Experiment: PYE with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00742 and CCNA_00743 are separated by 146 nucleotides CCNA_00743 and CCNA_00744 are separated by 241 nucleotides CCNA_00744 and CCNA_00745 are separated by 33 nucleotides CCNA_00745 and CCNA_03935 overlap by 693 nucleotides CCNA_03935 and CCNA_00746 are separated by 251 nucleotides
CCNA_00742: CCNA_00742 - HAD superfamily sugar phosphatase, at 799,937 to 800,797
_00742
CCNA_00743: CCNA_00743 - NnrU family membrane protein, at 800,944 to 801,510
_00743
CCNA_00744: CCNA_00744 - GIY-YIG endonuclease domain protein, at 801,752 to 802,036
_00744
CCNA_00745: CCNA_00745 - hypothetical protein, at 802,070 to 802,774
_00745
CCNA_03935: CCNA_03935 - hypothetical protein, at 802,082 to 802,519
_03935
CCNA_00746: CCNA_00746 - ADA regulatory protein/O6-methylguanine-DNA, at 802,771 to 803,496
_00746
Position (kb) 801
802
803 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 800.855 kb on + strand at 800.855 kb on + strand at 800.858 kb on - strand at 800.863 kb on - strand at 800.863 kb on - strand at 800.963 kb on + strand at 800.973 kb on + strand at 800.973 kb on + strand at 800.981 kb on - strand at 800.981 kb on - strand at 800.981 kb on - strand at 800.990 kb on - strand at 800.992 kb on + strand at 801.039 kb on + strand, within CCNA_00743 at 801.039 kb on + strand, within CCNA_00743 at 801.091 kb on - strand, within CCNA_00743 at 801.130 kb on + strand, within CCNA_00743 at 801.135 kb on + strand, within CCNA_00743 at 801.135 kb on + strand, within CCNA_00743 at 801.177 kb on - strand, within CCNA_00743 at 801.185 kb on + strand, within CCNA_00743 at 801.199 kb on + strand, within CCNA_00743 at 801.199 kb on + strand, within CCNA_00743 at 801.199 kb on + strand, within CCNA_00743 at 801.207 kb on - strand, within CCNA_00743 at 801.207 kb on - strand, within CCNA_00743 at 801.222 kb on + strand, within CCNA_00743 at 801.231 kb on + strand, within CCNA_00743 at 801.231 kb on + strand, within CCNA_00743 at 801.231 kb on + strand, within CCNA_00743 at 801.231 kb on + strand, within CCNA_00743 at 801.231 kb on + strand, within CCNA_00743 at 801.239 kb on - strand, within CCNA_00743 at 801.239 kb on - strand, within CCNA_00743 at 801.239 kb on - strand, within CCNA_00743 at 801.239 kb on - strand, within CCNA_00743 at 801.239 kb on - strand, within CCNA_00743 at 801.244 kb on - strand, within CCNA_00743 at 801.266 kb on + strand, within CCNA_00743 at 801.274 kb on - strand, within CCNA_00743 at 801.291 kb on + strand, within CCNA_00743 at 801.305 kb on - strand, within CCNA_00743 at 801.320 kb on - strand, within CCNA_00743 at 801.322 kb on - strand, within CCNA_00743 at 801.337 kb on + strand, within CCNA_00743 at 801.342 kb on + strand, within CCNA_00743 at 801.342 kb on + strand, within CCNA_00743 at 801.345 kb on - strand, within CCNA_00743 at 801.345 kb on - strand, within CCNA_00743 at 801.392 kb on + strand, within CCNA_00743 at 801.401 kb on + strand, within CCNA_00743 at 801.402 kb on + strand, within CCNA_00743 at 801.402 kb on + strand, within CCNA_00743 at 801.410 kb on - strand, within CCNA_00743 