Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3406 and Shewana3_3407 overlap by 1 nucleotides Shewana3_3407 and panB are separated by 56 nucleotides panB and panC are separated by 76 nucleotides
Shewana3_3406: Shewana3_3406 - poly(A) polymerase (RefSeq), at 4,074,921 to 4,076,228
_3406
Shewana3_3407: Shewana3_3407 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (RefSeq), at 4,076,228 to 4,076,716
_3407
Shewana3_3408: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase (RefSeq), at 4,076,773 to 4,077,567
panB
Shewana3_3409: panC - pantoate--beta-alanine ligase (RefSeq), at 4,077,644 to 4,078,489
panC
Position (kb)
4076
4077
4078 Strain fitness (log2 ratio)
-1
0
1 at 4075.984 kb on + strand, within Shewana3_3406 at 4075.984 kb on + strand, within Shewana3_3406 at 4076.019 kb on + strand, within Shewana3_3406 at 4076.048 kb on + strand, within Shewana3_3406 at 4076.073 kb on + strand, within Shewana3_3406 at 4076.890 kb on + strand, within panB at 4077.047 kb on + strand, within panB at 4077.081 kb on + strand, within panB at 4077.163 kb on + strand, within panB at 4077.433 kb on + strand, within panB at 4077.443 kb on + strand, within panB at 4078.547 kb on - strand at 4078.547 kb on - strand at 4078.547 kb on - strand at 4078.547 kb on - strand at 4078.552 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 4,075,984 + Shewana3_3406 0.81 +0.8 4,075,984 + Shewana3_3406 0.81 -1.0 4,076,019 + Shewana3_3406 0.84 +0.9 4,076,048 + Shewana3_3406 0.86 +0.5 4,076,073 + Shewana3_3406 0.88 +0.6 4,076,890 + panB Shewana3_3408 0.15 -1.7 4,077,047 + panB Shewana3_3408 0.34 -0.4 4,077,081 + panB Shewana3_3408 0.39 -1.7 4,077,163 + panB Shewana3_3408 0.49 -1.1 4,077,433 + panB Shewana3_3408 0.83 -0.9 4,077,443 + panB Shewana3_3408 0.84 -1.2 4,078,547 - +0.4 4,078,547 - -0.1 4,078,547 - +0.2 4,078,547 - +0.6 4,078,552 - +0.9
Or see this region's nucleotide sequence