Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00350 and MMJJ_RS00355 are separated by 113 nucleotides MMJJ_RS00355 and MMJJ_RS00360 are separated by 164 nucleotides MMJJ_RS00360 and MMJJ_RS00365 are separated by 31 nucleotides MMJJ_RS00365 and MMJJ_RS00370 are separated by 96 nucleotides
MMJJ_RS00350: MMJJ_RS00350 - phage infection protein, at 67,136 to 67,786
_RS00350
MMJJ_RS00355: MMJJ_RS00355 - ribonuclease P protein component 4, at 67,900 to 68,232
_RS00355
MMJJ_RS00360: MMJJ_RS00360 - adenosylhomocysteinase, at 68,397 to 69,644
_RS00360
MMJJ_RS00365: MMJJ_RS00365 - dihydroorotate dehydrogenase electron transfer subunit, at 69,676 to 70,443
_RS00365
MMJJ_RS00370: MMJJ_RS00370 - asparagine synthase (glutamine-hydrolyzing), at 70,540 to 72,081
_RS00370
Position (kb) 68
69
70 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 67.397 kb on - strand, within MMJJ_RS00350 at 67.397 kb on - strand, within MMJJ_RS00350 at 67.397 kb on - strand, within MMJJ_RS00350 at 67.397 kb on - strand, within MMJJ_RS00350 at 67.398 kb on + strand, within MMJJ_RS00350 at 67.398 kb on + strand, within MMJJ_RS00350 at 67.403 kb on - strand, within MMJJ_RS00350 at 67.404 kb on + strand, within MMJJ_RS00350 at 67.404 kb on + strand at 67.404 kb on + strand, within MMJJ_RS00350 at 67.408 kb on - strand, within MMJJ_RS00350 at 67.408 kb on - strand, within MMJJ_RS00350 at 67.409 kb on + strand, within MMJJ_RS00350 at 67.409 kb on + strand, within MMJJ_RS00350 at 67.433 kb on - strand, within MMJJ_RS00350 at 67.437 kb on + strand, within MMJJ_RS00350 at 67.439 kb on - strand, within MMJJ_RS00350 at 67.439 kb on - strand, within MMJJ_RS00350 at 67.439 kb on - strand, within MMJJ_RS00350 at 67.439 kb on - strand, within MMJJ_RS00350 at 67.439 kb on - strand, within MMJJ_RS00350 at 67.440 kb on + strand, within MMJJ_RS00350 at 67.451 kb on + strand, within MMJJ_RS00350 at 67.451 kb on + strand, within MMJJ_RS00350 at 67.451 kb on + strand, within MMJJ_RS00350 at 67.453 kb on - strand at 67.457 kb on + strand, within MMJJ_RS00350 at 67.474 kb on - strand, within MMJJ_RS00350 at 67.474 kb on - strand, within MMJJ_RS00350 at 67.555 kb on - strand, within MMJJ_RS00350 at 67.556 kb on + strand, within MMJJ_RS00350 at 67.581 kb on - strand, within MMJJ_RS00350 at 67.581 kb on - strand, within MMJJ_RS00350 at 67.581 kb on - strand, within MMJJ_RS00350 at 67.581 kb on - strand, within MMJJ_RS00350 at 67.620 kb on - strand, within MMJJ_RS00350 at 67.670 kb on + strand, within MMJJ_RS00350 at 67.670 kb on + strand, within MMJJ_RS00350 at 67.670 kb on + strand, within MMJJ_RS00350 at 67.671 kb on - strand, within MMJJ_RS00350 at 67.671 kb on - strand, within MMJJ_RS00350 at 67.672 kb on + strand, within MMJJ_RS00350 at 67.673 kb on - strand, within MMJJ_RS00350 at 67.703 kb on + strand, within MMJJ_RS00350 at 67.703 kb on + strand, within MMJJ_RS00350 at 67.768 kb on - strand at 67.771 kb on - strand at 67.801 kb on - strand at 67.801 kb on - strand at 67.801 kb on - strand at 67.802 kb on + strand at 67.803 kb on - strand at 67.803 kb on - strand at 67.803 kb on - strand at 67.804 kb on + strand at 67.804 kb on + strand at 67.804 kb on + strand at 67.804 kb on + strand at 67.804 kb on + strand at 67.808 kb on - strand at 67.808 kb on - strand at 67.808 kb on - strand at 67.809 kb on + strand at 67.827 kb on - strand at 67.827 kb on - strand at 67.829 kb on - strand at 67.829 kb on - strand at 67.829 kb on - strand at 67.829 kb on - strand at 67.830 kb on + strand at 67.830 kb on + strand at 67.830 kb on + strand at 67.830 kb on + strand at 67.830 kb on + strand at 67.830 kb on + strand at 67.831 kb on - strand at 67.831 kb on - strand at 67.831 kb on - strand at 67.831 kb on - strand at 67.832 kb on + strand at 67.832 kb on + strand at 67.832 kb on + strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.834 kb on - strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.835 kb on + strand at 67.836 kb on - strand at 67.836 kb on - strand at 67.837 kb on + strand at 67.837 kb on + strand at 67.837 kb on + strand at 67.837 kb on + strand at 67.837 kb on + strand at 67.849 kb on - strand at 67.849 kb on - strand at 67.850 kb on + strand at 67.853 kb on - strand at 67.853 kb on - strand at 67.853 kb on - strand at 67.853 kb on - strand at 67.854 kb on + strand at 67.854 kb on + strand at 67.854 kb on + strand at 67.854 kb on + strand at 67.857 kb on - strand at 67.857 kb on - strand at 67.857 kb on - strand at 67.858 kb on + strand at 67.858 kb on + strand at 67.858 kb on + strand at 67.858 kb on + strand at 67.858 kb on + strand at 67.863 kb on - strand at 67.863 kb on - strand at 67.881 kb on - strand at 67.881 kb on - strand at 67.882 kb on + strand at 67.895 kb on - strand at 67.902 kb on + strand at 67.960 kb on - strand, within MMJJ_RS00355 at 68.012 kb on - strand, within MMJJ_RS00355 at 68.014 kb on - strand, within MMJJ_RS00355 at 68.014 kb on - strand, within MMJJ_RS00355 at 68.015 kb on + strand, within MMJJ_RS00355 at 68.108 kb on + strand, within MMJJ_RS00355 at 68.170 kb on - strand, within MMJJ_RS00355 at 68.245 kb on - strand at 68.248 kb on - strand at 68.248 kb on - strand at 68.248 kb on - strand at 68.248 kb on - strand at 68.252 kb on - strand at 68.252 kb on - strand at 68.284 kb on + strand at 68.288 kb on + strand at 68.300 kb on + strand at 68.307 kb on + strand at 68.307 kb on + strand at 68.318 kb on + strand at 68.327 kb on - strand at 68.334 kb on - strand at 68.335 kb on + strand at 68.335 kb on + strand at 68.335 kb on + strand at 68.382 kb on + strand at 68.385 kb on - strand at 68.385 kb on - strand at 68.386 kb on + strand at 68.465 kb on - strand at 68.465 kb on - strand at 68.466 kb on + strand at 68.555 kb on - strand, within MMJJ_RS00360 at 68.618 kb on - strand, within MMJJ_RS00360 at 68.679 kb on - strand, within MMJJ_RS00360 at 68.679 kb on - strand, within MMJJ_RS00360 at 68.983 kb on - strand, within MMJJ_RS00360 at 69.378 kb on - strand at 69.378 kb on - strand, within MMJJ_RS00360 at 69.519 kb on - strand, within MMJJ_RS00360 at 70.074 kb on + strand at 70.149 kb on - strand, within MMJJ_RS00365 at 70.150 kb on + strand, within MMJJ_RS00365 at 70.257 kb on - strand, within MMJJ_RS00365 at 70.395 kb on + strand at 70.411 kb on - strand at 70.445 kb on + strand at 70.463 kb on - strand at 70.463 kb on - strand at 70.467 kb on - strand at 70.468 kb on + strand at 70.472 kb on + strand at 70.476 kb on - strand at 70.481 kb on + strand at 70.481 kb on + strand at 70.483 kb on + strand at 70.483 kb on + strand at 70.625 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 67,397 - MMJJ_RS00350 0.40 +1.0 67,397 - MMJJ_RS00350 0.40 -1.0 67,397 - MMJJ_RS00350 0.40 -0.1 67,397 - MMJJ_RS00350 0.40 -2.2 67,398 + MMJJ_RS00350 0.40 -0.3 67,398 + MMJJ_RS00350 0.40 -0.8 67,403 - MMJJ_RS00350 0.41 +0.8 67,404 + MMJJ_RS00350 0.41 -1.0 67,404 + -1.3 67,404 + MMJJ_RS00350 0.41 -0.3 67,408 - MMJJ_RS00350 0.42 -1.9 67,408 - MMJJ_RS00350 0.42 -1.6 67,409 + MMJJ_RS00350 0.42 -0.5 67,409 + MMJJ_RS00350 0.42 -2.4 67,433 - MMJJ_RS00350 0.46 -0.1 67,437 + MMJJ_RS00350 0.46 -2.9 67,439 - MMJJ_RS00350 0.47 -0.8 67,439 - MMJJ_RS00350 0.47 -3.2 67,439 - MMJJ_RS00350 0.47 -0.6 67,439 - MMJJ_RS00350 0.47 -1.7 67,439 - MMJJ_RS00350 0.47 -3.0 67,440 + MMJJ_RS00350 0.47 -1.3 67,451 + MMJJ_RS00350 0.48 -0.3 67,451 + MMJJ_RS00350 0.48 -1.0 67,451 + MMJJ_RS00350 0.48 -1.2 67,453 - -0.8 67,457 + MMJJ_RS00350 0.49 -1.3 67,474 - MMJJ_RS00350 0.52 +0.2 67,474 - MMJJ_RS00350 0.52 -1.0 67,555 - MMJJ_RS00350 0.64 +0.9 67,556 + MMJJ_RS00350 0.65 +0.1 67,581 - MMJJ_RS00350 0.68 +0.0 67,581 - MMJJ_RS00350 0.68 -2.2 67,581 - MMJJ_RS00350 0.68 +0.0 67,581 - MMJJ_RS00350 0.68 +0.3 67,620 - MMJJ_RS00350 0.74 -1.6 67,670 + MMJJ_RS00350 0.82 -0.3 67,670 + MMJJ_RS00350 0.82 -0.5 67,670 + MMJJ_RS00350 0.82 -0.3 67,671 - MMJJ_RS00350 