Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00165 and MMJJ_RS00170 are separated by 16 nucleotides MMJJ_RS00170 and MMJJ_RS00175 are separated by 19 nucleotides MMJJ_RS00175 and MMJJ_RS00180 are separated by 1 nucleotides
MMJJ_RS00165: MMJJ_RS00165 - F420-dependent thioredoxin reductase, at 30,618 to 31,520
_RS00165
MMJJ_RS00170: MMJJ_RS00170 - thioredoxin family protein, at 31,537 to 31,884
_RS00170
MMJJ_RS00175: MMJJ_RS00175 - cytochrome c biogenesis CcdA family protein, at 31,904 to 32,557
_RS00175
MMJJ_RS00180: MMJJ_RS00180 - DNA topoisomerase I, at 32,559 to 35,030
_RS00180
Position (kb)
31
32 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 30.549 kb on - strand at 30.554 kb on + strand at 30.554 kb on + strand at 30.554 kb on + strand at 30.561 kb on - strand at 30.566 kb on - strand at 30.566 kb on - strand at 30.566 kb on - strand at 30.571 kb on - strand at 30.571 kb on - strand at 30.572 kb on + strand at 30.572 kb on + strand at 30.574 kb on + strand at 30.592 kb on - strand at 30.622 kb on - strand at 30.623 kb on + strand at 30.623 kb on + strand at 30.624 kb on - strand at 30.636 kb on + strand at 30.638 kb on - strand at 30.869 kb on - strand, within MMJJ_RS00165 at 30.870 kb on + strand, within MMJJ_RS00165 at 30.870 kb on + strand, within MMJJ_RS00165 at 30.870 kb on + strand, within MMJJ_RS00165 at 30.885 kb on - strand, within MMJJ_RS00165 at 30.887 kb on - strand, within MMJJ_RS00165 at 30.912 kb on - strand, within MMJJ_RS00165 at 30.913 kb on + strand, within MMJJ_RS00165 at 30.932 kb on + strand, within MMJJ_RS00165 at 30.947 kb on - strand, within MMJJ_RS00165 at 30.947 kb on - strand, within MMJJ_RS00165 at 30.948 kb on + strand, within MMJJ_RS00165 at 30.948 kb on + strand, within MMJJ_RS00165 at 30.951 kb on - strand, within MMJJ_RS00165 at 30.967 kb on - strand, within MMJJ_RS00165 at 30.968 kb on + strand, within MMJJ_RS00165 at 31.002 kb on - strand, within MMJJ_RS00165 at 31.003 kb on + strand, within MMJJ_RS00165 at 31.003 kb on + strand, within MMJJ_RS00165 at 31.064 kb on - strand, within MMJJ_RS00165 at 31.086 kb on - strand, within MMJJ_RS00165 at 31.086 kb on - strand, within MMJJ_RS00165 at 31.086 kb on - strand, within MMJJ_RS00165 at 31.086 kb on - strand, within MMJJ_RS00165 at 31.088 kb on - strand, within MMJJ_RS00165 at 31.088 kb on - strand, within MMJJ_RS00165 at 31.088 kb on - strand, within MMJJ_RS00165 at 31.089 kb on + strand, within MMJJ_RS00165 at 31.093 kb on - strand, within MMJJ_RS00165 at 31.094 kb on + strand, within MMJJ_RS00165 at 31.100 kb on - strand, within MMJJ_RS00165 at 31.114 kb on - strand at 31.120 kb on - strand, within MMJJ_RS00165 at 31.125 kb on + strand, within MMJJ_RS00165 at 31.142 kb on + strand, within MMJJ_RS00165 at 31.180 kb on - strand, within MMJJ_RS00165 at 31.198 kb on - strand, within MMJJ_RS00165 at 31.198 kb on - strand, within MMJJ_RS00165 at 31.253 kb on - strand, within MMJJ_RS00165 at 31.284 kb on + strand, within MMJJ_RS00165 at 31.300 kb on - strand, within MMJJ_RS00165 at 31.304 kb on - strand, within MMJJ_RS00165 at 31.306 kb on - strand, within MMJJ_RS00165 at 31.404 kb on + strand, within MMJJ_RS00165 at 31.481 kb on - strand at 31.481 kb on - strand at 31.482 kb on + strand at 31.562 kb on - strand at 31.562 kb on - strand at 31.566 kb on - strand at 31.566 kb on - strand at 31.567 kb on + strand at 31.567 kb on + strand at 31.568 kb on - strand at 31.569 kb on + strand at 31.569 kb on + strand at 31.569 kb on + strand at 31.569 kb on + strand at 31.593 kb on - strand, within MMJJ_RS00170 at 31.594 kb on + strand, within MMJJ_RS00170 at 31.594 kb on + strand, within MMJJ_RS00170 at 31.616 kb on - strand, within MMJJ_RS00170 at 31.616 kb on - strand, within MMJJ_RS00170 at 31.620 kb on - strand, within MMJJ_RS00170 at 31.621 kb on + strand, within MMJJ_RS00170 at 31.722 kb on - strand, within MMJJ_RS00170 at 31.722 kb on - strand, within MMJJ_RS00170 at 31.722 kb on - strand, within MMJJ_RS00170 at 31.723 kb on + strand, within MMJJ_RS00170 at 31.723 kb on + strand, within MMJJ_RS00170 at 31.723 kb on + strand, within MMJJ_RS00170 at 31.725 kb on + strand, within MMJJ_RS00170 at 31.734 kb on + strand, within MMJJ_RS00170 at 31.768 kb on - strand, within MMJJ_RS00170 at 31.773 kb on - strand, within MMJJ_RS00170 at 31.850 kb on - strand at 31.853 kb on - strand at 31.890 kb on + strand at 31.962 kb on - strand at 31.971 kb on - strand, within MMJJ_RS00175 at 31.972 kb on + strand, within MMJJ_RS00175 at 31.972 kb on + strand, within MMJJ_RS00175 at 31.984 kb on + strand, within MMJJ_RS00175 at 31.988 kb on - strand, within MMJJ_RS00175 at 31.988 kb on - strand, within MMJJ_RS00175 at 31.989 kb on + strand, within MMJJ_RS00175 at 31.990 kb on - strand, within MMJJ_RS00175 at 31.991 kb on + strand, within MMJJ_RS00175 at 31.991 kb on + strand, within MMJJ_RS00175 at 32.005 kb on - strand, within MMJJ_RS00175 at 32.045 kb on + strand, within MMJJ_RS00175 at 32.129 kb on - strand, within MMJJ_RS00175 at 32.248 kb on - strand, within MMJJ_RS00175 at 32.249 kb on + strand, within MMJJ_RS00175 at 32.256 kb on - strand, within MMJJ_RS00175 