Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01660 and MMJJ_RS01665 are separated by 3 nucleotides MMJJ_RS01665 and MMJJ_RS01670 are separated by 1 nucleotides MMJJ_RS01670 and MMJJ_RS01675 are separated by 213 nucleotides
MMJJ_RS01660: MMJJ_RS01660 - RNA 3'-terminal phosphate cyclase, at 322,809 to 323,828
_RS01660
MMJJ_RS01665: MMJJ_RS01665 - archease, at 323,832 to 324,248
_RS01665
MMJJ_RS01670: MMJJ_RS01670 - hypothetical protein, at 324,250 to 324,450
_RS01670
MMJJ_RS01675: MMJJ_RS01675 - malate dehydrogenase, at 324,664 to 325,608
_RS01675
Position (kb) 324
325 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 323.332 kb on - strand, within MMJJ_RS01660 at 323.347 kb on - strand, within MMJJ_RS01660 at 323.347 kb on - strand, within MMJJ_RS01660 at 323.408 kb on - strand, within MMJJ_RS01660 at 323.409 kb on + strand, within MMJJ_RS01660 at 323.409 kb on + strand, within MMJJ_RS01660 at 323.446 kb on - strand, within MMJJ_RS01660 at 323.446 kb on - strand, within MMJJ_RS01660 at 323.446 kb on - strand, within MMJJ_RS01660 at 323.447 kb on + strand, within MMJJ_RS01660 at 323.450 kb on + strand, within MMJJ_RS01660 at 323.474 kb on - strand, within MMJJ_RS01660 at 323.475 kb on + strand, within MMJJ_RS01660 at 323.516 kb on + strand, within MMJJ_RS01660 at 323.524 kb on + strand, within MMJJ_RS01660 at 323.525 kb on - strand, within MMJJ_RS01660 at 323.525 kb on - strand, within MMJJ_RS01660 at 323.532 kb on - strand, within MMJJ_RS01660 at 323.532 kb on - strand, within MMJJ_RS01660 at 323.533 kb on + strand, within MMJJ_RS01660 at 323.533 kb on + strand, within MMJJ_RS01660 at 323.533 kb on + strand, within MMJJ_RS01660 at 323.730 kb on + strand at 323.759 kb on - strand at 323.760 kb on + strand at 323.760 kb on + strand at 323.764 kb on + strand at 323.765 kb on - strand at 323.766 kb on + strand at 323.768 kb on - strand at 323.806 kb on - strand at 323.806 kb on - strand at 323.806 kb on - strand at 323.807 kb on + strand at 323.807 kb on + strand at 323.809 kb on - strand at 323.827 kb on + strand at 323.835 kb on - strand at 323.835 kb on - strand at 323.835 kb on - strand at 323.837 kb on - strand at 323.838 kb on + strand at 323.842 kb on + strand at 323.842 kb on + strand at 323.870 kb on + strand at 323.985 kb on + strand, within MMJJ_RS01665 at 324.010 kb on - strand, within MMJJ_RS01665 at 324.010 kb on - strand, within MMJJ_RS01665 at 324.010 kb on - strand, within MMJJ_RS01665 at 324.011 kb on + strand, within MMJJ_RS01665 at 324.011 kb on + strand, within MMJJ_RS01665 at 324.011 kb on + strand, within MMJJ_RS01665 at 324.011 kb on + strand, within MMJJ_RS01665 