Experiment: sodium fluoride 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_2679 and Ac3H11_2680 are separated by 125 nucleotides Ac3H11_2680 and Ac3H11_2681 are separated by 53 nucleotides Ac3H11_2681 and Ac3H11_2682 are separated by 135 nucleotides
Ac3H11_2679 - hypothetical protein, at 974,166 to 974,468
_2679
Ac3H11_2680 - Type I secretion system, outer membrane component LapE, at 974,594 to 975,610
_2680
Ac3H11_2681 - UspA, at 975,664 to 976,110
_2681
Ac3H11_2682 - Polyhydroxyalkanoic acid synthase, at 976,246 to 977,997
_2682
Position (kb) 974
975
976 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 973.892 kb on + strand at 973.893 kb on - strand at 973.893 kb on - strand at 973.893 kb on - strand at 974.035 kb on - strand at 974.058 kb on + strand at 974.059 kb on - strand at 974.059 kb on - strand at 974.059 kb on - strand at 974.232 kb on + strand, within Ac3H11_2679 at 974.232 kb on + strand, within Ac3H11_2679 at 974.232 kb on + strand, within Ac3H11_2679 at 974.232 kb on + strand, within Ac3H11_2679 at 974.232 kb on + strand, within Ac3H11_2679 at 974.233 kb on - strand, within Ac3H11_2679 at 974.233 kb on - strand, within Ac3H11_2679 at 974.233 kb on - strand, within Ac3H11_2679 at 974.233 kb on - strand, within Ac3H11_2679 at 974.331 kb on + strand, within Ac3H11_2679 at 974.331 kb on + strand, within Ac3H11_2679 at 974.331 kb on + strand, within Ac3H11_2679 at 974.331 kb on + strand, within Ac3H11_2679 at 974.332 kb on - strand, within Ac3H11_2679 at 974.484 kb on - strand at 974.517 kb on + strand at 974.518 kb on - strand at 974.518 kb on - strand at 974.518 kb on - strand at 974.609 kb on + strand at 974.609 kb on + strand at 974.609 kb on + strand at 974.609 kb on + strand at 974.610 kb on - strand at 974.678 kb on + strand at 974.746 kb on + strand, within Ac3H11_2680 at 974.755 kb on + strand, within Ac3H11_2680 at 974.755 kb on + strand at 974.773 kb on + strand, within Ac3H11_2680 at 974.773 kb on + strand, within Ac3H11_2680 at 974.774 kb on - strand, within Ac3H11_2680 at 974.816 kb on + strand, within Ac3H11_2680 at 974.894 kb on + strand, within Ac3H11_2680 at 974.906 kb on - strand, within Ac3H11_2680 at 974.909 kb on + strand, within Ac3H11_2680 at 974.910 kb on - strand, within Ac3H11_2680 at 974.910 kb on - strand, within Ac3H11_2680 at 974.910 kb on - strand, within Ac3H11_2680 at 974.910 kb on - strand, within Ac3H11_2680 at 974.910 kb on - strand, within Ac3H11_2680 at 974.935 kb on - strand, within Ac3H11_2680 at 974.961 kb on + strand, within Ac3H11_2680 at 974.962 kb on - strand, within Ac3H11_2680 at 975.212 kb on - strand, within Ac3H11_2680 at 975.231 kb on + strand, within Ac3H11_2680 at 975.232 kb on - strand, within Ac3H11_2680 at 975.232 kb on - strand, within Ac3H11_2680 at 975.232 kb on - strand, within Ac3H11_2680 at 975.312 kb on + strand, within Ac3H11_2680 at 975.312 kb on + strand, within Ac3H11_2680 at 975.312 kb on + strand, within Ac3H11_2680 at 975.312 kb on + strand, within Ac3H11_2680 at 975.312 kb on + strand, within Ac3H11_2680 at 975.312 kb on + strand, within Ac3H11_2680 at 975.312 kb on + strand, within Ac3H11_2680 at 975.312 kb on + strand, within Ac3H11_2680 at 975.312 kb on + strand, within Ac3H11_2680 at 975.313 kb on - strand, within Ac3H11_2680 at 975.313 kb on - strand, within Ac3H11_2680 at 975.313 kb on - strand, within Ac3H11_2680 at 975.313 kb on - strand, within Ac3H11_2680 at 975.313 kb on - strand, within Ac3H11_2680 at 975.313 kb on - strand, within Ac3H11_2680 at 975.313 kb on - strand, within Ac3H11_2680 at 975.313 kb on - strand, within Ac3H11_2680 at 975.313 kb on - strand, within Ac3H11_2680 at 975.430 kb on - strand, within Ac3H11_2680 at 975.477 kb on + strand, within Ac3H11_2680 at 975.840 kb on + strand, within Ac3H11_2681 at 975.840 kb on + strand, within Ac3H11_2681 at 975.840 kb on + strand, within Ac3H11_2681 at 975.841 kb on - strand, within Ac3H11_2681 at 975.841 kb on - strand, within Ac3H11_2681 at 975.886 kb on - strand, within Ac3H11_2681 at 975.997 kb on - strand, within Ac3H11_2681 at 975.998 kb on + strand, within Ac3H11_2681 at 975.998 kb on + strand, within Ac3H11_2681 at 975.998 kb on + strand, within Ac3H11_2681 at 975.998 kb on + strand, within Ac3H11_2681 at 975.998 kb on + strand, within Ac3H11_2681 at 975.998 kb on + strand, within Ac3H11_2681 at 975.998 kb on + strand, within Ac3H11_2681 at 975.998 kb on + strand, within Ac3H11_2681 at 975.998 kb on + strand, within Ac3H11_2681 at 975.998 kb on + strand, within Ac3H11_2681 at 975.998 kb on + strand, within Ac3H11_2681 at 975.998 kb on - strand, within Ac3H11_2681 at 975.999 kb on - strand, within Ac3H11_2681 at 975.999 kb on - strand, within Ac3H11_2681 at 975.999 kb on - strand, within Ac3H11_2681 at 975.999 kb on - strand, within Ac3H11_2681 at 975.999 kb on - strand, within Ac3H11_2681 at 975.999 kb on - strand, within Ac3H11_2681 at 975.999 kb on - strand, within Ac3H11_2681 at 976.119 kb on + strand at 976.120 kb on - strand at 976.154 kb on + strand at 976.155 kb on - strand at 976.172 kb on + strand at 976.173 kb on - strand at 976.180 kb on + strand at 976.201 kb on + strand at 976.201 kb on + strand at 976.202 kb on - strand at 976.202 kb on - strand at 976.208 kb on + strand at 976.208 kb on + strand at 976.224 kb on - strand at 976.235 kb on + strand at 976.255 kb on + strand at 976.255 kb on + strand at 976.255 kb on + strand at 976.255 kb on + strand at 976.255 kb on + strand at 976.256 kb on - strand at 976.256 kb on - strand at 976.256 kb on - strand at 976.256 kb on - strand at 976.256 kb on - strand at 976.256 kb on - strand at 976.256 kb on - strand at 976.259 kb on + strand at 976.259 kb on + strand at 976.259 kb on + strand at 976.259 kb on + strand at 976.259 kb on + strand at 976.260 kb on - strand at 976.290 kb on - strand at 976.309 kb on + strand at 976.309 kb on + strand at 976.309 kb on + strand at 976.309 kb on + strand at 976.309 kb on + strand at 976.309 kb on + strand at 976.310 kb on - strand at 976.310 kb on - strand at 976.424 kb on + strand, within Ac3H11_2682 at 976.424 kb on + strand, within Ac3H11_2682 at 976.424 kb on + strand, within Ac3H11_2682 at 976.424 kb on + strand, within Ac3H11_2682 at 976.425 kb on - strand, within Ac3H11_2682 at 976.425 kb on - strand, within Ac3H11_2682 at 976.425 kb on - strand, within Ac3H11_2682 at 976.425 kb on - strand, within Ac3H11_2682 at 976.425 kb on - strand, within Ac3H11_2682 at 976.425 kb on - strand, within Ac3H11_2682 at 976.425 kb on - strand, within Ac3H11_2682 at 976.425 kb on - strand, within Ac3H11_2682 at 976.425 kb on - strand, within Ac3H11_2682 at 976.425 kb on - strand, within Ac3H11_2682 at 976.425 kb on - strand, within Ac3H11_2682 at 976.457 kb on + strand, within Ac3H11_2682 at 976.457 kb on + strand, within Ac3H11_2682 at 976.457 kb on + strand, within Ac3H11_2682 at 976.467 kb on + strand, within Ac3H11_2682 at 976.559 kb on + strand, within Ac3H11_2682 at 976.559 kb on + strand, within Ac3H11_2682 at 976.559 kb on + strand, within Ac3H11_2682 at 976.559 kb on + strand, within Ac3H11_2682 at 976.581 kb on + strand, within Ac3H11_2682 at 976.581 kb on + strand, within Ac3H11_2682 at 976.582 kb on - strand, within Ac3H11_2682
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 50 mM remove 973,892 + -0.9 973,893 - +0.1 973,893 - -0.5 973,893 - -0.2 974,035 - +0.1 974,058 + +0.1 974,059 - -1.1 974,059 - +0.1 974,059 - +0.6 974,232 + Ac3H11_2679 0.22 +0.5 974,232 + Ac3H11_2679 0.22 -0.1 974,232 + Ac3H11_2679 0.22 +0.8 974,232 + Ac3H11_2679 0.22 -0.7 974,232 + Ac3H11_2679 0.22 -1.2 974,233 - Ac3H11_2679 0.22 +2.1 974,233 - Ac3H11_2679 0.22 +0.5 974,233 - Ac3H11_2679 0.22 +1.7 974,233 - Ac3H11_2679 0.22 -1.3 974,331 + Ac3H11_2679 0.54 -0.3 974,331 + Ac3H11_2679 0.54 +1.7 974,331 + Ac3H11_2679 0.54 +0.3 974,331 + Ac3H11_2679 0.54 +1.0 974,332 - Ac3H11_2679 0.55 -0.9 974,484 - +0.3 974,517 + +0.6 974,518 - -0.3 974,518 - +0.3 974,518 - -0.4 974,609 + +0.7 974,609 + -1.3 974,609 + +0.7 974,609 + -0.2 974,610 - -0.6 974,678 + -0.4 974,746 + Ac3H11_2680 0.15 -0.3 974,755 + Ac3H11_2680 0.16 +0.5 974,755 + +0.7 974,773 + Ac3H11_2680 0.18 +0.6 974,773 + Ac3H11_2680 0.18 -0.2 974,774 - Ac3H11_2680 0.18 -1.3 974,816 + Ac3H11_2680 0.22 +0.2 974,894 + Ac3H11_2680 0.29 +1.1 974,906 - Ac3H11_2680 0.31 -0.1 974,909 + Ac3H11_2680 0.31 -1.0 974,910 - Ac3H11_2680 0.31 +0.4 974,910 - Ac3H11_2680 0.31 -0.9 974,910 - Ac3H11_2680 0.31 +0.6 974,910 - Ac3H11_2680 0.31 +0.7 974,910 - Ac3H11_2680 0.31 +0.3 974,935 - Ac3H11_2680 0.34 -0.1 974,961 + Ac3H11_2680 0.36 -0.0 974,962 - Ac3H11_2680 0.36 +0.2 975,212 - Ac3H11_2680 0.61 +0.2 975,231 + Ac3H11_2680 0.63 +0.4 975,232 - Ac3H11_2680 0.63 -0.5 975,232 - Ac3H11_2680 0.63 +1.1 975,232 - Ac3H11_2680 0.63 -0.4 975,312 + Ac3H11_2680 0.71 -0.0 975,312 + Ac3H11_2680 0.71 +0.3 975,312 + Ac3H11_2680 0.71 -1.5 975,312 + Ac3H11_2680 0.71 -0.4 975,312 + Ac3H11_2680 0.71 -0.1 975,312 + Ac3H11_2680 0.71 +0.0 975,312 + Ac3H11_2680 0.71 -0.4 975,312 + Ac3H11_2680 0.71 +0.3 975,312 + Ac3H11_2680 0.71 +0.7 975,313 - Ac3H11_2680 0.71 -0.1 975,313 - Ac3H11_2680 0.71 +0.2 975,313 - Ac3H11_2680 0.71 -0.1 975,313 - Ac3H11_2680 0.71 +1.4 975,313 - Ac3H11_2680 0.71 +0.1 975,313 - Ac3H11_2680 0.71 +0.6 975,313 - Ac3H11_2680 0.71 +0.6 975,313 - Ac3H11_2680 0.71 +0.5 975,313 - Ac3H11_2680 0.71 +0.6 975,430 - Ac3H11_2680 0.82 -0.7 975,477 + Ac3H11_2680 0.87 +0.3 975,840 + Ac3H11_2681 0.39 +0.5 975,840 + Ac3H11_2681 0.39 -0.0 975,840 + Ac3H11_2681 0.39 -1.1 975,841 - Ac3H11_2681 0.40 -0.3 975,841 - Ac3H11_2681 0.40 -0.2 975,886 - Ac3H11_2681 0.50 +0.4 975,997 - Ac3H11_2681 0.74 +0.8 975,998 + Ac3H11_2681 0.75 +0.5 975,998 + Ac3H11_2681 0.75 -0.9 975,998 + Ac3H11_2681 0.75 +0.1 975,998 + Ac3H11_2681 0.75 -0.3 975,998 + Ac3H11_2681 0.75 -1.4 975,998 + Ac3H11_2681 0.75 +0.9 975,998 + Ac3H11_2681 0.75 -0.6 975,998 + Ac3H11_2681 0.75 +1.0 975,998 + Ac3H11_2681 0.75 +0.4 975,998 + Ac3H11_2681 0.75 -0.1 975,998 + Ac3H11_2681 0.75 -0.3 975,998 - Ac3H11_2681 0.75 +0.2 975,999 - Ac3H11_2681 0.75 +0.5 975,999 - Ac3H11_2681 0.75 +0.2 975,999 - Ac3H11_2681 0.75 -0.4 975,999 - Ac3H11_2681 0.75 -0.1 975,999 - Ac3H11_2681 0.75 -0.2 975,999 - Ac3H11_2681 0.75 -0.7 975,999 - Ac3H11_2681 0.75 -0.1 976,119 + +2.8 976,120 - -0.7 976,154 + -0.4 976,155 - +1.0 976,172 + -1.7 976,173 - +0.6 976,180 + +0.4 976,201 + +0.9 976,201 + +0.1 976,202 - -0.2 976,202 - -1.4 976,208 + +0.3 976,208 + +0.2 976,224 - -0.5 976,235 + +0.5 976,255 + +1.0 976,255 + +0.0 976,255 + -0.3 976,255 + -2.8 976,255 + -0.2 976,256 - -0.0 976,256 - +0.1 976,256 - +0.5 976,256 - +0.1 976,256 - -0.1 976,256 - +0.3 976,256 - +0.6 976,259 + +0.7 976,259 + -0.6 976,259 + -0.2 976,259 + +0.2 976,259 + -0.3 976,260 - +0.4 976,290 - -0.5 976,309 + +1.7 976,309 + +0.3 976,309 + +0.0 976,309 + +0.4 976,309 + +1.3 976,309 + +0.1 976,310 - -0.4 976,310 - -0.5 976,424 + Ac3H11_2682 0.10 +0.7 976,424 + Ac3H11_2682 0.10 +1.3 976,424 + Ac3H11_2682 0.10 +0.5 976,424 + Ac3H11_2682 0.10 +1.1 976,425 - Ac3H11_2682 0.10 -0.4 976,425 - Ac3H11_2682 0.10 +1.4 976,425 - Ac3H11_2682 0.10 +0.9 976,425 - Ac3H11_2682 0.10 -0.0 976,425 - Ac3H11_2682 0.10 -1.3 976,425 - Ac3H11_2682 0.10 -0.7 976,425 - Ac3H11_2682 0.10 -0.8 976,425 - Ac3H11_2682 0.10 -1.4 976,425 - Ac3H11_2682 0.10 -0.1 976,425 - Ac3H11_2682 0.10 +0.7 976,425 - Ac3H11_2682 0.10 +0.7 976,457 + Ac3H11_2682 0.12 +0.1 976,457 + Ac3H11_2682 0.12 +0.1 976,457 + Ac3H11_2682 0.12 -1.0 976,467 + Ac3H11_2682 0.13 -0.1 976,559 + Ac3H11_2682 0.18 -0.8 976,559 + Ac3H11_2682 0.18 +1.1 976,559 + Ac3H11_2682 0.18 -0.5 976,559 + Ac3H11_2682 0.18 +0.0 976,581 + Ac3H11_2682 0.19 +0.1 976,581 + Ac3H11_2682 0.19 -0.7 976,582 - Ac3H11_2682 0.19 -2.5
Or see this region's nucleotide sequence