Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02235 and MMJJ_RS02240 are separated by 24 nucleotides MMJJ_RS02240 and MMJJ_RS02245 are separated by 150 nucleotides MMJJ_RS02245 and MMJJ_RS02250 are separated by 0 nucleotides
MMJJ_RS02235: MMJJ_RS02235 - DUF123 domain-containing protein, at 423,548 to 424,618
_RS02235
MMJJ_RS02240: MMJJ_RS02240 - CGGC domain-containing protein, at 424,643 to 425,056
_RS02240
MMJJ_RS02245: MMJJ_RS02245 - DUF432 domain-containing protein, at 425,207 to 425,845
_RS02245
MMJJ_RS02250: MMJJ_RS02250 - mechanosensitive ion channel family protein, at 425,846 to 426,928
_RS02250
Position (kb) 424
425
426 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 423.669 kb on - strand, within MMJJ_RS02235 at 423.872 kb on - strand, within MMJJ_RS02235 at 423.874 kb on - strand, within MMJJ_RS02235 at 423.875 kb on + strand, within MMJJ_RS02235 at 423.946 kb on + strand, within MMJJ_RS02235 at 423.946 kb on + strand, within MMJJ_RS02235 at 423.949 kb on + strand, within MMJJ_RS02235 at 423.971 kb on - strand, within MMJJ_RS02235 at 424.006 kb on - strand, within MMJJ_RS02235 at 424.006 kb on - strand, within MMJJ_RS02235 at 424.006 kb on - strand, within MMJJ_RS02235 at 424.022 kb on - strand, within MMJJ_RS02235 at 424.022 kb on - strand, within MMJJ_RS02235 at 424.022 kb on - strand, within MMJJ_RS02235 at 424.022 kb on - strand, within MMJJ_RS02235 at 424.026 kb on - strand, within MMJJ_RS02235 at 424.026 kb on - strand, within MMJJ_RS02235 at 424.026 kb on - strand, within MMJJ_RS02235 at 424.027 kb on + strand, within MMJJ_RS02235 at 424.027 kb on + strand, within MMJJ_RS02235 at 424.027 kb on + strand, within MMJJ_RS02235 at 424.027 kb on + strand, within MMJJ_RS02235 at 424.030 kb on - strand, within MMJJ_RS02235 at 424.031 kb on + strand, within MMJJ_RS02235 at 424.063 kb on - strand, within MMJJ_RS02235 at 424.194 kb on + strand, within MMJJ_RS02235 at 424.204 kb on + strand, within MMJJ_RS02235 at 424.204 kb on + strand, within MMJJ_RS02235 at 424.259 kb on + strand, within MMJJ_RS02235 at 424.279 kb on - strand, within MMJJ_RS02235 at 424.279 kb on - strand, within MMJJ_RS02235 at 424.283 kb on - strand, within MMJJ_RS02235 at 424.283 kb on - strand, within MMJJ_RS02235 at 424.283 kb on - strand, within MMJJ_RS02235 at 424.283 kb on - strand, within MMJJ_RS02235 at 424.283 kb on - strand, within MMJJ_RS02235 at 424.284 kb on + strand, within MMJJ_RS02235 at 424.284 kb on + strand, within MMJJ_RS02235 at 424.390 kb on - strand, within MMJJ_RS02235 at 424.393 kb on - strand, within MMJJ_RS02235 at 424.394 kb on + strand, within MMJJ_RS02235 at 424.404 kb on + strand, within MMJJ_RS02235 at 424.482 kb on - strand, within MMJJ_RS02235 at 424.491 kb on - strand, within MMJJ_RS02235 at 424.492 kb on + strand, within MMJJ_RS02235 at 424.497 kb