Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03950 and MMJJ_RS03955 are separated by 232 nucleotides MMJJ_RS03955 and MMJJ_RS03960 are separated by 668 nucleotides
MMJJ_RS03950: MMJJ_RS03950 - tyrosine--tRNA ligase, at 737,024 to 737,953
_RS03950
MMJJ_RS03955: MMJJ_RS03955 - hypothetical protein, at 738,186 to 738,383
_RS03955
MMJJ_RS03960: MMJJ_RS03960 - DUF2067 domain-containing protein, at 739,052 to 739,669
_RS03960
Position (kb) 738
739 Strain fitness (log2 ratio) -2
-1
0
1
2 at 737.901 kb on + strand at 737.941 kb on - strand at 738.035 kb on - strand at 738.047 kb on - strand at 738.048 kb on + strand at 738.050 kb on - strand at 738.050 kb on - strand at 738.071 kb on - strand at 738.072 kb on + strand at 738.073 kb on - strand at 738.092 kb on - strand at 738.173 kb on - strand at 738.174 kb on + strand at 738.178 kb on - strand at 738.179 kb on + strand at 738.183 kb on + strand at 738.231 kb on + strand, within MMJJ_RS03955 at 738.233 kb on - strand, within MMJJ_RS03955 at 738.233 kb on - strand, within MMJJ_RS03955 at 738.233 kb on - strand, within MMJJ_RS03955 at 738.233 kb on - strand, within MMJJ_RS03955 at 738.233 kb on - strand, within MMJJ_RS03955 at 738.234 kb on + strand, within MMJJ_RS03955 at 738.234 kb on + strand, within MMJJ_RS03955 at 738.234 kb on + strand, within MMJJ_RS03955 at 738.234 kb on + strand, within MMJJ_RS03955 at 738.234 kb on + strand, within MMJJ_RS03955 at 738.234 kb on + strand, within MMJJ_RS03955 at 738.235 kb on - strand, within MMJJ_RS03955 at 738.235 kb on - strand, within MMJJ_RS03955 at 738.235 kb on - strand, within MMJJ_RS03955 at 738.237 kb on - strand, within MMJJ_RS03955 at 738.237 kb on - strand, within MMJJ_RS03955 at 738.237 kb on - strand, within MMJJ_RS03955 at 738.237 kb on - strand, within MMJJ_RS03955 at 738.238 kb on + strand, within MMJJ_RS03955 at 738.243 kb on + strand, within MMJJ_RS03955 at 738.288 kb on - strand, within MMJJ_RS03955 at 738.289 kb on + strand, within MMJJ_RS03955 at 738.293 kb on - strand, within MMJJ_RS03955 at 738.293 kb on - strand, within MMJJ_RS03955 at 738.311 kb on - strand, within MMJJ_RS03955 at 738.329 kb on - strand, within MMJJ_RS03955 at 738.378 kb on - strand at 738.378 kb on - strand at 738.378 kb on - strand at 738.378 kb on - strand at 738.400 kb on + strand at 738.401 kb on - strand at 738.402 kb on + strand at 738.402 kb on + strand at 738.402 kb on + strand at 738.402 kb on + strand at 738.405 kb on - strand at 738.405 kb on - strand at 738.405 kb on - strand at 738.406 kb on + strand at 738.406 kb on + strand at 738.407 kb on - strand at 738.407 kb on - strand at 738.407 kb on - strand at 738.407 kb on - strand at 738.408 kb on + strand at 738.408 kb on + strand at 738.408 kb on + strand at 738.408 kb on + strand at 738.408 kb on + strand at 738.408 kb on + strand at 738.408 kb on + strand at 