Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01105 and MMJJ_RS01110 are separated by 743 nucleotides MMJJ_RS01110 and MMJJ_RS01115 are separated by 423 nucleotides MMJJ_RS01115 and MMJJ_RS01120 are separated by 199 nucleotides
MMJJ_RS01105: MMJJ_RS01105 - DUF5906 domain-containing protein, at 213,028 to 215,544
_RS01105
MMJJ_RS01110: MMJJ_RS01110 - hypothetical protein, at 216,288 to 216,590
_RS01110
MMJJ_RS01115: MMJJ_RS01115 - hypothetical protein, at 217,014 to 217,340
_RS01115
MMJJ_RS01120: MMJJ_RS01120 - hypothetical protein, at 217,540 to 217,821
_RS01120
Position (kb) 216
217 Strain fitness (log2 ratio) -2
-1
0
1
2 at 215.312 kb on - strand at 215.314 kb on - strand at 215.318 kb on - strand at 215.318 kb on - strand at 215.446 kb on - strand at 215.448 kb on - strand at 215.449 kb on + strand at 215.452 kb on - strand at 215.453 kb on + strand at 215.480 kb on - strand at 215.480 kb on - strand at 215.480 kb on - strand at 215.480 kb on - strand at 215.480 kb on - strand at 215.480 kb on - strand at 215.481 kb on + strand at 215.481 kb on + strand at 215.481 kb on + strand at 215.481 kb on + strand at 215.481 kb on + strand at 215.548 kb on - strand at 215.549 kb on + strand at 215.549 kb on + strand at 215.638 kb on - strand at 215.639 kb on + strand at 215.644 kb on - strand at 215.666 kb on - strand at 215.720 kb on + strand at 215.780 kb on - strand at 215.780 kb on - strand at 215.782 kb on - strand at 215.782 kb on - strand at 215.783 kb on + strand at 215.784 kb on - strand at 215.785 kb on + strand at 215.785 kb on + strand at 215.785 kb on + strand at 215.835 kb on - strand at 215.836 kb on + strand at 215.836 kb on + strand at 215.842 kb on - strand at 215.921 kb on + strand at 216.087 kb on - strand at 216.135 kb on - strand at 216.136 kb on + strand at 216.140 kb on + strand at 216.140 kb on + strand at 216.142 kb on + strand at 216.144 kb on - strand at 216.144 kb on - strand at 216.145 kb on + strand at 216.145 kb on + strand at 216.148 kb on + strand at 216.149 kb on - strand at 216.149 kb on - strand at 216.247 kb on - strand at 216.247 kb on - strand at 216.247 kb on - strand at 216.247 kb on - strand at 216.286 kb on - strand at 216.287 kb on + strand at 216.293 kb on + strand at 216.298 kb on - strand at 216.333 kb on + strand, within MMJJ_RS01110 at 216.378 kb on + strand, within MMJJ_RS01110 at 216.391 kb on + strand, within MMJJ_RS01110 at 216.418 kb on - strand, within MMJJ_RS01110 at 216.419 kb on + strand, within MMJJ_RS01110 at 216.419 kb on + strand, within MMJJ_RS01110 at 216.506 kb on - strand, within MMJJ_RS01110 at 216.524 kb on + strand, within MMJJ_RS01110 at 216.529 kb on - strand, within MMJJ_RS01110 at 216.639 kb on + strand at 216.652 kb on + strand at 216.672 kb on + strand at 216.712 kb on - strand at 216.712 kb on - strand at 216.712 kb on - strand at 216.807 kb on + strand at 216.809 kb on - strand at 216.866 kb on + strand at 216.960 kb on - strand at 216.960 kb on - strand at 216.961 kb on + strand at 216.962 kb on - strand at 217.121 kb on - strand, within MMJJ_RS01115 at 217.213 kb on - strand, within MMJJ_RS01115 at 217.215 kb on - strand, within MMJJ_RS01115 at 217.215 kb on - strand, within MMJJ_RS01115 at 217.215 kb on - strand, within MMJJ_RS01115 at 217.216 kb on + strand, within MMJJ_RS01115 at 217.270 kb on - strand, within MMJJ_RS01115 at 217.281 kb on - strand, within MMJJ_RS01115 at 217.281 kb on - strand, within MMJJ_RS01115 at 217.282 kb on + strand, within MMJJ_RS01115 at 217.282 kb on + strand, within MMJJ_RS01115 at 217.283 kb on - strand, within MMJJ_RS01115 at 217.283 kb on - strand, within MMJJ_RS01115 at 217.335 kb on + strand at 217.469 kb on + strand at 217.469 kb