Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03595 and MMJJ_RS03600 are separated by 70 nucleotides MMJJ_RS03600 and MMJJ_RS03605 are separated by 161 nucleotides MMJJ_RS03605 and MMJJ_RS03610 are separated by 436 nucleotides MMJJ_RS03610 and MMJJ_RS03615 are separated by 1 nucleotides
MMJJ_RS03595: MMJJ_RS03595 - hypothetical protein, at 670,823 to 671,236
_RS03595
MMJJ_RS03600: MMJJ_RS03600 - TrmJ/YjtD family RNA methyltransferase, at 671,307 to 672,011
_RS03600
MMJJ_RS03605: MMJJ_RS03605 - TIGR00297 family protein, at 672,173 to 672,886
_RS03605
MMJJ_RS03610: MMJJ_RS03610 - HIT family protein, at 673,323 to 673,715
_RS03610
MMJJ_RS03615: MMJJ_RS03615 - cobalt-precorrin-8 methylmutase, at 673,717 to 674,349
_RS03615
Position (kb) 672
673 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 671.229 kb on + strand at 671.229 kb on + strand at 671.229 kb on + strand at 671.229 kb on + strand at 671.229 kb on + strand at 671.230 kb on - strand at 671.230 kb on - strand at 671.230 kb on - strand at 671.257 kb on - strand at 671.257 kb on - strand at 671.258 kb on + strand at 671.261 kb on + strand at 671.266 kb on - strand at 671.270 kb on + strand at 671.276 kb on + strand at 671.276 kb on + strand at 671.309 kb on + strand at 671.321 kb on - strand at 671.322 kb on + strand at 671.328 kb on + strand at 671.337 kb on - strand at 671.338 kb on + strand at 671.341 kb on + strand at 671.391 kb on + strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.406 kb on - strand, within MMJJ_RS03600 at 671.407 kb on + strand, within MMJJ_RS03600 at 671.407 kb on + strand, within MMJJ_RS03600 at 671.417 kb on - strand, within MMJJ_RS03600 at 671.556 kb on - strand, within MMJJ_RS03600 at 671.640 kb on - strand, within MMJJ_RS03600 at 671.640 kb on - strand, within MMJJ_RS03600 at 671.641 kb on + strand, within MMJJ_RS03600 at 671.682 kb on - strand, within MMJJ_RS03600 at 671.735 kb on - strand, within MMJJ_RS03600 at 671.751 kb on - strand, within MMJJ_RS03600 at 671.792 kb on - strand, within MMJJ_RS03600 at 671.793 kb on + strand, within MMJJ_RS03600 at 671.852 kb on - strand, within MMJJ_RS03600 at 671.876 kb on - strand, within MMJJ_RS03600 at 671.876 kb on - strand, within MMJJ_RS03600 at 671.877 kb on + strand, within MMJJ_RS03600 at 671.877 kb on + strand, within MMJJ_RS03600 at 671.878 kb on - strand, within MMJJ_RS03600 at 671.878 kb on - strand, within MMJJ_RS03600 at 671.879 kb on + strand, within MMJJ_RS03600 at 671.879 kb on + strand, within MMJJ_RS03600 at 671.879 kb on + strand, within MMJJ_RS03600 at 671.879 kb on + strand, within MMJJ_RS03600 at 671.913 kb on - strand, within MMJJ_RS03600 at 671.978 kb on + strand at 671.996 kb on - strand at 671.996 kb on - strand at 671.997 kb on + strand at 672.057 kb on + strand at 672.062 kb on - strand at 672.062 kb on - strand at 672.062 kb on - strand at 672.063 kb on + strand at 672.067 kb on - strand at 672.067 kb on - strand at 672.067 kb on - strand at 672.068 kb on + strand at 672.079 kb on + strand at 672.079 kb on + strand at 672.082 kb on - strand at 672.082 kb on - strand at 672.083 kb on + strand at 672.085 kb on - strand at 672.089 kb on - strand at 672.090 kb on + strand at 672.121 kb on + strand at 672.169 kb on - strand