at 32.272 kb on + strand, within MMJJ_RS00175 at 32.314 kb on - strand, within MMJJ_RS00175 at 32.314 kb on - strand, within MMJJ_RS00175 at 32.315 kb on + strand, within MMJJ_RS00175 at 32.316 kb on - strand, within MMJJ_RS00175 at 32.316 kb on - strand, within MMJJ_RS00175 at 32.317 kb on + strand, within MMJJ_RS00175 at 32.375 kb on - strand, within MMJJ_RS00175 at 32.402 kb on - strand, within MMJJ_RS00175 at 32.430 kb on - strand, within MMJJ_RS00175 at 32.431 kb on + strand, within MMJJ_RS00175 at 32.466 kb on - strand, within MMJJ_RS00175 at 32.482 kb on + strand, within MMJJ_RS00175 at 32.495 kb on + strand at 32.502 kb on + strand at 32.502 kb on + strand at 32.503 kb on - strand at 32.503 kb on - strand at 32.504 kb on + strand at 32.504 kb on + strand at 32.504 kb on + strand at 32.504 kb on + strand at 32.515 kb on - strand at 32.516 kb on + strand at 32.518 kb on + strand at 32.545 kb on + strand at 32.545 kb on + strand at 32.560 kb on - strand at 32.595 kb on + strand at 32.636 kb on - strand at 32.636 kb on - strand at 32.729 kb on - strand at 32.748 kb on - strand at 32.766 kb on + strand at 32.819 kb on - strand, within MMJJ_RS00180 at 32.820 kb on + strand, within MMJJ_RS00180 at 32.822 kb on + strand, within MMJJ_RS00180
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 30,549 - +0.1 30,554 + -0.9 30,554 + -0.1 30,554 + +1.0 30,561 - -0.9 30,566 - +1.5 30,566 - -0.6 30,566 - +0.5 30,571 - +0.5 30,571 - +0.9 30,572 + -0.2 30,572 + +0.6 30,574 + +0.3 30,592 - -0.0 30,622 - -0.8 30,623 + -0.5 30,623 + -0.1 30,624 - -0.7 30,636 + +0.4 30,638 - +0.8 30,869 - MMJJ_RS00165 0.28 +0.6 30,870 + MMJJ_RS00165 0.28 -0.5 30,870 + MMJJ_RS00165 0.28 +1.2 30,870 + MMJJ_RS00165 0.28 -0.9 30,885 - MMJJ_RS00165 0.30 +1.2 30,887 - MMJJ_RS00165 0.30 -0.4 30,912 - MMJJ_RS00165 0.33 -0.2 30,913 + MMJJ_RS00165 0.33 +1.0 30,932 + MMJJ_RS00165 0.35 +0.2 30,947 - MMJJ_RS00165 0.36 +0.2 30,947 - MMJJ_RS00165 0.36 +1.1 30,948 + MMJJ_RS00165 0.37 +0.3 30,948 + MMJJ_RS00165 0.37 +0.7 30,951 - MMJJ_RS00165 0.37 +0.2 30,967 - MMJJ_RS00165 0.39 -0.3 30,968 + MMJJ_RS00165 0.39 +1.1 31,002 - MMJJ_RS00165 0.43 +0.7 31,003 + MMJJ_RS00165 0.43 -1.3 31,003 + MMJJ_RS00165 0.43 +1.2 31,064 - MMJJ_RS00165 0.49 +0.5 31,086 - MMJJ_RS00165 0.52 -0.5 31,086 - MMJJ_RS00165 0.52 -0.2 31,086 - MMJJ_RS00165 0.52 -1.1 31,086 - MMJJ_RS00165 0.52 -0.2 31,088 - MMJJ_RS00165 0.52 +0.2 31,088 - MMJJ_RS00165 0.52 +0.5 31,088 - MMJJ_RS00165 0.52 -0.1 31,089 + MMJJ_RS00165 0.52 -0.7 31,093 - MMJJ_RS00165 0.53 +0.0 31,094 + MMJJ_RS00165 0.53 -0.7 31,100 - MMJJ_RS00165 