Experiment: Recovery broth with Ramoplanin 3e-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13265 and BBR_RS20750 are separated by 137 nucleotides BBR_RS20750 and BBR_RS20755 are separated by 1 nucleotides BBR_RS20755 and BBR_RS13270 are separated by 131 nucleotides BBR_RS13270 and BBR_RS13275 are separated by 11 nucleotides
BBR_RS13265: BBR_RS13265 - peptide methionine sulfoxide reductase msrA/msrB, at 750,416 to 750,907
_RS13265
BBR_RS20750: BBR_RS20750 - GntR family transcriptional regulator, at 751,045 to 751,170
_RS20750
BBR_RS20755: BBR_RS20755 - IS3 family transposase, at 751,172 to 751,470
_RS20755
BBR_RS13270: BBR_RS13270 - ABC transporter ATP-binding protein, at 751,602 to 752,414
_RS13270
BBR_RS13275: BBR_RS13275 - hypothetical protein, at 752,426 to 753,721
_RS13275
Position (kb)
751
752
753 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 750.619 kb on - strand, within BBR_RS13265 at 750.652 kb on + strand, within BBR_RS13265 at 750.678 kb on + strand, within BBR_RS13265 at 750.678 kb on + strand, within BBR_RS13265 at 750.705 kb on - strand, within BBR_RS13265 at 750.713 kb on + strand, within BBR_RS13265 at 750.713 kb on + strand, within BBR_RS13265 at 750.713 kb on + strand, within BBR_RS13265 at 750.713 kb on + strand, within BBR_RS13265 at 750.715 kb on + strand, within BBR_RS13265 at 750.716 kb on - strand, within BBR_RS13265 at 750.721 kb on - strand, within BBR_RS13265 at 750.786 kb on + strand, within BBR_RS13265 at 750.794 kb on - strand, within BBR_RS13265 at 750.814 kb on + strand, within BBR_RS13265 at 750.821 kb on + strand, within BBR_RS13265 at 750.837 kb on - strand, within BBR_RS13265 at 750.840 kb on - strand, within BBR_RS13265 at 750.847 kb on - strand, within BBR_RS13265 at 750.847 kb on - strand, within BBR_RS13265 at 750.854 kb on + strand, within BBR_RS13265 at 750.854 kb on + strand, within BBR_RS13265 at 750.902 kb on + strand at 750.902 kb on + strand at 750.910 kb on - strand at 750.910 kb on - strand at 750.921 kb on - strand at 750.931 kb on - strand at 750.973 kb on + strand at 750.981 kb on - strand at 750.981 kb on - strand at 750.981 kb on - strand at 750.982 kb on - strand at 751.088 kb on + strand, within BBR_RS20750 at 751.108 kb on - strand, within BBR_RS20750 at 751.108 kb on - strand, within BBR_RS20750 at 751.160 kb on + strand at 751.160 kb on + strand at 751.162 kb on + strand at 751.162 kb on + strand at 751.162 kb on + strand at 751.162 kb on + strand at 751.170 kb on - strand at 751.212 kb on - strand, within BBR_RS20755 at 751.217 kb on + strand, within BBR_RS20755 at 751.217 kb on + strand, within BBR_RS20755 at 751.225 kb on - strand, within BBR_RS20755 at 751.225 kb on - strand, within BBR_RS20755 at 751.230 kb on + strand, within BBR_RS20755 at 751.230 kb on + strand, within BBR_RS20755 at 751.238 kb on - strand, within BBR_RS20755 at 751.293 kb on - strand, within BBR_RS20755 at 751.345 kb on - strand, within BBR_RS20755 at 751.365 kb on + strand, within BBR_RS20755 at 751.373 kb on - strand, within BBR_RS20755 at 751.413 kb on + strand, within BBR_RS20755 at 751.413 kb on - strand, within BBR_RS20755 at 751.413 kb on - strand, within BBR_RS20755 at 751.490 kb on + strand at 751.490 kb on + strand at 751.490 kb on + strand at 751.498 kb on - strand at 751.498 kb on - strand at 751.498 kb on - strand at 751.498 kb on - strand at 751.498 kb on - strand at 751.498 kb on - strand at 751.519 kb on + strand at 751.596 kb on - strand at 751.597 kb on + strand at 751.624 kb on + strand at 751.671 kb on + strand at 751.679 kb on - strand at 751.689 kb on - strand, within BBR_RS13270 at 751.714 kb on + strand, within BBR_RS13270 at 751.742 kb on - strand, within BBR_RS13270 at 751.744 kb on + strand, within BBR_RS13270 at 751.752 kb on + strand, within BBR_RS13270 at 751.754 kb on + strand, within BBR_RS13270 at 751.754 kb on + strand, within BBR_RS13270 at 751.754 kb on + strand, within BBR_RS13270 at 751.761 kb on + strand, within BBR_RS13270 at 751.768 kb on + strand, within BBR_RS13270 at 751.771 kb on + strand, within BBR_RS13270 at 751.775 kb on + strand, within BBR_RS13270 at 751.833 kb on - strand, within BBR_RS13270 at 751.833 kb on - strand, within BBR_RS13270 at 751.833 kb on - strand, within BBR_RS13270 at 751.843 kb on - strand, within BBR_RS13270 at 751.881 kb on + strand, within BBR_RS13270 at 751.901 kb on - strand, within BBR_RS13270 at 751.951 kb on + strand, within BBR_RS13270 at 751.988 kb on + strand, within BBR_RS13270 at 751.988 kb on + strand, within BBR_RS13270 at 751.988 kb on + strand, within BBR_RS13270 at 752.055 kb on - strand, within BBR_RS13270 at 752.055 kb on - strand, within BBR_RS13270 at 752.097 kb on - strand, within BBR_RS13270 at 752.107 kb on + strand, within BBR_RS13270 at 752.138 kb on + strand, within BBR_RS13270 at 752.162 kb on + strand, within BBR_RS13270 at 752.170 kb on - strand, within BBR_RS13270 at 752.190 kb on - strand, within BBR_RS13270 at 752.209 kb on + strand, within BBR_RS13270 at 752.217 kb on - strand, within BBR_RS13270 at 752.217 kb on - strand, within BBR_RS13270 at 752.271 kb on - strand, within BBR_RS13270 at 752.301 kb on + strand, within BBR_RS13270 at 752.307 kb on - strand, within BBR_RS13270 at 752.361 kb on + strand at 752.366 kb on + strand at 752.366 kb on + strand at 752.366 kb on + strand at 752.366 kb on + strand at 752.388 kb on + strand at 752.389 kb on - strand at 752.408 kb on + strand at 752.408 kb on + strand at 752.468 kb on - strand at 752.492 kb on - strand at 752.517 kb on - strand at 752.556 kb on + strand, within BBR_RS13275 at 752.564 kb on - strand, within BBR_RS13275 at 752.605 kb on - strand, within BBR_RS13275 at 752.626 kb on - strand, within BBR_RS13275 at 752.663 kb on + strand, within BBR_RS13275 at 752.669 kb on + strand, within BBR_RS13275 at 752.783 kb on + strand, within BBR_RS13275 at 752.790 kb on - strand, within BBR_RS13275 at 752.811 kb on - strand, within