3 1,167,864 + MMJJ_RS06295 0.67 -0.3 1,167,867 - MMJJ_RS06295 0.68 +1.0 1,167,867 - MMJJ_RS06295 0.68 -0.1 1,167,868 + MMJJ_RS06295 0.68 +1.7 1,167,868 + MMJJ_RS06295 0.68 +0.1 1,167,876 + MMJJ_RS06295 0.69 +0.1 1,167,915 - MMJJ_RS06295 0.77 +0.8 1,167,923 - MMJJ_RS06295 0.78 +0.7 1,167,924 + MMJJ_RS06295 0.78 -1.4 1,167,926 - MMJJ_RS06295 0.79 +1.2 1,167,926 - MMJJ_RS06295 0.79 +0.1 1,167,927 + MMJJ_RS06295 0.79 +1.0 1,168,056 + +0.6 1,168,056 + +1.0 1,168,057 - -1.1 1,168,057 - +1.6 1,168,057 - -1.7 1,168,057 - +0.2 1,168,057 - +1.0 1,168,058 + +0.6 1,168,058 + -0.6 1,168,123 + -0.7 1,168,127 + -0.3 1,168,127 + +1.3 1,168,285 + MMJJ_RS06300 0.17 -0.9 1,168,592 - MMJJ_RS06300 0.49 -2.8
Or see this region's nucleotide sequence