Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05395 and MMJJ_RS05400 are separated by 41 nucleotides MMJJ_RS05400 and MMJJ_RS05405 are separated by 69 nucleotides
MMJJ_RS05395: MMJJ_RS05395 - argininosuccinate lyase, at 1,001,596 to 1,003,041
_RS05395
MMJJ_RS05400: MMJJ_RS05400 - CoA-binding protein, at 1,003,083 to 1,003,532
_RS05400
MMJJ_RS05405: MMJJ_RS05405 - DNA cytosine methyltransferase, at 1,003,602 to 1,004,669
_RS05405
Position (kb)
1003
1004 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1002.092 kb on - strand, within MMJJ_RS05395 at 1003.071 kb on - strand at 1003.103 kb on - strand at 1003.103 kb on - strand at 1003.202 kb on - strand, within MMJJ_RS05400 at 1003.203 kb on + strand, within MMJJ_RS05400 at 1003.203 kb on + strand, within MMJJ_RS05400 at 1003.254 kb on - strand, within MMJJ_RS05400 at 1003.308 kb on - strand, within MMJJ_RS05400 at 1003.309 kb on + strand, within MMJJ_RS05400 at 1003.309 kb on + strand, within MMJJ_RS05400 at 1003.309 kb on + strand, within MMJJ_RS05400 at 1003.321 kb on + strand, within MMJJ_RS05400 at 1003.365 kb on + strand, within MMJJ_RS05400 at 1003.399 kb on - strand, within MMJJ_RS05400 at 1003.399 kb on - strand, within MMJJ_RS05400 at 1003.515 kb on + strand at 1003.534 kb on - strand at 1003.534 kb on - strand at 1003.534 kb on - strand at 1003.534 kb on - strand at 1003.534 kb on - strand at 1003.534 kb on - strand at 1003.535 kb on + strand at 1003.535 kb on + strand at 1003.545 kb on - strand at 1003.546 kb on + strand at 1003.546 kb on + strand at 1003.546 kb on + strand at 1003.553 kb on - strand at 1003.554 kb on + strand at 1003.554 kb on + strand at 1003.557 kb on - strand at 1003.557 kb on - strand at 1003.567 kb on - strand at 1003.568 kb on + strand at 1003.568 kb on + strand at 1003.568 kb on + strand at 1003.568 kb on + strand at 1003.568 kb on + strand at 1003.568 kb on + strand at 1003.569 kb on - strand at 1003.569 kb on - strand at 1003.569 kb on - strand at 1003.570 kb on + strand at 1003.570 kb on + strand at 1003.586 kb on + strand at 1003.591 kb on - strand at 1003.613 kb on + strand at 1003.721 kb on + strand, within MMJJ_RS05405 at 1003.726 kb on + strand, within MMJJ_RS05405 at 1003.727 kb on - strand, within MMJJ_RS05405 at 1003.727 kb on - strand, within MMJJ_RS05405 at 1003.728 kb on + strand, within MMJJ_RS05405 at 1003.761 kb on + strand, within MMJJ_RS05405 at 1003.814 kb on - strand, within MMJJ_RS05405 at 1003.838 kb on - strand, within MMJJ_RS05405 at 1003.864 kb on - strand, within MMJJ_RS05405 at 1003.868 kb on - strand, within MMJJ_RS05405 at 1003.868 kb on - strand, within MMJJ_RS05405 at 1003.869 kb on + strand, within MMJJ_RS05405 at 1003.879 kb on - strand, within MMJJ_RS05405 at 1003.916 kb on - strand, within MMJJ_RS05405 at 1003.922 kb on - strand, within MMJJ_RS05405 at 1003.923 kb on + strand, within MMJJ_RS05405 at 1003.930 kb on + strand, within MMJJ_RS05405 at 1003.952 kb on + strand, within MMJJ_RS05405 at 1003.974 kb on - strand, within MMJJ_RS05405 at 1003.975 kb on + strand, within MMJJ_RS05405 at 1004.012 kb on - strand, within MMJJ_RS05405 at 1004.013 kb on + strand, within MMJJ_RS05405 at 1004.051 kb on + strand, within MMJJ_RS05405 at 1004.055 kb on - strand, within MMJJ_RS05405 at 1004.055 kb on - strand, within MMJJ_RS05405 at 1004.056 kb on + strand, within MMJJ_RS05405 at 1004.057 kb on - strand, within MMJJ_RS05405 at 1004.060 kb on - strand, within MMJJ_RS05405 at 1004.061 kb on + strand, within MMJJ_RS05405 at 1004.075 kb on - strand, within MMJJ_RS05405 at 1004.076 kb on + strand, within MMJJ_RS05405 at 1004.076 kb on + strand, within MMJJ_RS05405 at 1004.095 kb on - strand, within MMJJ_RS05405 at 1004.133 kb on + strand, within MMJJ_RS05405 at 1004.133 kb on + strand, within MMJJ_RS05405 at 1004.142 kb on + strand, within MMJJ_RS05405 at 1004.181 kb on - strand, within MMJJ_RS05405 at 1004.240 kb on - strand, within MMJJ_RS05405 at 1004.276 kb on - strand, within MMJJ_RS05405 at 1004.288 kb on + strand, within MMJJ_RS05405 at 1004.312 kb on - strand, within MMJJ_RS05405 at 1004.399 kb on - strand, within MMJJ_RS05405 at 1004.425 kb on - strand, within MMJJ_RS05405 at 1004.426 kb on + strand, within MMJJ_RS05405 at 1004.433 kb on - strand, within MMJJ_RS05405 at 1004.433 kb on - strand, within MMJJ_RS05405 at 1004.433 kb on - strand, within MMJJ_RS05405 at 1004.433 kb on - strand, within MMJJ_RS05405 at 1004.434 kb on + strand, within MMJJ_RS05405 at 1004.434 kb on + strand, within MMJJ_RS05405 at 1004.489 kb on - strand, within MMJJ_RS05405 at 1004.489 kb on - strand, within MMJJ_RS05405 at 1004.489 kb on - strand, within MMJJ_RS05405 at 1004.489 kb on - strand, within MMJJ_RS05405 at 1004.516 kb on + strand, within MMJJ_RS05405
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,002,092 - MMJJ_RS05395 0.34 -2.3 1,003,071 - -1.5 1,003,103 - +1.2 1,003,103 - +1.4 1,003,202 - MMJJ_RS05400 0.26 +0.3 1,003,203 + MMJJ_RS05400 0.27 -2.5 1,003,203 + MMJJ_RS05400 0.27 +0.9 1,003,254 - MMJJ_RS05400 0.38 -0.6 1,003,308 - MMJJ_RS05400 0.50 +0.9 1,003,309 + MMJJ_RS05400 0.50 +0.6 1,003,309 + MMJJ_RS05400 0.50 +0.6 1,003,309 + MMJJ_RS05400 0.50 +1.2 1,003,321 + MMJJ_RS05400 0.53 +0.6 1,003,365 + MMJJ_RS05400 