BBR_RS13275 at 752.813 kb on + strand, within BBR_RS13275 at 752.813 kb on - strand, within BBR_RS13275 at 752.821 kb on - strand, within BBR_RS13275 at 752.844 kb on - strand, within BBR_RS13275 at 752.850 kb on - strand, within BBR_RS13275 at 752.852 kb on + strand, within BBR_RS13275 at 752.912 kb on - strand, within BBR_RS13275 at 752.912 kb on - strand, within BBR_RS13275 at 752.984 kb on + strand, within BBR_RS13275 at 752.999 kb on + strand, within BBR_RS13275 at 753.022 kb on + strand, within BBR_RS13275 at 753.037 kb on + strand, within BBR_RS13275 at 753.045 kb on - strand, within BBR_RS13275 at 753.082 kb on + strand, within BBR_RS13275 at 753.082 kb on + strand, within BBR_RS13275 at 753.097 kb on + strand, within BBR_RS13275 at 753.105 kb on + strand, within BBR_RS13275 at 753.109 kb on + strand, within BBR_RS13275 at 753.157 kb on - strand, within BBR_RS13275 at 753.194 kb on + strand, within BBR_RS13275 at 753.200 kb on - strand, within BBR_RS13275 at 753.204 kb on + strand, within BBR_RS13275 at 753.204 kb on + strand, within BBR_RS13275 at 753.212 kb on - strand, within BBR_RS13275 at 753.216 kb on + strand, within BBR_RS13275 at 753.226 kb on + strand, within BBR_RS13275 at 753.249 kb on - strand, within BBR_RS13275 at 753.292 kb on - strand, within BBR_RS13275 at 753.294 kb on - strand, within BBR_RS13275 at 753.350 kb on - strand, within BBR_RS13275 at 753.375 kb on + strand, within BBR_RS13275
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Ramoplanin 3e-05 mM remove 750,619 - BBR_RS13265 0.41 +0.5 750,652 + BBR_RS13265 0.48 -0.9 750,678 + BBR_RS13265 0.53 -0.0 750,678 + BBR_RS13265 0.53 -0.3 750,705 - BBR_RS13265 0.59 -1.3 750,713 + BBR_RS13265 0.60 -0.7 750,713 + BBR_RS13265 0.60 +0.2 750,713 + BBR_RS13265 0.60 +1.2 750,713 + BBR_RS13265 0.60 -0.5 750,715 + BBR_RS13265 0.61 +0.4 750,716 - BBR_RS13265 0.61 +0.7 750,721 - BBR_RS13265 0.62 -0.1 750,786 + BBR_RS13265 0.75 -0.5 750,794 - BBR_RS13265 0.77 +0.1 750,814 + BBR_RS13265 0.81 +0.4 750,821 + BBR_RS13265 0.82 +0.2 750,837 - BBR_RS13265 0.86 +0.1 750,840 - BBR_RS13265 0.86 +0.6 750,847 - BBR_RS13265 0.88 +0.2 750,847 - BBR_RS13265 0.88 -0.3 750,854 + BBR_RS13265 0.89 -0.6 750,854 + BBR_RS13265 0.89 +0.5 750,902 + -0.1 750,902 + +1.1 750,910 - -0.1 750,910 - -0.4 750,921 - -0.7 750,931 - +0.7 750,973 + -0.9 750,981 - +0.0 750,981 - -0.2 750,981 - -0.9 750,982 - +0.9 751,088 + BBR_RS20750 0.34 +0.5 751,108 - BBR_RS20750 0.50 -0.0 751,108 - BBR_RS20750 0.50 +0.1 751,160 + +1.0 751,160 + -1.2 751,162 + -0.3 751,162 + -0.7 751,162 + +0.8 751,162 + +0.5 751,170 - -0.0 751,212 - BBR_RS20755 0.13 -0.3 751,217 + BBR_RS20755 0.15 +0.8 751,217 + BBR_RS20755 0.15 -0.3 751,225 - BBR_RS20755 0.18 +0.2 751,225 - BBR_RS20755 0.18 +0.7 751,230 + BBR_RS20755 0.19 +0.3 751,230 + BBR_RS20755 0.19 -0.6 751,238 - BBR_RS20755 0.22 -2.2 751,293 - BBR_RS20755 0.40 -0.2 751,345 - BBR_RS20755 0.58 +0.2 751,365 + BBR_RS20755 0.65 +1.1 751,373 - BBR_RS20755 0.67 -0.1 