738.408 kb on + strand at 738.427 kb on - strand at 738.428 kb on + strand at 738.428 kb on + strand at 738.561 kb on - strand at 738.600 kb on - strand at 738.606 kb on + strand at 738.624 kb on - strand at 738.714 kb on - strand at 738.740 kb on + strand at 738.740 kb on + strand at 738.753 kb on - strand at 738.753 kb on - strand at 738.753 kb on - strand at 738.779 kb on - strand at 738.785 kb on - strand at 738.785 kb on - strand at 738.785 kb on - strand at 738.786 kb on + strand at 738.786 kb on + strand at 738.786 kb on + strand at 738.796 kb on - strand at 738.796 kb on - strand at 738.800 kb on - strand at 738.818 kb on - strand at 738.869 kb on - strand at 738.869 kb on - strand at 738.870 kb on + strand at 738.870 kb on + strand at 738.870 kb on + strand at 738.871 kb on - strand at 738.871 kb on - strand at 738.874 kb on + strand at 738.874 kb on + strand at 738.874 kb on + strand at 738.874 kb on + strand at 738.874 kb on + strand at 738.918 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.950 kb on - strand at 738.951 kb on + strand at 738.951 kb on + strand at 738.951 kb on + strand at 738.951 kb on + strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.955 kb on - strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.956 kb on + strand at 738.957 kb on - strand at 738.958 kb on + strand at 738.958 kb on + strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.961 kb on - strand at 738.962 kb on + strand at 738.962 kb on + strand at 738.962 kb on + strand at 738.962 kb on + strand at 738.962 kb on + strand at 738.962 kb on + strand at 738.965 kb on - strand at 738.965 kb on - strand at 738.965 kb on - strand at 738.965 kb on - strand at 738.966 kb on + strand at 738.966 kb on + strand at 738.966 kb on + strand at 738.966 kb on + strand at 738.971 kb on - strand at 738.971 kb on - strand at 738.971 kb on - strand at 738.972 kb on + strand at 738.972 kb on + strand at 738.972 kb on + strand at 738.972 kb on + strand at 738.972 kb on + strand at 738.972 kb on + strand at 738.976 kb on + strand at 738.976 kb on + strand at 738.976 kb on + strand at 738.976 kb on + strand at 738.976 kb on + strand at 738.987 kb on - strand at 738.988 kb on + strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.004 kb on - strand at 739.005 kb on + strand at 739.005 kb on + strand at 739.007 kb on + strand at 739.007 kb on + strand at 739.007 kb on + strand at 739.010 kb on - strand at 739.010 kb on - strand at 739.011 kb on + strand at 739.011 kb on + strand at 739.062 kb on - strand at 739.063 kb on + strand at 739.063 kb on + strand at 739.066 kb on + strand at 739.066 kb on + strand at 739.066 kb on + strand at 739.072 kb on + strand at 739.113 kb on - strand at 739.117 kb on - strand, within MMJJ_RS03960 at 739.117 kb on - strand, within MMJJ_RS03960 at 739.118 kb on + strand, within MMJJ_RS03960 