on + strand at 217.469 kb on + strand at 217.472 kb on + strand at 217.472 kb on + strand at 217.474 kb on - strand at 217.480 kb on - strand at 217.480 kb on - strand at 217.480 kb on - strand at 217.481 kb on + strand at 217.481 kb on + strand at 217.482 kb on - strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.483 kb on + strand at 217.484 kb on - strand at 217.484 kb on - strand at 217.484 kb on - strand at 217.484 kb on - strand at 217.484 kb on - strand at 217.485 kb on + strand at 217.485 kb on + strand at 217.485 kb on + strand at 217.485 kb on + strand at 217.485 kb on + strand at 217.486 kb on - strand at 217.486 kb on - strand at 217.486 kb on - strand at 217.486 kb on - strand at 217.486 kb on - strand at 217.487 kb on + strand at 217.487 kb on + strand at 217.487 kb on + strand at 217.487 kb on + strand at 217.578 kb on - strand, within MMJJ_RS01120 at 217.579 kb on + strand, within MMJJ_RS01120 at 217.580 kb on - strand, within MMJJ_RS01120 at 217.581 kb on + strand, within MMJJ_RS01120
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 215,312 - +1.1 215,314 - +1.0 215,318 - +0.8 215,318 - -0.0 215,446 - +0.6 215,448 - +0.8 215,449 + -0.2 215,452 - -0.9 215,453 + -0.2 215,480 - -0.3 215,480 - -0.1 215,480 - +0.3 215,480 - +0.2 215,480 - +1.3 215,480 - +0.1 215,481 + +0.9 215,481 + -0.8 215,481 + +0.6 215,481 + -0.5 215,481 + +0.6 215,548 - +0.3 215,549 + +0.7 215,549 + -1.4 215,638 - -0.5 215,639 + +0.7 215,644 - +1.5 215,666 - +0.6 215,720 + +0.4 215,780 - -0.1 215,780 - +1.2 215,782 - +0.8 215,782 - +0.2 215,783 + -0.1 215,784 - +0.8 215,785 + -0.9 215,785 + -0.4 215,785 + -0.6 215,835 - -0.5 215,836 + -0.5 215,836 + +0.3 215,842 - +0.4 215,921 + -1.1 216,087 - -1.2 216,135 - +0.9 216,136 + +0.2 216,140 + -0.3 216,140 + +0.1 216,142 + -0.6 216,144 - -0.7 216,144 - +0.1 216,145 + +0.4 216,145 + -0.8 216,148 + +1.8 216,149 - -1.7 216,149 - -0.8 216,247 - +0.1 216,247 - +0.2 216,247 - +0.2 216,247 - +0.1 216,286 - +0.4 216,287 + -1.0 216,293 + +0.5 216,298 - +1.0 216,333 + MMJJ_RS01110 0.15 +0.4 216,378 + MMJJ_RS01110 0.30 +0.6 216,391 + MMJJ_RS01110 0.34 +0.1 216,418 - MMJJ_RS01110 0.43 -0.7 216,419 + MMJJ_RS01110 0.43 +0.5 216,419 + MMJJ_RS01110 0.43 +0.7 216,506 - MMJJ_RS01110 0.72 -0.3 216,524 + MMJJ_RS01110 0.78 +0.5 216,529 - MMJJ_RS01110 0.80 +0.6 216,639 + -0.4 216,652 + +0.5 216,672 + -0.6 216,712 - -1.7 216,712 - +0.0 216,712 - -0.5 216,807 + -2.2 216,809 - +0.1 216,866 + -0.8 216,960 - +0.4 216,960 - +0.6 216,961 + -1.1 216,962 - -1.0 217,121 - MMJJ_RS01115 0.33 -0.8 217,213 - MMJJ_RS01115 0.61 +1.1 217,215 - MMJJ_RS01115 0.61 -0.8 217,215 - MMJJ_RS01115 0.61 +0.8 217,215 - MMJJ_RS01115 0.61 +0.8 217,216 + MMJJ_RS01115 0.62 +0.6 217,270 - MMJJ_RS01115 0.78 +0.3 217,281 - MMJJ_RS01115 0.82 +0.8 217,281 - MMJJ_RS01115 0.82 +0.2 217,282 + MMJJ_RS01115 0.82 +0.5 217,282 + MMJJ_RS01115 0.82 +0.1 217,283 - MMJJ_RS01115 0.82 -0.9 217,283 - MMJJ_RS01115 0.82 +1.6 217,335 + -2.1 217,469 + +0.7 217,469 + +0.4 217,469 + -2.7 217,472 + -0.1 217,472 + -2.1 217,474 - -0.4 217,480 - -0.2 217,480 - -0.9 217,480 - +0.3 217,481 + +1.0 217,481 + -1.5 217,482 - +0.4 217,483 + +0.5 217,483 + -1.2 217,483 + -0.8 217,483 + -0.2 217,483 + -1.2 217,483 + -0.3 217,483 + -0.5 217,483 + +0.6 217,484 - +0.8 217,484 - -0.0 217,484 - +0.1 217,484 - +0.3 217,484 - -2.1 217,485 + +0.2 217,485 + -0.1 217,485 + +0.3 217,485 + -0.6 217,485 + +0.2 217,486 - +1.2 217,486 - +1.2 217,486 - +1.3 217,486 - +1.7 217,486 - +0.2 217,487 + +0.3 217,487 + -0.9 217,487 + -0.6 217,487 + -0.3 217,578 - MMJJ_RS01120 0.13 -0.1 217,579 + MMJJ_RS01120 0.14 +0.9 217,580 - MMJJ_RS01120 0.14 +1.6 217,581 + MMJJ_RS01120 0.15 +1.0
Or see this region's nucleotide sequence