751,413 + BBR_RS20755 0.81 -0.1 751,413 - BBR_RS20755 0.81 +0.3 751,413 - BBR_RS20755 0.81 -0.5 751,490 + -0.4 751,490 + -0.6 751,490 + +0.0 751,498 - -0.3 751,498 - -0.5 751,498 - -1.0 751,498 - -0.0 751,498 - -0.4 751,498 - +0.5 751,519 + +0.2 751,596 - -1.1 751,597 + +0.8 751,624 + -1.4 751,671 + +0.5 751,679 - +0.8 751,689 - BBR_RS13270 0.11 +0.1 751,714 + BBR_RS13270 0.14 +0.8 751,742 - BBR_RS13270 0.17 +0.3 751,744 + BBR_RS13270 0.17 +3.7 751,752 + BBR_RS13270 0.18 +0.8 751,754 + BBR_RS13270 0.19 +0.0 751,754 + BBR_RS13270 0.19 -0.1 751,754 + BBR_RS13270 0.19 -0.1 751,761 + BBR_RS13270 0.20 +1.4 751,768 + BBR_RS13270 0.20 -0.5 751,771 + BBR_RS13270 0.21 -0.3 751,775 + BBR_RS13270 0.21 +1.4 751,833 - BBR_RS13270 0.28 +0.9 751,833 - BBR_RS13270 0.28 -0.9 751,833 - BBR_RS13270 0.28 -1.5 751,843 - BBR_RS13270 0.30 +0.4 751,881 + BBR_RS13270 0.34 +0.7 751,901 - BBR_RS13270 0.37 +0.2 751,951 + BBR_RS13270 0.43 +0.1 751,988 + BBR_RS13270 0.47 +1.6 751,988 + BBR_RS13270 0.47 +0.8 751,988 + BBR_RS13270 0.47 +0.9 752,055 - BBR_RS13270 0.56 +0.6 752,055 - BBR_RS13270 0.56 +1.4 752,097 - BBR_RS13270 0.61 +1.2 752,107 + BBR_RS13270 0.62 +1.1 752,138 + BBR_RS13270 0.66 +0.3 752,162 + BBR_RS13270 0.69 +0.4 752,170 - BBR_RS13270 0.70 +1.2 752,190 - BBR_RS13270 0.72 +1.0 752,209 + BBR_RS13270 0.75 +0.9 752,217 - BBR_RS13270 0.76 -1.2 752,217 - BBR_RS13270 0.76 +1.4 752,271 - BBR_RS13270 0.82 -0.7 752,301 + BBR_RS13270 0.86 +1.0 752,307 - BBR_RS13270 0.87 +0.3 752,361 + -0.9 752,366 + +0.4 752,366 + -1.0 752,366 + +1.1 752,366 + +0.6 752,388 + +1.3 752,389 - -0.1 752,408 + -0.5 752,408 + +0.2 752,468 - +0.5 752,492 - -1.0 752,517 - +0.1 752,556 + BBR_RS13275 0.10 -1.0 752,564 - BBR_RS13275 0.11 +1.0 752,605 - BBR_RS13275 0.14 -0.7 752,626 - BBR_RS13275 0.15 -0.1 752,663 + BBR_RS13275 0.18 +0.3 752,669 + BBR_RS13275 0.19 +0.5 752,783 + BBR_RS13275 0.28 -0.4 752,790 - BBR_RS13275 0.28 +0.7 752,811 - BBR_RS13275 0.30 -0.2 752,813 + BBR_RS13275 0.30 +0.2 752,813 - BBR_RS13275 0.30 -0.9 752,821 - BBR_RS13275 0.30 +1.2 752,844 - BBR_RS13275 0.32 +1.3 752,850 - BBR_RS13275 0.33 +0.7 752,852 + BBR_RS13275 0.33 -0.6 752,912 - BBR_RS13275 0.38 +0.2 752,912 - BBR_RS13275 0.38 +0.3 752,984 + BBR_RS13275 0.43 +0.1 752,999 + BBR_RS13275 0.44 -0.4 753,022 + BBR_RS13275 0.46 +0.7 753,037 + BBR_RS13275 0.47 +0.1 753,045 - BBR_RS13275 0.48 +0.1 753,082 + BBR_RS13275 0.51 -0.2 753,082 + BBR_RS13275 0.51 +0.5 753,097 + BBR_RS13275 0.52 +0.4 753,105 + BBR_RS13275 0.52 -0.0 753,109 + BBR_RS13275 0.53 -1.0 753,157 - BBR_RS13275 0.56 -0.3 753,194 + BBR_RS13275 0.59 +0.4 753,200 - BBR_RS13275 0.60 +0.1 753,204 + BBR_RS13275 0.60 +0.2 753,204 + BBR_RS13275 0.60 +0.3 753,212 - BBR_RS13275 0.61 -0.9 753,216 + BBR_RS13275 0.61 +0.1 753,226 + BBR_RS13275 0.62 +0.5 753,249 - BBR_RS13275 0.64 +0.1 753,292 - BBR_RS13275 0.67 -0.9 753,294 - BBR_RS13275 0.67 +0.2 753,350 - BBR_RS13275 0.71 +0.4 753,375 + BBR_RS13275 0.73 +0.1
Or see this region's nucleotide sequence