at 801.418 kb on + strand, within CCNA_00743 at 801.418 kb on + strand, within CCNA_00743 at 801.418 kb on + strand, within CCNA_00743 at 801.435 kb on + strand, within CCNA_00743 at 801.438 kb on - strand, within CCNA_00743 at 801.443 kb on - strand, within CCNA_00743 at 801.469 kb on + strand at 801.483 kb on + strand at 801.506 kb on + strand at 801.556 kb on + strand at 801.564 kb on + strand at 801.564 kb on + strand at 801.572 kb on - strand at 801.572 kb on - strand at 801.596 kb on + strand at 801.596 kb on + strand at 801.596 kb on + strand at 801.604 kb on - strand at 801.604 kb on - strand at 801.604 kb on - strand at 801.623 kb on - strand at 801.628 kb on - strand at 801.628 kb on - strand at 801.628 kb on - strand at 801.643 kb on + strand at 801.666 kb on + strand at 801.669 kb on - strand at 801.674 kb on - strand at 801.764 kb on + strand at 801.764 kb on + strand at 801.777 kb on - strand at 801.797 kb on + strand, within CCNA_00744 at 801.801 kb on + strand, within CCNA_00744 at 801.801 kb on + strand, within CCNA_00744 at 801.809 kb on - strand, within CCNA_00744 at 801.812 kb on + strand, within CCNA_00744 at 801.842 kb on + strand, within CCNA_00744 at 801.842 kb on - strand, within CCNA_00744 at 801.862 kb on - strand, within CCNA_00744 at 801.867 kb on - strand, within CCNA_00744 at 801.892 kb on + strand, within CCNA_00744 at 801.892 kb on + strand, within CCNA_00744 at 801.892 kb on + strand, within CCNA_00744 at 801.939 kb on - strand, within CCNA_00744 at 801.945 kb on + strand, within CCNA_00744 at 801.965 kb on - strand, within CCNA_00744 at 801.989 kb on - strand, within CCNA_00744 at 801.989 kb on - strand, within CCNA_00744 at 802.047 kb on - strand at 802.054 kb on - strand at 802.061 kb on + strand at 802.061 kb on + strand at 802.061 kb on + strand at 802.061 kb on + strand at 802.064 kb on - strand at 802.069 kb on - strand at 802.069 kb on - strand at 802.069 kb on - strand at 802.069 kb on - strand at 802.069 kb on - strand at 802.069 kb on - strand at 802.070 kb on + strand at 802.080 kb on - strand at 802.093 kb on + strand at 802.101 kb on - strand at 802.132 kb on + strand at 802.140 kb on - strand at 802.184 kb on + strand at 802.192 kb on - strand at 802.192 kb on - strand at 802.199 kb on + strand at 802.207 kb on - strand at 802.214 kb on - strand at 802.269 kb on - strand at 802.302 kb on + strand at 802.310 kb on - strand at 802.330 kb on + strand at 802.370 kb on + strand at 802.393 kb on - strand at 802.399 kb on - strand at 802.400 kb on + strand at 802.415 kb on + strand at 802.423 kb on - strand at 802.423 kb on - strand at 802.423 kb on - strand at 802.438 kb on + strand at 802.446 kb on + strand at 802.494 kb on + strand at 802.502 kb on - strand at 802.502 kb on - strand at 802.519 kb on - strand at 802.534 kb on - strand, within CCNA_00745 at 802.571 kb on + strand, within CCNA_00745 at 802.578 kb on + strand, within CCNA_00745 at 802.580 kb on - strand, within CCNA_00745 at 802.586 kb on - strand, within CCNA_00745 at 802.594 kb on + strand, within CCNA_00745 at 802.594 kb on + strand, within CCNA_00745 at 802.602 