0.53 +0.7 31,114 - -1.9 31,120 - MMJJ_RS00165 0.56 +0.7 31,125 + MMJJ_RS00165 0.56 +0.1 31,142 + MMJJ_RS00165 0.58 -0.3 31,180 - MMJJ_RS00165 0.62 -0.1 31,198 - MMJJ_RS00165 0.64 -0.3 31,198 - MMJJ_RS00165 0.64 -0.5 31,253 - MMJJ_RS00165 0.70 -0.3 31,284 + MMJJ_RS00165 0.74 +0.4 31,300 - MMJJ_RS00165 0.76 +0.2 31,304 - MMJJ_RS00165 0.76 +1.1 31,306 - MMJJ_RS00165 0.76 -0.7 31,404 + MMJJ_RS00165 0.87 +0.8 31,481 - -0.1 31,481 - -0.8 31,482 + -0.1 31,562 - +0.4 31,562 - +1.3 31,566 - +0.7 31,566 - +0.7 31,567 + -0.3 31,567 + -0.2 31,568 - +0.2 31,569 + -1.3 31,569 + -0.6 31,569 + +0.5 31,569 + -0.2 31,593 - MMJJ_RS00170 0.16 +0.3 31,594 + MMJJ_RS00170 0.16 +0.6 31,594 + MMJJ_RS00170 0.16 +0.8 31,616 - MMJJ_RS00170 0.23 +0.7 31,616 - MMJJ_RS00170 0.23 +0.2 31,620 - MMJJ_RS00170 0.24 +2.1 31,621 + MMJJ_RS00170 0.24 +0.7 31,722 - MMJJ_RS00170 0.53 -1.5 31,722 - MMJJ_RS00170 0.53 +1.9 31,722 - MMJJ_RS00170 0.53 -1.5 31,723 + MMJJ_RS00170 0.53 -0.9 31,723 + MMJJ_RS00170 0.53 +0.3 31,723 + MMJJ_RS00170 0.53 +0.0 31,725 + MMJJ_RS00170 0.54 +0.5 31,734 + MMJJ_RS00170 0.57 +0.2 31,768 - MMJJ_RS00170 0.66 +0.3 31,773 - MMJJ_RS00170 0.68 +1.0 31,850 - -1.6 31,853 - -2.5 31,890 + +0.8 31,962 - +0.6 31,971 - MMJJ_RS00175 0.10 -0.7 31,972 + MMJJ_RS00175 0.10 +1.1 31,972 + MMJJ_RS00175 0.10 -0.2 31,984 + MMJJ_RS00175 0.12 +0.1 31,988 - MMJJ_RS00175 0.13 -2.2 31,988 - MMJJ_RS00175 0.13 +0.6 31,989 + MMJJ_RS00175 0.13 +1.2 31,990 - MMJJ_RS00175 0.13 +0.9 31,991 + MMJJ_RS00175 0.13 -0.6 31,991 + MMJJ_RS00175 0.13 -1.2 32,005 - MMJJ_RS00175 0.15 +0.1 32,045 + MMJJ_RS00175 0.22 +0.6 32,129 - MMJJ_RS00175 0.34 -1.1 32,248 - MMJJ_RS00175 0.53 -2.1 32,249 + MMJJ_RS00175 0.53 +0.3 32,256 - MMJJ_RS00175 0.54 +0.4 32,272 + MMJJ_RS00175 0.56 -0.9 32,314 - MMJJ_RS00175 0.63 +0.7 32,314 - MMJJ_RS00175 0.63 -1.2 32,315 + MMJJ_RS00175 0.63 +0.3 32,316 - MMJJ_RS00175 0.63 +0.8 32,316 - MMJJ_RS00175 0.63 +0.8 32,317 + MMJJ_RS00175 0.63 -0.6 32,375 - MMJJ_RS00175 0.72 +0.5 32,402 - MMJJ_RS00175 0.76 -0.2 32,430 - MMJJ_RS00175 0.80 +0.5 32,431 + MMJJ_RS00175 0.81 +0.8 32,466 - MMJJ_RS00175 0.86 -0.8 32,482 + MMJJ_RS00175 0.88 +0.8 32,495 + -0.2 32,502 + -1.3 32,502 + +0.5 32,503 - +0.7 32,503 - -0.7 32,504 + +1.1 32,504 + +0.9 32,504 + +0.3 32,504 + +0.9 32,515 - +0.8 32,516 + +1.9 32,518 + +0.9 32,545 + +0.0 32,545 + -0.2 32,560 - -0.4 32,595 + +0.5 32,636 - -0.7 32,636 - +1.1 32,729 - -0.3 32,748 - -1.6 32,766 + -0.2 32,819 - MMJJ_RS00180 0.11 -0.6 32,820 + MMJJ_RS00180 0.11 -0.4 32,822 + MMJJ_RS00180 0.11 +0.2
Or see this region's nucleotide sequence