at 324.013 kb on + strand, within MMJJ_RS01665 at 324.058 kb on - strand, within MMJJ_RS01665 at 324.061 kb on - strand, within MMJJ_RS01665 at 324.062 kb on + strand, within MMJJ_RS01665 at 324.062 kb on + strand, within MMJJ_RS01665 at 324.109 kb on - strand, within MMJJ_RS01665 at 324.109 kb on - strand, within MMJJ_RS01665 at 324.110 kb on + strand, within MMJJ_RS01665 at 324.110 kb on + strand, within MMJJ_RS01665 at 324.131 kb on - strand, within MMJJ_RS01665 at 324.132 kb on + strand at 324.151 kb on + strand, within MMJJ_RS01665 at 324.151 kb on + strand, within MMJJ_RS01665 at 324.151 kb on + strand, within MMJJ_RS01665 at 324.169 kb on - strand, within MMJJ_RS01665 at 324.180 kb on + strand, within MMJJ_RS01665 at 324.183 kb on - strand, within MMJJ_RS01665 at 324.183 kb on - strand, within MMJJ_RS01665 at 324.239 kb on + strand at 324.251 kb on - strand at 324.252 kb on + strand at 324.252 kb on + strand at 324.268 kb on + strand at 324.272 kb on - strand, within MMJJ_RS01670 at 324.272 kb on - strand, within MMJJ_RS01670 at 324.272 kb on - strand, within MMJJ_RS01670 at 324.272 kb on - strand, within MMJJ_RS01670 at 324.272 kb on - strand, within MMJJ_RS01670 at 324.273 kb on + strand, within MMJJ_RS01670 at 324.273 kb on + strand, within MMJJ_RS01670 at 324.279 kb on - strand, within MMJJ_RS01670 at 324.279 kb on - strand, within MMJJ_RS01670 at 324.280 kb on + strand, within MMJJ_RS01670 at 324.301 kb on + strand, within MMJJ_RS01670 at 324.352 kb on + strand, within MMJJ_RS01670 at 324.397 kb on - strand, within MMJJ_RS01670 at 324.450 kb on - strand at 324.450 kb on - strand at 324.450 kb on - strand at 324.451 kb on + strand at 324.482 kb on - strand at 324.482 kb on - strand at 324.482 kb on - strand at 324.483 kb on + strand at 324.516 kb on - strand at 324.517 kb on + strand at 324.544 kb on + strand at 324.562 kb on - strand at 324.562 kb on - strand at 324.563 kb on + strand at 324.567 kb on + strand at 324.606 kb on - strand at 324.607 kb on + strand at 324.610 kb on - strand at 324.610 kb on - strand at 324.610 kb on - strand at 324.610 kb on - strand at 324.610 kb on - strand at 324.611 kb on + strand at 324.611 kb on + strand at 324.611 kb on + strand at 324.612 kb on - strand at 324.612 kb on - strand at 324.612 kb on - strand at 324.612 kb on - strand at 324.612 kb on - strand at 324.612 kb on - strand at 324.612 kb on - strand at 324.612 kb on - strand at 324.612 kb on - strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.613 kb on + strand at 324.614 kb on - strand at 324.614 kb on - strand at 324.614 kb on - strand at 324.614 kb on - strand at 324.614 kb on - strand at 324.615 kb on + strand at 324.615 kb on + strand at 324.615 kb on + strand at 324.615 kb on + strand at 324.615 kb on + strand at 324.618 kb on - strand at 324.618 kb on - strand at 324.619 kb on + strand at 324.619 kb on + strand at 324.620 kb on - strand at 324.620 kb on - strand at 324.633 kb on + strand at 324.633 kb on + strand at 324.633 kb on + strand at 324.645 kb on - strand at 324.646 kb on + strand at 324.736 kb on - strand at 324.780 kb on - strand, within MMJJ_RS01675 at 324.808 kb on - strand, within MMJJ_RS01675 at 324.837 kb on - strand, within MMJJ_RS01675 at 324.840 kb on - strand, within MMJJ_RS01675 at 324.841 kb on + strand, within MMJJ_RS01675 at 324.841 kb on + strand, within MMJJ_RS01675 at 324.851 kb on - strand, within MMJJ_RS01675 at 324.851 kb on - strand, within MMJJ_RS01675 at 324.851 kb on - strand, within MMJJ_RS01675 at 324.852 kb on + strand, within MMJJ_RS01675 at 324.852 kb on + strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.869 kb on - strand, within MMJJ_RS01675 at 324.870 kb on + strand, within MMJJ_RS01675 at 324.876 kb on - strand, within MMJJ_RS01675 at 324.876 kb on - strand, within MMJJ_RS01675 at 324.876 kb on - strand, within MMJJ_RS01675 at 324.876 kb on - strand, within MMJJ_RS01675 at 324.877 kb on + strand, within MMJJ_RS01675 at 324.877 kb on + strand, within MMJJ_RS01675 at 324.877 kb on + strand, within MMJJ_RS01675 at 324.882 kb on - strand, within MMJJ_RS01675 at 324.891 kb on - strand, within MMJJ_RS01675 at 324.891 kb on - strand, within MMJJ_RS01675 at 324.892 kb on + strand, within MMJJ_RS01675 at 324.892 kb on + strand, within MMJJ_RS01675 at 324.899 kb on - strand, within MMJJ_RS01675 at 324.899 kb on - strand, within MMJJ_RS01675 at 324.904 kb on + strand, within MMJJ_RS01675 at 324.904 kb on + strand, within MMJJ_RS01675 at 324.948 kb on - strand, within MMJJ_RS01675 at 324.990 kb on - strand, within MMJJ_RS01675 at 324.990 kb on - strand, within MMJJ_RS01675 at 324.991 kb on + strand at 324.991 kb on + strand, within MMJJ_RS01675 at 324.996 kb on - strand at 324.996 kb on - strand, within MMJJ_RS01675 at 324.997 kb on + strand, within MMJJ_RS01675 at 324.997 kb on + strand, within MMJJ_RS01675 at 325.011 kb on - strand, within MMJJ_RS01675 at 325.011 kb on - strand, within MMJJ_RS01675 at 325.011 kb on - strand, within MMJJ_RS01675 at 325.012 kb on + strand, within MMJJ_RS01675 at 325.017 kb on + strand, within MMJJ_RS01675 at 325.051 kb on - strand, within MMJJ_RS01675 at 325.051 kb on - strand, within MMJJ_RS01675 at 325.051 kb on - strand, within MMJJ_RS01675 at 325.051 kb on - strand at 325.051 kb on - strand, within MMJJ_RS01675 at 325.051 kb on - strand, within MMJJ_RS01675 at 325.051 kb on - strand, within MMJJ_RS01675 at 325.051 kb on - strand, within MMJJ_RS01675 at 325.052 kb on + strand, within MMJJ_RS01675 at 325.052 kb on + strand, within MMJJ_RS01675 at 325.052 kb on + strand, within MMJJ_RS01675 at 325.075 kb on - strand, within MMJJ_RS01675 at 325.128 kb on - strand, within MMJJ_RS01675 at 325.134 kb on + strand, within MMJJ_RS01675 at 325.134 kb on + strand, within MMJJ_RS01675 at 325.174 kb on + strand, within MMJJ_RS01675 at 325.174 kb on + strand, within MMJJ_RS01675 at 325.235 kb on - strand, within MMJJ_RS01675 at 325.236 kb on + strand, within MMJJ_RS01675 at 325.236 kb on + strand, within MMJJ_RS01675 at 325.267 kb on - strand, within MMJJ_RS01675 at 325.305 kb on - strand, within MMJJ_RS01675 at 325.306 kb on + strand, within MMJJ_RS01675 at 325.312 kb on - strand, within MMJJ_RS01675 at 325.329 kb on + strand, within MMJJ_RS01675 at 325.329 kb on + strand, within MMJJ_RS01675 at 325.373 kb on - strand, within MMJJ_RS01675 at 325.382 kb on - strand, within MMJJ_RS01675 at 325.382 kb on - strand, within MMJJ_RS01675 at 325.383 kb on + strand, within MMJJ_RS01675 at 325.383 kb on + strand, within MMJJ_RS01675 at 325.383 kb on + strand, within MMJJ_RS01675 at 325.383 kb on + strand at 325.383 kb on + strand, within MMJJ_RS01675 at 325.383 kb on + strand, within MMJJ_RS01675 at 325.389 kb on - strand, within MMJJ_RS01675 at 325.389 kb on - strand, within MMJJ_RS01675 at 325.390 kb on + strand, within MMJJ_RS01675
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 323,332 - MMJJ_RS01660 0.51 -2.2 323,347 - MMJJ_RS01660 0.53 -0.8 323,347 - MMJJ_RS01660 0.53 +0.2 323,408 - MMJJ_RS01660 0.59 +1.0 323,409 + MMJJ_RS01660 0.59 -1.8 323,409 + MMJJ_RS01660 0.59 -0.2 323,446 - MMJJ_RS01660 0.62 +0.8 323,446 - MMJJ_RS01660 0.62 +0.9 323,446 - MMJJ_RS01660 0.62 -0.6 323,447 + MMJJ_RS01660 0.63 -0.6 323,450 + MMJJ_RS01660 0.63 +0.4 323,474 - MMJJ_RS01660 0.65 -0.1 323,475 + MMJJ_RS01660 0.65 -0.4 323,516 + MMJJ_RS01660 0.69 -1.9 323,524 + MMJJ_RS01660 0.70 +0.6 323,525 - MMJJ_RS01660 0.70 +1.1 323,525 - MMJJ_RS01660 0.70 -2.6 323,532 - MMJJ_RS01660 0.71 -1.0 323,532 - MMJJ_RS01660 0.71 +1.1 323,533 + MMJJ_RS01660 0.71 +1.0 323,533 + MMJJ_RS01660 0.71 -0.5 323,533 + MMJJ_RS01660 0.71 +1.1 323,730 + +0.5 323,759 - -0.2 323,760 + -1.6 323,760 + +0.2 323,764 + +2.1 323,765 - -0.2 323,766 + +0.3 323,768 - +0.9 323,806 - +0.8 323,806 - +0.2 323,806 - -1.0 323,807 + +1.0 323,807 + +0.2 323,809 - +1.1 323,827 + -0.6 323,835 - +1.1 323,835 - +1.3 323,835 - +0.3 323,837 - +0.2 323,838 + +0.6 323,842 + +0.3 323,842 + +0.3 323,870 + -1.8 323,985 + MMJJ_RS01665 0.37 +0.4 324,010 - MMJJ_RS01665 0.43 +0.4 324,010 - MMJJ_RS01665 0.43 +0.7 324,010 - MMJJ_RS01665 0.43 +0.3 324,011 + MMJJ_RS01665 0.43 +1.5 324,011 + MMJJ_RS01665 0.43 +0.1 324,011 + MMJJ_RS01665 0.43 -0.3 324,011 + MMJJ_RS01665 0.43 +0.8 324,013 + MMJJ_RS01665 0.43 -0.2 324,058 - MMJJ_RS01665 0.54 +0.6 324,061 - MMJJ_RS01665 0.55 +0.2 324,062 + MMJJ_RS01665 0.55 +0.4 324,062 + MMJJ_RS01665 0.55 -0.5 324,109 - MMJJ_RS01665 0.66 +0.8 324,109 - MMJJ_RS01665 0.66 -3.0 324,110 + MMJJ_RS01665 0.67 +1.5 324,110 + MMJJ_RS01665 0.67 +1.4 324,131 - MMJJ_RS01665 0.72 -0.8 324,132 + +1.2 324,151 + MMJJ_RS01665 0.76 -2.4 324,151 + MMJJ_RS01665 0.76 +0.9 324,151 + MMJJ_RS01665 0.76 +0.6 324,169 - MMJJ_RS01665 0.81 +0.5 324,180 + MMJJ_RS01665 0.83 +0.1 324,183 - MMJJ_RS01665 0.84 -1.2 324,183 - MMJJ_RS01665 0.84 +1.1 324,239 + +0.6 324,251 - +2.1 324,252 + +0.8 324,252 + -2.2 324,268 + -1.8 324,272 - MMJJ_RS01670 0.11 +1.0 324,272 - MMJJ_RS01670 0.11 +1.2 324,272 - MMJJ_RS01670 0.11 +0.2 324,272 - MMJJ_RS01670 0.11 -0.6 324,272 - MMJJ_RS01670 0.11 -0.5 324,273 + MMJJ_RS01670 0.11 +1.4 324,273 + MMJJ_RS01670 0.11 -0.8 324,279 - MMJJ_RS01670 0.14 +0.5 324,279 - MMJJ_RS01670 0.14 -0.6 324,280 + MMJJ_RS01670 0.15 -0.4 324,301 + MMJJ_RS01670 0.25 -2.0 324,352 + MMJJ_RS01670 0.51 +0.4 324,397 - MMJJ_RS01670 0.73 +1.0 324,450 - -0.8 324,450 - -0.8 324,450 - -1.6 324,451 + -2.0 324,482 - +0.3 324,482 - -1.4 324,482 - +0.4 324,483 + -0.5 324,516 - -2.2 324,517 + +0.3 324,544 + -0.8 324,562 - -1.1 324,562 - -0.5 324,563 + -0.3 324,567 + +0.8 324,606 - -1.1 324,607 + +0.9 324,610 - +1.2 324,610 - -0.4 324,610 - -0.4 324,610 - -0.2 324,610 - +0.1 324,611 + +0.7 324,611 + +0.2 324,611 + -1.5 324,612 - +0.8 324,612 - -1.4 324,612 - +0.9 324,612 - +0.4 324,612 - +1.4 324,612 - +1.0 324,612 - +0.1 324,612 - -2.2 324,612 - -2.4 324,613 + +0.5 324,613 + +1.7 324,613 + +0.4 324,613 + -2.2 324,613 + +0.8 324,613 + +0.3 324,613 + +0.3 324,613 + -0.4 324,613 + +0.6 324,613 + +0.7 324,613 + +0.3 324,613 + +0.4 324,613 + +0.5 324,613 + +0.8 324,613 + -2.2 324,614 - -1.8 324,614 - +0.6 324,614 - -0.1 324,614 - +2.6 324,614 - +0.4 324,615 + +0.2 324,615 + +0.5 324,615 + -3.2 324,615 + +1.8 324,615 + +0.2 324,618 - +1.7 324,618 - -1.5 324,619 + +0.3 324,619 + -0.3 324,620 - +0.8 324,620 - -0.2 324,633 + +0.1 324,633 + +1.3 324,633 + -1.0 324,645 - +0.4 324,646 + +0.2 324,736 - -1.0 324,780 - MMJJ_RS01675 0.12 +1.0 324,808 - MMJJ_RS01675 0.15 +0.8 324,837 - MMJJ_RS01675 0.18 +0.3 324,840 - MMJJ_RS01675 0.19 +1.0 324,841 + MMJJ_RS01675 0.19 +0.8 324,841 + MMJJ_RS01675 0.19 +0.3 