kb on - strand, within CCNA_00745 at 802.618 kb on - strand, within CCNA_00745 at 802.658 kb on + strand, within CCNA_00745 at 802.680 kb on + strand, within CCNA_00745 at 802.685 kb on + strand, within CCNA_00745 at 802.693 kb on - strand, within CCNA_00745 at 802.705 kb on - strand at 802.705 kb on - strand at 802.705 kb on - strand at 802.739 kb on + strand at 802.739 kb on + strand at 802.745 kb on + strand at 802.745 kb on + strand at 802.856 kb on - strand, within CCNA_00746 at 802.865 kb on + strand, within CCNA_00746 at 802.873 kb on - strand, within CCNA_00746 at 802.884 kb on + strand, within CCNA_00746 at 802.897 kb on + strand, within CCNA_00746 at 802.906 kb on - strand, within CCNA_00746 at 802.919 kb on + strand, within CCNA_00746 at 802.919 kb on + strand at 802.919 kb on + strand, within CCNA_00746 at 802.927 kb on - strand, within CCNA_00746 at 802.927 kb on - strand, within CCNA_00746 at 802.927 kb on - strand, within CCNA_00746 at 802.927 kb on - strand, within CCNA_00746 at 802.932 kb on - strand, within CCNA_00746 at 802.950 kb on + strand, within CCNA_00746 at 802.952 kb on + strand at 802.992 kb on - strand, within CCNA_00746 at 803.003 kb on + strand, within CCNA_00746 at 803.003 kb on + strand, within CCNA_00746 at 803.003 kb on + strand, within CCNA_00746 at 803.003 kb on + strand, within CCNA_00746 at 803.011 kb on - strand, within CCNA_00746 at 803.011 kb on - strand, within CCNA_00746 at 803.011 kb on - strand, within CCNA_00746 at 803.020 kb on + strand, within CCNA_00746 at 803.028 kb on - strand, within CCNA_00746 at 803.028 kb on - strand, within CCNA_00746
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nickel (II) chloride 0.3 mM remove 800,855 + -0.8 800,855 + +1.3 800,858 - -0.9 800,863 - +0.7 800,863 - -2.8 800,963 + -0.4 800,973 + +0.1 800,973 + -0.5 800,981 - +0.6 800,981 - +0.8 800,981 - -0.4 800,990 - +0.3 800,992 + +1.3 801,039 + CCNA_00743 0.17 -0.5 801,039 + CCNA_00743 0.17 -0.0 801,091 - CCNA_00743 0.26 -0.3 801,130 + CCNA_00743 0.33 +0.7 801,135 + CCNA_00743 0.34 -0.6 801,135 + CCNA_00743 0.34 -1.7 801,177 - CCNA_00743 0.41 -0.8 801,185 + CCNA_00743 0.43 +0.8 801,199 + CCNA_00743 0.45 -0.9 801,199 + CCNA_00743 0.45 -0.4 801,199 + CCNA_00743 0.45 +0.2 801,207 - CCNA_00743 0.46 -0.6 801,207 - CCNA_00743 0.46 +0.1 801,222 + CCNA_00743 0.49 +1.0 801,231 + CCNA_00743 0.51 -0.4 801,231 + CCNA_00743 0.51 -1.4 801,231 + CCNA_00743 0.51 -0.4 801,231 + CCNA_00743 0.51 -0.3 801,231 + CCNA_00743 0.51 -0.4 801,239 - CCNA_00743 0.52 -0.5 801,239 - CCNA_00743 0.52 +0.2 801,239 - CCNA_00743 0.52 +0.8 801,239 - CCNA_00743 0.52 +1.4 801,239 - CCNA_00743 0.52 +1.0 801,244 - CCNA_00743 0.53 -0.7 801,266 + CCNA_00743 0.57 -0.7 801,274 - CCNA_00743 0.58 -1.3 801,291 + CCNA_00743 0.61 -0.2 801,305 - CCNA_00743 0.64 +0.3 801,320 - CCNA_00743 0.66 +0.5 801,322 - CCNA_00743 0.67 +2.6 801,337 + CCNA_00743 0.69 +0.3 801,342 + CCNA_00743 0.70 +0.1 801,342 + CCNA_00743 0.70 -1.1 801,345 - CCNA_00743 0.71 +0.1 801,345 - CCNA_00743 0.71 -1.3 801,392 + CCNA_00743 0.79 +0.1 801,401 + CCNA_00743 0.81 +1.9 801,402 + CCNA_00743 0.81 -0.5 801,402 + CCNA_00743 