Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3215 and Shewana3_3216 overlap by 4 nucleotides Shewana3_3216 and obgE are separated by 27 nucleotides obgE and rpmA are separated by 212 nucleotides rpmA and rplU are separated by 14 nucleotides
Shewana3_3215: Shewana3_3215 - hypothetical protein (RefSeq), at 3,859,343 to 3,859,816
_3215
Shewana3_3216: Shewana3_3216 - hypothetical protein (RefSeq), at 3,859,813 to 3,860,574
_3216
Shewana3_3217: obgE - GTPase ObgE (RefSeq), at 3,860,602 to 3,861,768
obgE
Shewana3_3218: rpmA - 50S ribosomal protein L27 (RefSeq), at 3,861,981 to 3,862,235
rpmA
Shewana3_3219: rplU - 50S ribosomal protein L21 (RefSeq), at 3,862,250 to 3,862,561
rplU
Position (kb) 3860
3861
3862 Strain fitness (log2 ratio) -1
0
1 at 3859.937 kb on - strand, within Shewana3_3216 at 3860.205 kb on - strand, within Shewana3_3216 at 3860.409 kb on - strand, within Shewana3_3216 at 3860.463 kb on - strand, within Shewana3_3216 at 3860.539 kb on - strand at 3860.698 kb on - strand at 3860.728 kb on - strand, within obgE
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 3,859,937 - Shewana3_3216 0.16 -0.1 3,860,205 - Shewana3_3216 0.51 +0.4 3,860,409 - Shewana3_3216 0.78 +0.5 3,860,463 - Shewana3_3216 0.85 -1.1 3,860,539 - -0.5 3,860,698 - +1.7 3,860,728 - obgE Shewana3_3217 0.11 +0.9
Or see this region's nucleotide sequence