at 672.174 kb on - strand at 672.174 kb on - strand at 672.180 kb on + strand at 672.180 kb on + strand at 672.189 kb on - strand at 672.190 kb on + strand at 672.218 kb on - strand at 672.219 kb on + strand at 672.219 kb on + strand at 672.254 kb on - strand, within MMJJ_RS03605 at 672.342 kb on + strand, within MMJJ_RS03605 at 672.369 kb on + strand, within MMJJ_RS03605 at 672.544 kb on + strand, within MMJJ_RS03605 at 672.548 kb on - strand, within MMJJ_RS03605 at 672.548 kb on - strand, within MMJJ_RS03605 at 672.692 kb on + strand, within MMJJ_RS03605 at 672.743 kb on - strand, within MMJJ_RS03605 at 672.766 kb on - strand, within MMJJ_RS03605 at 672.766 kb on - strand, within MMJJ_RS03605 at 672.827 kb on - strand at 672.866 kb on - strand at 672.899 kb on + strand at 672.921 kb on + strand at 672.921 kb on + strand at 672.923 kb on - strand at 672.930 kb on - strand at 672.935 kb on - strand at 672.939 kb on - strand at 672.939 kb on - strand at 672.945 kb on + strand at 672.959 kb on - strand at 672.962 kb on - strand at 672.962 kb on - strand at 672.962 kb on - strand at 672.963 kb on + strand at 672.964 kb on - strand at 672.964 kb on - strand at 672.965 kb on + strand at 672.965 kb on + strand at 672.967 kb on - strand at 672.967 kb on - strand at 672.968 kb on + strand at 672.968 kb on + strand at 672.968 kb on + strand at 672.968 kb on + strand at 672.969 kb on - strand at 672.970 kb on + strand at 672.988 kb on - strand at 673.024 kb on - strand at 673.028 kb on - strand at 673.040 kb on + strand at 673.045 kb on - strand at 673.045 kb on - strand at 673.090 kb on + strand at 673.101 kb on - strand at 673.113 kb on - strand at 673.145 kb on + strand at 673.145 kb on + strand at 673.162 kb on - strand at 673.197 kb on - strand at 673.229 kb on - strand at 673.230 kb on + strand at 673.249 kb on + strand at 673.249 kb on + strand at 673.254 kb on - strand at 673.255 kb on + strand at 673.272 kb on - strand at 673.272 kb on - strand at 673.279 kb on - strand at 673.308 kb on - strand at 673.308 kb on - strand at 673.309 kb on + strand at 673.309 kb on + strand at 673.309 kb on + strand at 673.318 kb on - strand at 673.328 kb on - strand at 673.329 kb on + strand at 673.356 kb on + strand at 673.369 kb on - strand, within MMJJ_RS03610 at 673.370 kb on + strand, within MMJJ_RS03610 at 673.375 kb on + strand, within MMJJ_RS03610 at 673.400 kb on - strand, within MMJJ_RS03610 at 673.401 kb on + strand, within MMJJ_RS03610 at 673.401 kb on + strand, within MMJJ_RS03610 at 673.401 kb on + strand, within MMJJ_RS03610 at 673.401 kb on + strand, within MMJJ_RS03610 at 673.438 kb on - strand, within MMJJ_RS03610 at 673.439 kb on + strand, within MMJJ_RS03610 at 673.564 kb on - strand, within MMJJ_RS03610 at 673.568 kb on - strand, within MMJJ_RS03610 at 673.569 kb on + strand, within MMJJ_RS03610 at 673.714 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 671,229 + +0.4 671,229 + -0.3 671,229 + -2.3 671,229 + +0.5 671,229 + -0.3 671,230 - -2.5 671,230 - +0.5 671,230 - -2.2 671,257 - +0.0 671,257 - -0.9 671,258 + -1.2 671,261 + -1.1 671,266 - +1.0 671,270 + +0.6 671,276 + +0.5 671,276 + -0.5 671,309 + -0.5 671,321 - +1.0 671,322 + -0.5 671,328 + -1.2 671,337 - +0.9 671,338 + -1.1 