0.81 -0.3 801,410 - CCNA_00743 0.82 +1.6 801,418 + CCNA_00743 0.84 -1.6 801,418 + CCNA_00743 0.84 +0.1 801,418 + CCNA_00743 0.84 +0.5 801,435 + CCNA_00743 0.87 -0.6 801,438 - CCNA_00743 0.87 +2.8 801,443 - CCNA_00743 0.88 +1.3 801,469 + -1.6 801,483 + +0.5 801,506 + -1.0 801,556 + +0.8 801,564 + +0.3 801,564 + -0.6 801,572 - -0.5 801,572 - -1.7 801,596 + +0.0 801,596 + -0.4 801,596 + +1.4 801,604 - +0.9 801,604 - +0.7 801,604 - +0.6 801,623 - -1.1 801,628 - -0.9 801,628 - +0.8 801,628 - +0.0 801,643 + -0.9 801,666 + +1.3 801,669 - +0.4 801,674 - -1.0 801,764 + -1.0 801,764 + -0.8 801,777 - +0.1 801,797 + CCNA_00744 0.16 -1.6 801,801 + CCNA_00744 0.17 -1.2 801,801 + CCNA_00744 0.17 -1.4 801,809 - CCNA_00744 0.20 -0.0 801,812 + CCNA_00744 0.21 -1.3 801,842 + CCNA_00744 0.32 -0.1 801,842 - CCNA_00744 0.32 +0.8 801,862 - CCNA_00744 0.39 -1.4 801,867 - CCNA_00744 0.40 +0.5 801,892 + CCNA_00744 0.49 +0.9 801,892 + CCNA_00744 0.49 -0.7 801,892 + CCNA_00744 0.49 -1.1 801,939 - CCNA_00744 0.66 -1.7 801,945 + CCNA_00744 0.68 -0.4 801,965 - CCNA_00744 0.75 +1.0 801,989 - CCNA_00744 0.83 +0.1 801,989 - CCNA_00744 0.83 -1.2 802,047 - +1.4 802,054 - +1.7 802,061 + +0.1 802,061 + -1.9 802,061 + -0.9 802,061 + -0.4 802,064 - -0.6 802,069 - -0.0 802,069 - -0.1 802,069 - +1.1 802,069 - +1.4 802,069 - +0.2 802,069 - +0.8 802,070 + -1.0 802,080 - +0.3 802,093 + +1.3 802,101 - -0.4 802,132 + -0.8 802,140 - +2.8 802,184 + +0.3 802,192 - -0.4 802,192 - -1.9 802,199 + +0.1 802,207 - +0.2 802,214 - +1.5 802,269 - +0.8 802,302 + +0.8 802,310 - -0.5 802,330 + +1.6 802,370 + -0.9 802,393 - -0.3 802,399 - -0.0 802,400 + +1.6 802,415 + +1.6 802,423 - +0.3 802,423 - -1.0 802,423 - +1.3 802,438 + -0.8 802,446 + +0.7 802,494 + +1.0 802,502 - +1.1 802,502 - -1.6 802,519 - -0.7 802,534 - CCNA_00745 0.66 -0.8 802,571 + CCNA_00745 0.71 -1.2 802,578 + CCNA_00745 0.72 -0.7 802,580 - CCNA_00745 0.72 +1.8 802,586 - CCNA_00745 0.73 +1.9 802,594 + CCNA_00745 0.74 -2.0 802,594 + CCNA_00745 0.74 +1.4 802,602 - CCNA_00745 0.75 -1.2 802,618 - CCNA_00745 0.78 +0.3 802,658 + CCNA_00745 0.83 -0.4 802,680 + CCNA_00745 0.87 -2.3 802,685 + CCNA_00745 0.87 -2.0 802,693 - CCNA_00745 0.88 -1.1 802,705 - -1.1 802,705 - +0.1 802,705 - +0.8 802,739 + -0.8 802,739 + -2.0 802,745 + +0.2 802,745 + +0.6 802,856 - CCNA_00746 0.12 -0.4 802,865 + CCNA_00746 0.13 -0.4 802,873 - CCNA_00746 0.14 -1.3 802,884 + CCNA_00746 0.16 -1.8 802,897 + CCNA_00746 0.17 -0.3 802,906 - CCNA_00746 0.19 -1.5 802,919 + CCNA_00746 0.20 +0.8 802,919 + +2.6 802,919 + CCNA_00746 0.20 +0.4 802,927 - CCNA_00746 0.21 +1.3 802,927 - CCNA_00746 0.21 -1.4 802,927 - CCNA_00746 0.21 +0.1 802,927 - CCNA_00746 0.21 +2.0 802,932 - CCNA_00746 0.22 +0.7 802,950 + CCNA_00746 0.25 +1.0 802,952 + +2.6 802,992 - CCNA_00746 0.30 +1.8 803,003 + CCNA_00746 0.32 +0.9 803,003 + CCNA_00746 0.32 +1.3 803,003 + CCNA_00746 0.32 -0.7 803,003 + CCNA_00746 0.32 +0.4 803,011 - CCNA_00746 0.33 -0.4 803,011 - CCNA_00746 0.33 +0.3 803,011 - CCNA_00746 0.33 +0.2 803,020 + CCNA_00746 0.34 +0.1 803,028 - CCNA_00746 0.35 -0.2 803,028 - CCNA_00746 0.35 -1.6
Or see this region's nucleotide sequence