324,851 - MMJJ_RS01675 0.20 +1.0 324,851 - MMJJ_RS01675 0.20 +0.4 324,851 - MMJJ_RS01675 0.20 +1.8 324,852 + MMJJ_RS01675 0.20 +0.2 324,852 + MMJJ_RS01675 0.20 +1.0 324,869 - MMJJ_RS01675 0.22 -0.1 324,869 - MMJJ_RS01675 0.22 +0.7 324,869 - MMJJ_RS01675 0.22 +2.5 324,869 - MMJJ_RS01675 0.22 +0.6 324,869 - MMJJ_RS01675 0.22 +1.0 324,869 - MMJJ_RS01675 0.22 +0.6 324,869 - MMJJ_RS01675 0.22 +0.6 324,870 + MMJJ_RS01675 0.22 +0.3 324,876 - MMJJ_RS01675 0.22 +0.3 324,876 - MMJJ_RS01675 0.22 -1.4 324,876 - MMJJ_RS01675 0.22 -0.9 324,876 - MMJJ_RS01675 0.22 -1.2 324,877 + MMJJ_RS01675 0.23 +1.2 324,877 + MMJJ_RS01675 0.23 -0.0 324,877 + MMJJ_RS01675 0.23 +1.1 324,882 - MMJJ_RS01675 0.23 -1.2 324,891 - MMJJ_RS01675 0.24 -0.2 324,891 - MMJJ_RS01675 0.24 +1.0 324,892 + MMJJ_RS01675 0.24 +0.2 324,892 + MMJJ_RS01675 0.24 +0.3 324,899 - MMJJ_RS01675 0.25 +0.1 324,899 - MMJJ_RS01675 0.25 +1.0 324,904 + MMJJ_RS01675 0.25 +0.0 324,904 + MMJJ_RS01675 0.25 +1.2 324,948 - MMJJ_RS01675 0.30 +0.7 324,990 - MMJJ_RS01675 0.34 -0.8 324,990 - MMJJ_RS01675 0.34 +0.2 324,991 + -0.8 324,991 + MMJJ_RS01675 0.35 -0.4 324,996 - +2.2 324,996 - MMJJ_RS01675 0.35 +0.2 324,997 + MMJJ_RS01675 0.35 +0.8 324,997 + MMJJ_RS01675 0.35 +0.2 325,011 - MMJJ_RS01675 0.37 +1.4 325,011 - MMJJ_RS01675 0.37 +0.4 325,011 - MMJJ_RS01675 0.37 +1.2 325,012 + MMJJ_RS01675 0.37 +0.4 325,017 + MMJJ_RS01675 0.37 +0.5 325,051 - MMJJ_RS01675 0.41 +0.2 325,051 - MMJJ_RS01675 0.41 +1.5 325,051 - MMJJ_RS01675 0.41 +1.2 325,051 - -0.4 325,051 - MMJJ_RS01675 0.41 +0.8 325,051 - MMJJ_RS01675 0.41 -2.5 325,051 - MMJJ_RS01675 0.41 +0.0 325,051 - MMJJ_RS01675 0.41 +0.0 325,052 + MMJJ_RS01675 0.41 -1.8 325,052 + MMJJ_RS01675 0.41 +0.4 325,052 + MMJJ_RS01675 0.41 +0.3 325,075 - MMJJ_RS01675 0.43 +0.4 325,128 - MMJJ_RS01675 0.49 -4.0 325,134 + MMJJ_RS01675 0.50 +0.5 325,134 + MMJJ_RS01675 0.50 +0.5 325,174 + MMJJ_RS01675 0.54 +0.4 325,174 + MMJJ_RS01675 0.54 +0.5 325,235 - MMJJ_RS01675 0.60 -0.5 325,236 + MMJJ_RS01675 0.61 +0.9 325,236 + MMJJ_RS01675 0.61 +0.8 325,267 - MMJJ_RS01675 0.64 +0.9 325,305 - MMJJ_RS01675 0.68 -0.3 325,306 + MMJJ_RS01675 0.68 +0.6 325,312 - MMJJ_RS01675 0.69 +1.8 325,329 + MMJJ_RS01675 0.70 +0.7 325,329 + MMJJ_RS01675 0.70 +0.8 325,373 - MMJJ_RS01675 0.75 -2.1 325,382 - MMJJ_RS01675 0.76 +0.5 325,382 - MMJJ_RS01675 0.76 +0.4 325,383 + MMJJ_RS01675 0.76 -0.8 325,383 + MMJJ_RS01675 0.76 -0.2 325,383 + MMJJ_RS01675 0.76 -0.4 325,383 + +1.2 325,383 + MMJJ_RS01675 0.76 +0.9 325,383 + MMJJ_RS01675 0.76 +0.2 325,389 - MMJJ_RS01675 0.77 -0.4 325,389 - MMJJ_RS01675 0.77 -1.1 325,390 + MMJJ_RS01675 0.77 +0.3
Or see this region's nucleotide sequence