Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS04455

Experiment: H2 N-free with 10mM Ammonium chloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS04450 and MMJJ_RS04455 are separated by 89 nucleotidesMMJJ_RS04455 and MMJJ_RS04460 are separated by 72 nucleotidesMMJJ_RS04460 and MMJJ_RS04465 are separated by 11 nucleotides MMJJ_RS04450: MMJJ_RS04450 - diphthamide biosynthesis enzyme Dph2, at 828,864 to 829,793 _RS04450 MMJJ_RS04455: MMJJ_RS04455 - phosphomethylpyrimidine synthase, at 829,883 to 831,163 _RS04455 MMJJ_RS04460: MMJJ_RS04460 - L-threonylcarbamoyladenylate synthase, at 831,236 to 831,856 _RS04460 MMJJ_RS04465: MMJJ_RS04465 - MTAP family purine nucleoside phosphorylase, at 831,868 to 832,629 _RS04465 Position (kb) 829 830 831 832Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 828.922 kb on - strandat 829.004 kb on - strand, within MMJJ_RS04450at 829.009 kb on - strand, within MMJJ_RS04450at 829.010 kb on + strand, within MMJJ_RS04450at 829.010 kb on + strand, within MMJJ_RS04450at 829.010 kb on + strand, within MMJJ_RS04450at 829.030 kb on + strand, within MMJJ_RS04450at 829.044 kb on - strand, within MMJJ_RS04450at 829.044 kb on - strand, within MMJJ_RS04450at 829.045 kb on + strand, within MMJJ_RS04450at 829.068 kb on + strand, within MMJJ_RS04450at 829.068 kb on + strand, within MMJJ_RS04450at 829.068 kb on + strand, within MMJJ_RS04450at 829.070 kb on + strand, within MMJJ_RS04450at 829.079 kb on - strand, within MMJJ_RS04450at 829.090 kb on + strand, within MMJJ_RS04450at 829.180 kb on + strand, within MMJJ_RS04450at 829.182 kb on - strand, within MMJJ_RS04450at 829.183 kb on + strand, within MMJJ_RS04450at 829.259 kb on - strand, within MMJJ_RS04450at 829.260 kb on + strand, within MMJJ_RS04450at 829.290 kb on - strand, within MMJJ_RS04450at 829.296 kb on + strand, within MMJJ_RS04450at 829.297 kb on - strand, within MMJJ_RS04450at 829.297 kb on - strand, within MMJJ_RS04450at 829.298 kb on + strand, within MMJJ_RS04450at 829.307 kb on - strand, within MMJJ_RS04450at 829.307 kb on - strandat 829.308 kb on + strand, within MMJJ_RS04450at 829.308 kb on + strand, within MMJJ_RS04450at 829.384 kb on + strand, within MMJJ_RS04450at 829.572 kb on + strand, within MMJJ_RS04450at 829.765 kb on + strandat 829.772 kb on - strandat 829.773 kb on + strandat 829.794 kb on + strandat 829.794 kb on + strandat 829.796 kb on - strandat 829.797 kb on + strandat 829.813 kb on + strandat 829.816 kb on - strandat 829.828 kb on + strandat 829.828 kb on + strandat 829.828 kb on + strandat 829.828 kb on + strandat 829.828 kb on + strandat 829.832 kb on + strandat 829.832 kb on + strandat 829.834 kb on - strandat 829.834 kb on - strandat 829.835 kb on + strandat 829.838 kb on + strandat 829.849 kb on - strandat 829.869 kb on + strandat 829.871 kb on + strandat 830.174 kb on + strandat 830.249 kb on - strand, within MMJJ_RS04455at 830.858 kb on - strand, within MMJJ_RS04455at 830.904 kb on - strand, within MMJJ_RS04455at 831.135 kb on - strandat 831.136 kb on + strandat 831.164 kb on - strandat 831.165 kb on + strandat 831.165 kb on + strandat 831.166 kb on - strandat 831.166 kb on - strandat 831.166 kb on - strandat 831.166 kb on - strandat 831.188 kb on + strandat 831.190 kb on - strandat 831.193 kb on + strandat 831.193 kb on + strandat 831.246 kb on + strandat 831.254 kb on - strandat 831.477 kb on + strand, within MMJJ_RS04460at 831.731 kb on + strand, within MMJJ_RS04460at 831.830 kb on + strandat 831.856 kb on - strandat 831.856 kb on - strandat 831.869 kb on - strandat 831.870 kb on + strandat 831.870 kb on + strandat 831.927 kb on + strandat 831.931 kb on + strandat 831.931 kb on + strandat 831.933 kb on - strandat 831.933 kb on - strandat 831.934 kb on + strandat 832.014 kb on - strand, within MMJJ_RS04465at 832.081 kb on - strand, within MMJJ_RS04465at 832.082 kb on + strand, within MMJJ_RS04465at 832.087 kb on - strand, within MMJJ_RS04465at 832.088 kb on + strand, within MMJJ_RS04465