671,341 + -0.4 671,391 + MMJJ_RS03600 0.12 +0.8 671,406 - MMJJ_RS03600 0.14 -1.4 671,406 - MMJJ_RS03600 0.14 -0.2 671,406 - MMJJ_RS03600 0.14 +0.4 671,406 - MMJJ_RS03600 0.14 -1.9 671,406 - MMJJ_RS03600 0.14 +0.7 671,406 - MMJJ_RS03600 0.14 +1.5 671,406 - MMJJ_RS03600 0.14 +0.7 671,407 + MMJJ_RS03600 0.14 -0.3 671,407 + MMJJ_RS03600 0.14 +1.0 671,417 - MMJJ_RS03600 0.16 -0.3 671,556 - MMJJ_RS03600 0.35 +1.0 671,640 - MMJJ_RS03600 0.47 -0.7 671,640 - MMJJ_RS03600 0.47 +1.4 671,641 + MMJJ_RS03600 0.47 -0.8 671,682 - MMJJ_RS03600 0.53 -0.5 671,735 - MMJJ_RS03600 0.61 -1.0 671,751 - MMJJ_RS03600 0.63 -0.3 671,792 - MMJJ_RS03600 0.69 +0.4 671,793 + MMJJ_RS03600 0.69 -1.8 671,852 - MMJJ_RS03600 0.77 -1.3 671,876 - MMJJ_RS03600 0.81 -0.1 671,876 - MMJJ_RS03600 0.81 +0.8 671,877 + MMJJ_RS03600 0.81 -0.9 671,877 + MMJJ_RS03600 0.81 -0.9 671,878 - MMJJ_RS03600 0.81 +0.4 671,878 - MMJJ_RS03600 0.81 +0.3 671,879 + MMJJ_RS03600 0.81 -0.2 671,879 + MMJJ_RS03600 0.81 +0.5 671,879 + MMJJ_RS03600 0.81 -0.6 671,879 + MMJJ_RS03600 0.81 -1.4 671,913 - MMJJ_RS03600 0.86 -0.4 671,978 + -0.2 671,996 - +0.7 671,996 - +1.2 671,997 + +0.3 672,057 + +0.5 672,062 - -0.1 672,062 - -0.7 672,062 - +0.8 672,063 + +1.1 672,067 - -0.2 672,067 - +0.8 672,067 - -1.1 672,068 + -0.6 672,079 + -0.9 672,079 + +0.2 672,082 - -0.4 672,082 - -0.6 672,083 + -1.0 672,085 - -1.7 672,089 - -4.0 672,090 + +0.2 672,121 + +0.2 672,169 - +0.8 672,174 - +0.1 672,174 - +0.2 672,180 + -1.2 672,180 + +1.1 672,189 - +0.5 672,190 + -1.0 672,218 - +0.1 672,219 + +0.5 672,219 + +0.9 672,254 - MMJJ_RS03605 0.11 +1.3 672,342 + MMJJ_RS03605 0.24 +0.6 672,369 + MMJJ_RS03605 0.27 -1.4 672,544 + MMJJ_RS03605 0.52 -0.1 672,548 - MMJJ_RS03605 0.53 -0.2 672,548 - MMJJ_RS03605 0.53 -0.8 672,692 + MMJJ_RS03605 0.73 +0.3 672,743 - MMJJ_RS03605 0.80 -1.6 672,766 - MMJJ_RS03605 0.83 -0.3 672,766 - MMJJ_RS03605 0.83 +0.1 672,827 - -1.3 672,866 - +0.0 672,899 + -0.5 672,921 + -0.3 672,921 + +0.7 672,923 - -1.1 672,930 - -1.4 672,935 - +0.1 672,939 - +0.9 672,939 - -0.6 672,945 + -0.8 672,959 - +0.7 672,962 - +1.9 672,962 - -0.5 672,962 - -0.1 672,963 + -0.7 672,964 - +0.8 672,964 - +0.7 672,965 + -0.5 672,965 + -1.2 672,967 - -0.7 672,967 - -1.9 672,968 + -0.8 672,968 + -0.2 672,968 + +0.2 672,968 + +0.5 672,969 - +0.0 672,970 + -1.1 672,988 - -0.1 673,024 - -0.6 673,028 - +1.0 673,040 + -0.9 673,045 - -1.4 673,045 - +0.5 673,090 + -0.4 673,101 - -0.6 673,113 - +0.6 673,145 + -0.6 673,145 + -0.3 673,162 - -0.7 673,197 - -0.4 673,229 - +0.6 673,230 + -1.4 673,249 + -0.2 673,249 + +0.6 673,254 - +0.2 673,255 + +0.9 673,272 - +1.4 673,272 - +0.1 673,279 - -1.2 673,308 - -0.7 673,308 - +1.0 673,309 + -0.8 673,309 + -0.2 673,309 + -0.8 673,318 - -0.1 673,328 - -0.6 673,329 + -0.0 673,356 + +0.5 673,369 - MMJJ_RS03610 0.12 -1.9 673,370 + MMJJ_RS03610 0.12 +0.4 673,375 + MMJJ_RS03610 0.13 +1.1 673,400 - MMJJ_RS03610 0.20 +0.9 673,401 + MMJJ_RS03610 0.20 +0.2 673,401 + MMJJ_RS03610 0.20 -0.0 673,401 + MMJJ_RS03610 0.20 -1.1 673,401 + MMJJ_RS03610 0.20 -1.9 673,438 - MMJJ_RS03610 0.29 -1.4 673,439 + MMJJ_RS03610 0.30 +0.5 673,564 - MMJJ_RS03610 0.61 +0.9 673,568 - MMJJ_RS03610 0.62 +0.3 673,569 + MMJJ_RS03610 0.63 -0.1 673,714 + +0.5
Or see this region's nucleotide sequence