on - strand, within MMJJ_RS02235 at 424.543 kb on + strand at 424.557 kb on - strand at 424.558 kb on + strand at 424.558 kb on + strand at 424.558 kb on + strand at 424.589 kb on + strand at 424.599 kb on - strand at 424.599 kb on - strand at 424.600 kb on + strand at 424.601 kb on - strand at 424.604 kb on - strand at 424.604 kb on - strand at 424.605 kb on + strand at 424.605 kb on + strand at 424.610 kb on + strand at 424.630 kb on - strand at 424.631 kb on + strand at 424.631 kb on + strand at 424.655 kb on - strand at 424.655 kb on - strand at 424.655 kb on - strand at 424.655 kb on - strand at 424.656 kb on + strand at 424.659 kb on - strand at 424.659 kb on - strand at 424.659 kb on - strand at 424.659 kb on - strand at 424.659 kb on - strand at 424.660 kb on + strand at 424.660 kb on + strand at 424.660 kb on + strand at 424.663 kb on - strand at 424.664 kb on + strand at 424.664 kb on + strand at 424.666 kb on + strand at 424.666 kb on + strand at 424.666 kb on + strand at 424.769 kb on - strand, within MMJJ_RS02240 at 424.771 kb on - strand, within MMJJ_RS02240 at 424.771 kb on - strand, within MMJJ_RS02240 at 424.772 kb on + strand, within MMJJ_RS02240 at 424.780 kb on - strand, within MMJJ_RS02240 at 424.810 kb on + strand, within MMJJ_RS02240 at 424.898 kb on + strand, within MMJJ_RS02240 at 424.920 kb on + strand, within MMJJ_RS02240 at 424.948 kb on + strand, within MMJJ_RS02240 at 424.960 kb on + strand, within MMJJ_RS02240 at 425.098 kb on - strand at 425.098 kb on - strand at 425.098 kb on - strand at 425.196 kb on - strand at 425.196 kb on - strand at 425.197 kb on + strand at 425.197 kb on + strand at 425.198 kb on + strand at 425.198 kb on - strand at 425.198 kb on - strand at 425.199 kb on + strand at 425.199 kb on + strand at 425.199 kb on + strand at 425.199 kb on + strand at 425.200 kb on - strand at 425.200 kb on - strand at 425.200 kb on - strand at 425.201 kb on + strand at 425.201 kb on + strand at 425.201 kb on + strand at 425.201 kb on + strand at 425.201 kb on + strand at 425.203 kb on - strand at 425.206 kb on - strand at 425.226 kb on - strand at 425.286 kb on - strand, within MMJJ_RS02245 at 425.294 kb on - strand, within MMJJ_RS02245 at 425.295 kb on + strand, within MMJJ_RS02245 at 425.319 kb on - strand, within MMJJ_RS02245 at 425.388 kb on - strand, within MMJJ_RS02245 at 425.414 kb on + strand, within MMJJ_RS02245 at 425.493 kb on - strand, within MMJJ_RS02245 at 425.494 kb on + strand, within MMJJ_RS02245 at 425.494 kb on + strand, within MMJJ_RS02245 at 425.500 kb on + strand, within MMJJ_RS02245 at 425.522 kb on - strand, within MMJJ_RS02245 at 425.523 kb on + strand, within MMJJ_RS02245 at 425.524 kb on - strand, within MMJJ_RS02245 at 425.547 kb on - strand, within MMJJ_RS02245 at 425.548 kb on + strand, within MMJJ_RS02245 at 