0.63 +1.0 1,003,399 - MMJJ_RS05400 0.70 -0.0 1,003,399 - MMJJ_RS05400 0.70 -0.4 1,003,515 + -0.4 1,003,534 - +0.2 1,003,534 - +0.7 1,003,534 - +1.1 1,003,534 - -0.1 1,003,534 - +1.3 1,003,534 - -0.7 1,003,535 + -0.4 1,003,535 + +0.5 1,003,545 - +0.2 1,003,546 + +1.1 1,003,546 + +0.1 1,003,546 + -1.5 1,003,553 - -1.8 1,003,554 + -0.8 1,003,554 + -0.4 1,003,557 - -0.9 1,003,557 - -1.7 1,003,567 - -0.1 1,003,568 + -0.2 1,003,568 + +0.3 1,003,568 + -0.3 1,003,568 + +0.4 1,003,568 + +1.1 1,003,568 + +1.1 1,003,569 - -0.7 1,003,569 - -0.6 1,003,569 - +0.8 1,003,570 + +1.0 1,003,570 + +1.3 1,003,586 + -0.2 1,003,591 - -0.7 1,003,613 + +0.5 1,003,721 + MMJJ_RS05405 0.11 +1.2 1,003,726 + MMJJ_RS05405 0.12 -1.1 1,003,727 - MMJJ_RS05405 0.12 -0.2 1,003,727 - MMJJ_RS05405 0.12 +1.4 1,003,728 + MMJJ_RS05405 0.12 -0.9 1,003,761 + MMJJ_RS05405 0.15 -0.8 1,003,814 - MMJJ_RS05405 0.20 +0.5 1,003,838 - MMJJ_RS05405 0.22 -0.1 1,003,864 - MMJJ_RS05405 0.25 +1.4 1,003,868 - MMJJ_RS05405 0.25 -0.3 1,003,868 - MMJJ_RS05405 0.25 +0.2 1,003,869 + MMJJ_RS05405 0.25 +0.8 1,003,879 - MMJJ_RS05405 0.26 -0.2 1,003,916 - MMJJ_RS05405 0.29 -0.9 1,003,922 - MMJJ_RS05405 0.30 -0.5 1,003,923 + MMJJ_RS05405 0.30 -0.6 1,003,930 + MMJJ_RS05405 0.31 +0.3 1,003,952 + MMJJ_RS05405 0.33 +1.4 1,003,974 - MMJJ_RS05405 0.35 +0.3 1,003,975 + MMJJ_RS05405 0.35 +1.3 1,004,012 - MMJJ_RS05405 0.38 -0.1 1,004,013 + MMJJ_RS05405 0.38 +0.1 1,004,051 + MMJJ_RS05405 0.42 -0.1 1,004,055 - MMJJ_RS05405 0.42 +0.5 1,004,055 - MMJJ_RS05405 0.42 +0.6 1,004,056 + MMJJ_RS05405 0.43 +0.6 1,004,057 - MMJJ_RS05405 0.43 -0.0 1,004,060 - MMJJ_RS05405 0.43 +0.8 1,004,061 + MMJJ_RS05405 0.43 +0.7 1,004,075 - MMJJ_RS05405 0.44 +0.8 1,004,076 + MMJJ_RS05405 0.44 +0.1 1,004,076 + MMJJ_RS05405 0.44 -0.3 1,004,095 - MMJJ_RS05405 0.46 +0.4 1,004,133 + MMJJ_RS05405 0.50 -1.9 1,004,133 + MMJJ_RS05405 0.50 -0.1 1,004,142 + MMJJ_RS05405 0.51 -0.0 1,004,181 - MMJJ_RS05405 0.54 +0.1 1,004,240 - MMJJ_RS05405 0.60 -0.1 1,004,276 - MMJJ_RS05405 0.63 -3.1 1,004,288 + MMJJ_RS05405 0.64 +1.3 1,004,312 - MMJJ_RS05405 0.66 +0.2 1,004,399 - MMJJ_RS05405 0.75 -2.3 1,004,425 - MMJJ_RS05405 0.77 -0.2 1,004,426 + MMJJ_RS05405 0.77 +0.0 1,004,433 - MMJJ_RS05405 0.78 +1.9 1,004,433 - MMJJ_RS05405 0.78 +1.2 1,004,433 - MMJJ_RS05405 0.78 +1.2 1,004,433 - MMJJ_RS05405 0.78 -0.2 1,004,434 + MMJJ_RS05405 0.78 +3.0 1,004,434 + MMJJ_RS05405 0.78 +0.5 1,004,489 - MMJJ_RS05405 0.83 +0.8 1,004,489 - MMJJ_RS05405 0.83 -0.8 1,004,489 - MMJJ_RS05405 0.83 +1.3 1,004,489 - MMJJ_RS05405 0.83 -0.3 1,004,516 + MMJJ_RS05405 0.86 -1.0
Or see this region's nucleotide sequence