0.82 +1.1 67,671 - MMJJ_RS00350 0.82 -0.6 67,672 + MMJJ_RS00350 0.82 -0.3 67,673 - MMJJ_RS00350 0.82 +0.5 67,703 + MMJJ_RS00350 0.87 -0.8 67,703 + MMJJ_RS00350 0.87 -2.2 67,768 - -0.9 67,771 - -0.7 67,801 - -0.0 67,801 - +1.4 67,801 - +0.3 67,802 + +0.9 67,803 - -1.6 67,803 - +0.4 67,803 - -0.8 67,804 + -0.7 67,804 + -1.9 67,804 + -1.5 67,804 + +0.1 67,804 + -1.2 67,808 - +0.4 67,808 - +1.8 67,808 - +1.3 67,809 + +0.8 67,827 - +0.7 67,827 - +1.6 67,829 - +0.4 67,829 - -0.1 67,829 - +0.2 67,829 - -0.6 67,830 + -0.2 67,830 + +1.1 67,830 + -0.7 67,830 + -0.9 67,830 + +1.0 67,830 + -0.9 67,831 - +0.6 67,831 - -1.4 67,831 - -1.1 67,831 - -1.3 67,832 + -0.2 67,832 + -0.0 67,832 + +1.0 67,834 - -0.4 67,834 - +0.5 67,834 - -0.7 67,834 - -1.2 67,834 - -0.1 67,834 - +0.6 67,834 - +0.6 67,834 - -0.0 67,834 - -0.3 67,835 + -1.1 67,835 + -0.0 67,835 + -0.7 67,835 + +0.7 67,835 + +0.3 67,835 + -0.2 67,835 + +1.2 67,835 + +0.1 67,835 + +0.6 67,835 + -1.8 67,835 + -0.1 67,835 + +0.4 67,835 + +0.7 67,836 - +0.3 67,836 - -0.4 67,837 + -0.2 67,837 + -1.0 67,837 + -0.6 67,837 + -1.2 67,837 + +0.7 67,849 - +0.2 67,849 - +0.9 67,850 + +0.4 67,853 - +0.9 67,853 - +1.5 67,853 - +0.9 67,853 - +0.4 67,854 + +1.3 67,854 + -0.8 67,854 + +0.8 67,854 + -0.4 67,857 - -2.3 67,857 - +0.8 67,857 - -0.5 67,858 + -1.0 67,858 + +0.5 67,858 + -0.7 67,858 + +0.5 67,858 + +0.7 67,863 - -0.2 67,863 - +0.8 67,881 - +0.8 67,881 - +1.0 67,882 + +1.0 67,895 - -0.5 67,902 + +0.0 67,960 - MMJJ_RS00355 0.18 -3.5 68,012 - MMJJ_RS00355 0.34 -1.3 68,014 - MMJJ_RS00355 0.34 -1.8 68,014 - MMJJ_RS00355 0.34 -0.3 68,015 + MMJJ_RS00355 0.35 -2.8 68,108 + MMJJ_RS00355 0.62 -1.6 68,170 - MMJJ_RS00355 0.81 -2.3 68,245 - -0.6 68,248 - +0.2 68,248 - +0.5 68,248 - +0.7 68,248 - +0.1 68,252 - -0.3 68,252 - -1.3 68,284 + -0.2 68,288 + +1.2 68,300 + +1.0 68,307 + -0.7 68,307 + +0.1 68,318 + +0.6 68,327 - -0.3 68,334 - -0.4 68,335 + +0.8 68,335 + -3.2 68,335 + +2.2 68,382 + +0.2 68,385 - -1.3 68,385 - -0.3 68,386 + -0.1 68,465 - -1.5 68,465 - -1.1 68,466 + +0.7 68,555 - MMJJ_RS00360 0.13 -1.9 68,618 - MMJJ_RS00360 0.18 -1.6 68,679 - MMJJ_RS00360 0.23 -0.3 68,679 - MMJJ_RS00360 0.23 -1.3 68,983 - MMJJ_RS00360 0.47 -1.6 69,378 - +1.2 69,378 - MMJJ_RS00360 0.79 -1.0 69,519 - MMJJ_RS00360 0.90 -1.8 70,074 + -1.1 70,149 - MMJJ_RS00365 0.62 -1.6 70,150 + MMJJ_RS00365 0.62 -1.6 70,257 - MMJJ_RS00365 0.76 -2.1 70,395 + -1.8 70,411 - +0.1 70,445 + -0.9 70,463 - +0.3 70,463 - -1.1 70,467 - -1.3 70,468 + -0.8 70,472 + +0.6 70,476 - +0.8 70,481 + -1.0 70,481 + -2.3 70,483 + +0.5 70,483 + +0.9 70,625 - +0.2
Or see this region's nucleotide sequence