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride
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828,922 - -0.9
829,004 - MMJJ_RS04450 0.15 +0.3
829,009 - MMJJ_RS04450 0.16 -1.5
829,010 + MMJJ_RS04450 0.16 +1.2
829,010 + MMJJ_RS04450 0.16 -1.2
829,010 + MMJJ_RS04450 0.16 -0.6
829,030 + MMJJ_RS04450 0.18 -0.7
829,044 - MMJJ_RS04450 0.19 -0.7
829,044 - MMJJ_RS04450 0.19 -0.4
829,045 + MMJJ_RS04450 0.19 -0.5
829,068 + MMJJ_RS04450 0.22 -0.0
829,068 + MMJJ_RS04450 0.22 +0.2
829,068 + MMJJ_RS04450 0.22 -0.4
829,070 + MMJJ_RS04450 0.22 -1.9
829,079 - MMJJ_RS04450 0.23 -0.5
829,090 + MMJJ_RS04450 0.24 +0.7
829,180 + MMJJ_RS04450 0.34 -0.3
829,182 - MMJJ_RS04450 0.34 -2.0
829,183 + MMJJ_RS04450 0.34 +0.6
829,259 - MMJJ_RS04450 0.42 +0.3
829,260 + MMJJ_RS04450 0.43 -1.0
829,290 - MMJJ_RS04450 0.46 -0.4
829,296 + MMJJ_RS04450 0.46 -0.8
829,297 - MMJJ_RS04450 0.47 +0.2
829,297 - MMJJ_RS04450 0.47 -1.6
829,298 + MMJJ_RS04450 0.47 -0.6
829,307 - MMJJ_RS04450 0.48 -2.5
829,307 - +1.2
829,308 + MMJJ_RS04450 0.48 -1.8
829,308 + MMJJ_RS04450 0.48 +0.2
829,384 + MMJJ_RS04450 0.56 -0.4
829,572 + MMJJ_RS04450 0.76 -1.1
829,765 + -0.6
829,772 - -0.8
829,773 + -3.9
829,794 + +0.3
829,794 + +0.6
829,796 - -0.3
829,797 + -0.6
829,813 + +0.3
829,816 - +0.0
829,828 + -0.0
829,828 + -0.1
829,828 + -0.6
829,828 + -0.6
829,828 + -1.1
829,832 + -2.0
829,832 + -1.3
829,834 - +0.2
829,834 - -2.9
829,835 + +0.3
829,838 + -0.4
829,849 - -2.0
829,869 + -1.8
829,871 + -2.4
830,174 + -0.8
830,249 - MMJJ_RS04455 0.29 -0.4
830,858 - MMJJ_RS04455 0.76 -1.8
830,904 - MMJJ_RS04455 0.80 -0.4
831,135 - -1.3
831,136 + -1.9
831,164 - -0.5
831,165 + +0.7
831,165 + -0.5
831,166 - -1.1
831,166 - -0.2
831,166 - +0.8
831,166 - +0.4
831,188 + +1.2
831,190 - +1.2
831,193 + +0.4
831,193 + -0.0
831,246 + -0.7
831,254 - -1.9
831,477 + MMJJ_RS04460 0.39 -3.0
831,731 + MMJJ_RS04460 0.80 -3.4
831,830 + -0.8
831,856 - +0.2
831,856 - -2.1
831,869 - +0.4
831,870 + +0.9
831,870 + -1.5
831,927 + -1.0
831,931 + -1.9
831,931 + +0.1
831,933 - +0.6
831,933 - -0.0
831,934 + -1.2
832,014 - MMJJ_RS04465 0.19 +0.3
832,081 - MMJJ_RS04465 0.28 -0.2
832,082 + MMJJ_RS04465 0.28 -1.3
832,087 - MMJJ_RS04465 0.29 -1.8
832,088 + MMJJ_RS04465 0.29 -0.7

Or see this region's nucleotide sequence