at 739.118 kb on + strand, within MMJJ_RS03960 at 739.175 kb on - strand, within MMJJ_RS03960 at 739.176 kb on + strand, within MMJJ_RS03960 at 739.178 kb on + strand, within MMJJ_RS03960 at 739.178 kb on + strand, within MMJJ_RS03960 at 739.198 kb on + strand, within MMJJ_RS03960 at 739.224 kb on - strand, within MMJJ_RS03960 at 739.246 kb on - strand, within MMJJ_RS03960 at 739.246 kb on - strand, within MMJJ_RS03960 at 739.246 kb on - strand, within MMJJ_RS03960 at 739.247 kb on + strand, within MMJJ_RS03960 at 739.247 kb on + strand, within MMJJ_RS03960 at 739.250 kb on + strand, within MMJJ_RS03960 at 739.253 kb on + strand, within MMJJ_RS03960 at 739.254 kb on - strand, within MMJJ_RS03960 at 739.255 kb on + strand, within MMJJ_RS03960 at 739.255 kb on + strand, within MMJJ_RS03960 at 739.258 kb on - strand, within MMJJ_RS03960 at 739.258 kb on - strand, within MMJJ_RS03960 at 739.271 kb on + strand, within MMJJ_RS03960 at 739.271 kb on + strand, within MMJJ_RS03960 at 739.320 kb on - strand, within MMJJ_RS03960 at 739.326 kb on + strand, within MMJJ_RS03960 at 739.368 kb on - strand, within MMJJ_RS03960 at 739.382 kb on + strand, within MMJJ_RS03960
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 737,901 + -1.9 737,941 - -2.0 738,035 - -1.9 738,047 - -1.4 738,048 + -1.4 738,050 - -1.2 738,050 - -1.3 738,071 - -1.2 738,072 + -1.4 738,073 - +0.5 738,092 - +1.0 738,173 - -1.1 738,174 + -0.2 738,178 - +0.2 738,179 + +0.8 738,183 + +1.3 738,231 + MMJJ_RS03955 0.23 -1.4 738,233 - MMJJ_RS03955 0.24 +1.1 738,233 - MMJJ_RS03955 0.24 -0.1 738,233 - MMJJ_RS03955 0.24 +1.3 738,233 - MMJJ_RS03955 0.24 +1.0 738,233 - MMJJ_RS03955 0.24 +0.1 738,234 + MMJJ_RS03955 0.24 -1.3 738,234 + MMJJ_RS03955 0.24 +0.3 738,234 + MMJJ_RS03955 0.24 +1.7 738,234 + MMJJ_RS03955 0.24 -0.2 738,234 + MMJJ_RS03955 0.24 +1.0 738,234 + MMJJ_RS03955 0.24 +0.5 738,235 - MMJJ_RS03955 0.25 -1.0 738,235 - MMJJ_RS03955 0.25 +1.4 738,235 - MMJJ_RS03955 0.25 +0.4 738,237 - MMJJ_RS03955 0.26 +2.2 738,237 - MMJJ_RS03955 0.26 +0.7 738,237 - MMJJ_RS03955 0.26 -0.6 738,237 - MMJJ_RS03955 0.26 -0.9 738,238 + MMJJ_RS03955 0.26 +0.2 738,243 + MMJJ_RS03955 0.29 -0.8 738,288 - MMJJ_RS03955 0.52 +0.6 738,289 + MMJJ_RS03955 0.52 +1.0 738,293 - MMJJ_RS03955 0.54 -1.0 738,293 - MMJJ_RS03955 0.54 +0.3 738,311 - MMJJ_RS03955 0.63 -2.0 738,329 - MMJJ_RS03955 0.72 +0.6 738,378 - -0.7 738,378 - +0.4 738,378 - +0.2 738,378 - +0.2 738,400 + -0.4 738,401 - +1.0 738,402 + -1.8 738,402 + -0.3 738,402 + -0.7 738,402 + +0.1 738,405 - -2.0 738,405 - -0.1 738,405 - +0.4 738,406 + +0.7 738,406 + +0.4 738,407 - +0.9 738,407 - +0.2 738,407 - +0.2 738,407 - -1.0 738,408 + -0.9 738,408 + -0.6 738,408 + +0.9 738,408 + +1.9 738,408 + +0.6 738,408 + -1.9 738,408 + +0.0 738,408 + +0.0 738,427 - -1.3 738,428 + -0.9 738,428 + +0.6 738,561 - -0.1 738,600 - -0.1 738,606 + -0.4 738,624 - -0.9 738,714 - -0.9 738,740 + -0.2 738,740 + -0.5 738,753 - +1.8 738,753 - +0.1 738,753 - +0.9 738,779 - +0.6 738,785 - +1.1 738,785 - -0.1 738,785 - -1.0 738,786 + -2.0 738,786 + -0.7 738,786 + -0.1 738,796 - -2.6 738,796 - +0.6 738,800 - +0.1 738,818 - -0.6 738,869 - +0.5 738,869 - -0.8 738,870 + +0.8 738,870 + +0.9 738,870 + -0.6 738,871 - -0.1 738,871 - -0.3 738,874 + +0.6 738,874 + -0.0 738,874 + +0.0 738,874 + -0.1 738,874 + +1.6 738,918 - -0.9 738,950 - +1.2 738,950 - -0.5 738,950 - -0.7 738,950 - +1.1 738,950 - -0.7 738,950 - -0.3 738,950 - +0.7 738,950 - -0.3 738,951 + +0.1 738,951 + -0.4 738,951 + +0.5 738,951 + -1.7 738,955 - -1.0 738,955 - -1.4 738,955 - +0.9 738,955 - -0.1 738,955 - +0.8 738,955 - -0.9 738,955 - +0.0 738,956 + -0.9 738,956 + +1.4 738,956 + -0.5 738,956 + -0.7 738,956 + -0.9 738,956 + -1.0 738,956 + -0.6 738,956 + +0.5 738,956 + +1.1 738,956 + +0.6 738,957 - +1.1 738,958 + +0.0 738,958 + +0.4 738,961 - -0.2 738,961 - -2.7 738,961 - -0.1 738,961 - +1.6 738,961 - -0.9 738,961 - +0.1 738,961 - +0.7 738,961 - +0.7 738,962 + -0.7 738,962 + -0.7 738,962 + +0.5 738,962 + -0.5 738,962 + +0.4 738,962 + -0.4 738,965 - -2.3 738,965 - +0.2 738,965 - +1.7 738,965 - +0.9 738,966 + +1.2 738,966 + -0.9 738,966 + +0.7 738,966 + +1.0 738,971 - -0.7 738,971 - -1.7 738,971 - -1.6 738,972 + +0.5 738,972 + -1.4 738,972 + +0.7 738,972 + -2.3 738,972 + -2.0 738,972 + -0.3 738,976 + +0.4 738,976 + +0.7 738,976 + -1.7 738,976 + +1.5 738,976 + -1.1 738,987 - -0.9 738,988 + +0.9 739,004 - -0.0 739,004 - -1.5 739,004 - +0.7 739,004 - +0.4 739,004 - +0.0 739,004 - +0.3 739,004 - +0.6 739,004 - -1.6 739,004 - -0.6 739,005 + +0.7 739,005 + +0.2 739,007 + -0.8 739,007 + -1.1 739,007 + -0.4 739,010 - +1.1 739,010 - +0.6 739,011 + -0.4 739,011 + -0.9 739,062 - +0.4 739,063 + -0.3 739,063 + -0.1 739,066 + -0.3 739,066 + -1.9 739,066 + -0.9 739,072 + +0.5 739,113 - +0.2 739,117 - MMJJ_RS03960 0.11 +0.0 739,117 - MMJJ_RS03960 0.11 -1.9 739,118 + MMJJ_RS03960 0.11 -0.7 739,118 + MMJJ_RS03960 0.11 -0.7 739,175 - MMJJ_RS03960 0.20 +0.1 739,176 + MMJJ_RS03960 0.20 +0.8 739,178 + MMJJ_RS03960 0.20 -0.1 739,178 + MMJJ_RS03960 0.20 -0.4 739,198 + MMJJ_RS03960 0.24 +0.7 739,224 - MMJJ_RS03960 0.28 -0.8 739,246 - MMJJ_RS03960 0.31 -0.9 739,246 - MMJJ_RS03960 0.31 +1.2 739,246 - MMJJ_RS03960 0.31 -1.3 739,247 + MMJJ_RS03960 0.32 +1.2 739,247 + MMJJ_RS03960 0.32 +0.3 739,250 + MMJJ_RS03960 0.32 +0.3 739,253 + MMJJ_RS03960 0.33 +0.7 739,254 - MMJJ_RS03960 0.33 +0.4 739,255 + MMJJ_RS03960 0.33 +0.4 739,255 + MMJJ_RS03960 0.33 +0.8 739,258 - MMJJ_RS03960 0.33 -2.0 739,258 - MMJJ_RS03960 0.33 -0.4 739,271 + MMJJ_RS03960 0.35 +2.0 739,271 + MMJJ_RS03960 0.35 +0.0 739,320 - MMJJ_RS03960 0.43 +2.4 739,326 + MMJJ_RS03960 0.44 +1.1 739,368 - MMJJ_RS03960 0.51 -1.0 739,382 + MMJJ_RS03960 0.53 -0.1
Or see this region's nucleotide sequence