425.564 kb on - strand, within MMJJ_RS02245 at 425.565 kb on + strand, within MMJJ_RS02245 at 425.580 kb on - strand, within MMJJ_RS02245 at 425.580 kb on - strand, within MMJJ_RS02245 at 425.580 kb on - strand, within MMJJ_RS02245 at 425.581 kb on + strand, within MMJJ_RS02245 at 425.581 kb on + strand, within MMJJ_RS02245 at 425.581 kb on + strand, within MMJJ_RS02245 at 425.581 kb on + strand, within MMJJ_RS02245 at 425.581 kb on + strand, within MMJJ_RS02245 at 425.583 kb on + strand, within MMJJ_RS02245 at 425.584 kb on - strand, within MMJJ_RS02245 at 425.584 kb on - strand, within MMJJ_RS02245 at 425.584 kb on - strand, within MMJJ_RS02245 at 425.585 kb on + strand, within MMJJ_RS02245 at 425.585 kb on + strand, within MMJJ_RS02245 at 425.587 kb on - strand, within MMJJ_RS02245 at 425.587 kb on - strand, within MMJJ_RS02245 at 425.587 kb on - strand, within MMJJ_RS02245 at 425.588 kb on + strand, within MMJJ_RS02245 at 425.626 kb on - strand, within MMJJ_RS02245 at 425.688 kb on - strand, within MMJJ_RS02245 at 425.689 kb on + strand, within MMJJ_RS02245 at 425.689 kb on + strand, within MMJJ_RS02245 at 425.693 kb on - strand, within MMJJ_RS02245 at 425.694 kb on + strand, within MMJJ_RS02245 at 425.932 kb on + strand at 426.010 kb on + strand, within MMJJ_RS02250 at 426.048 kb on - strand, within MMJJ_RS02250
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 423,669 - MMJJ_RS02235 0.11 +1.9 423,872 - MMJJ_RS02235 0.30 -0.5 423,874 - MMJJ_RS02235 0.30 -0.4 423,875 + MMJJ_RS02235 0.31 -1.3 423,946 + MMJJ_RS02235 0.37 -0.9 423,946 + MMJJ_RS02235 0.37 +0.2 423,949 + MMJJ_RS02235 0.37 +0.0 423,971 - MMJJ_RS02235 0.39 -0.8 424,006 - MMJJ_RS02235 0.43 +0.1 424,006 - MMJJ_RS02235 0.43 -1.1 424,006 - MMJJ_RS02235 0.43 -0.9 424,022 - MMJJ_RS02235 0.44 +0.6 424,022 - MMJJ_RS02235 0.44 +0.3 424,022 - MMJJ_RS02235 0.44 -0.9 424,022 - MMJJ_RS02235 0.44 -0.3 424,026 - MMJJ_RS02235 0.45 +1.1 424,026 - MMJJ_RS02235 0.45 +1.3 424,026 - MMJJ_RS02235 0.45 +0.7 424,027 + MMJJ_RS02235 0.45 +0.3 424,027 + MMJJ_RS02235 0.45 +0.1 424,027 + MMJJ_RS02235 0.45 +0.7 424,027 + MMJJ_RS02235 0.45 +0.3 424,030 - MMJJ_RS02235 0.45 -1.0 424,031 + MMJJ_RS02235 0.45 +0.4 424,063 - MMJJ_RS02235 0.48 -0.4 424,194 + MMJJ_RS02235 0.60 +0.3 424,204 + MMJJ_RS02235 0.61 -0.7 424,204 + MMJJ_RS02235 0.61 +0.4 424,259 + MMJJ_RS02235 0.66 +0.2 424,279 - MMJJ_RS02235 0.68 +0.7 424,279 - MMJJ_RS02235 0.68 +0.5 424,283 - MMJJ_RS02235 0.69 -0.2 424,283 - MMJJ_RS02235 0.69 +0.1 424,283 - MMJJ_RS02235 0.69 -0.0 424,283 - MMJJ_RS02235 0.69 +0.6 424,283 - MMJJ_RS02235 0.69 +1.2 424,284 + MMJJ_RS02235 0.69 +0.8 424,284 + MMJJ_RS02235 0.69 +0.7 424,390 - MMJJ_RS02235 0.79 +0.3 424,393 - MMJJ_RS02235 0.79 -0.6 424,394 + MMJJ_RS02235 0.79 +0.5 424,404 + MMJJ_RS02235 0.80 -1.7 424,482 - MMJJ_RS02235 0.87 -0.6 424,491 - MMJJ_RS02235 0.88 +0.1 424,492 + MMJJ_RS02235 0.88 +0.3 424,497 - MMJJ_RS02235 0.89 +0.0 424,543 + +1.2 424,557 - -0.3 424,558 + -0.8 424,558 + -0.7 424,558 + -0.8 424,589 + -0.3 424,599 - +0.6 424,599 - +0.5 424,600 + +2.0 424,601 - -0.4 424,604 - +0.9 424,604 - -0.9 424,605 + -1.3 424,605 + +0.9 424,610 + +0.1 424,630 - -0.9 424,631 + +0.6 424,631 + +0.3 424,655 - +0.5 424,655 - +0.1 424,655 - +0.9 424,655 - -1.1 424,656 + +0.7 424,659 - -0.7 424,659 - +0.9 424,659 - -3.4 424,659 - -0.0 424,659 - -0.1 424,660 + -0.2 424,660 + +0.3 424,660 + +0.9 424,663 - +0.2 424,664 + -0.2 424,664 + -0.4 424,666 + +0.0 424,666 + -0.4 424,666 + -0.8 424,769 - MMJJ_RS02240 0.30 +0.8 424,771 - MMJJ_RS02240 0.31 +0.4 424,771 - MMJJ_RS02240 0.31 +0.5 424,772 + MMJJ_RS02240 0.31 +0.0 424,780 - MMJJ_RS02240 0.33 +0.3 424,810 + MMJJ_RS02240 0.40 +1.1 424,898 + MMJJ_RS02240 0.62 +1.0 424,920 + MMJJ_RS02240 0.67 -0.1 424,948 + MMJJ_RS02240 0.74 -0.8 424,960 + MMJJ_RS02240 0.77 +0.5 425,098 - -0.7 425,098 - -0.3 425,098 - -0.7 425,196 - -0.4 425,196 - -1.1 425,197 + +0.2 425,197 + -0.1 425,198 + -0.4 425,198 - +0.1 425,198 - +0.9 425,199 + +1.1 425,199 + -0.5 425,199 + +1.3 425,199 + -0.1 425,200 - -0.0 425,200 - +0.3 425,200 - -0.0 425,201 + -0.4 425,201 + +0.2 425,201 + -0.8 425,201 + -0.5 425,201 + +0.3 425,203 - +0.4 425,206 - +1.0 425,226 - +0.6 425,286 - MMJJ_RS02245 0.12 -0.1 425,294 - MMJJ_RS02245 0.14 -0.5 425,295 + MMJJ_RS02245 0.14 +0.6 425,319 - MMJJ_RS02245 0.18 -0.8 425,388 - MMJJ_RS02245 0.28 +1.1 425,414 + MMJJ_RS02245 0.32 -2.7 425,493 - MMJJ_RS02245 0.45 +0.8 425,494 + MMJJ_RS02245 0.45 +0.3 425,494 + MMJJ_RS02245 0.45 +2.2 425,500 + MMJJ_RS02245 0.46 -0.2 425,522 - MMJJ_RS02245 0.49 -0.5 425,523 + MMJJ_RS02245 0.49 -0.5 425,524 - MMJJ_RS02245 0.50 -0.9 425,547 - MMJJ_RS02245 0.53 +1.0 425,548 + MMJJ_RS02245 0.53 +0.7 425,564 - MMJJ_RS02245 0.56 -0.5 425,565 + MMJJ_RS02245 0.56 -0.2 425,580 - MMJJ_RS02245 0.58 +0.3 425,580 - MMJJ_RS02245 0.58 -0.2 425,580 - MMJJ_RS02245 0.58 -0.3 425,581 + MMJJ_RS02245 0.59 -1.2 425,581 + MMJJ_RS02245 0.59 -0.1 425,581 + MMJJ_RS02245 0.59 +0.4 425,581 + MMJJ_RS02245 0.59 -0.2 425,581 + MMJJ_RS02245 0.59 -2.3 425,583 + MMJJ_RS02245 0.59 +0.7 425,584 - MMJJ_RS02245 0.59 +0.5 425,584 - MMJJ_RS02245 0.59 -0.8 425,584 - MMJJ_RS02245 0.59 -1.9 425,585 + MMJJ_RS02245 0.59 +0.2 425,585 + MMJJ_RS02245 0.59 -2.2 425,587 - MMJJ_RS02245 0.59 -0.4 425,587 - MMJJ_RS02245 0.59 +0.9 425,587 - MMJJ_RS02245 0.59 -0.8 425,588 + MMJJ_RS02245 0.60 -1.2 425,626 - MMJJ_RS02245 0.66 +0.6 425,688 - MMJJ_RS02245 0.75 -0.6 425,689 + MMJJ_RS02245 0.75 +1.4 425,689 + MMJJ_RS02245 0.75 -1.3 425,693 - MMJJ_RS02245 0.76 -0.2 425,694 + MMJJ_RS02245 0.76 +0.2 425,932 + -0.6 426,010 + MMJJ_RS02250 0.15 +0.3 426,048 - MMJJ_RS02250 0.19 +1.1
Or see this region's nucleotide sequence