Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3212 and Shewana3_3213 are separated by 365 nucleotides Shewana3_3213 and Shewana3_3214 are separated by 202 nucleotides Shewana3_3214 and Shewana3_3215 are separated by 22 nucleotides
Shewana3_3212: Shewana3_3212 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 3,855,835 to 3,857,856
_3212
Shewana3_3213: Shewana3_3213 - hypothetical protein (RefSeq), at 3,858,222 to 3,858,635
_3213
Shewana3_3214: Shewana3_3214 - dihydrofolate reductase (RefSeq), at 3,858,838 to 3,859,320
_3214
Shewana3_3215: Shewana3_3215 - hypothetical protein (RefSeq), at 3,859,343 to 3,859,816
_3215
Position (kb)
3858
3859 Strain fitness (log2 ratio)
-1
0
1 at 3857.308 kb on + strand, within Shewana3_3212 at 3857.486 kb on - strand, within Shewana3_3212 at 3857.527 kb on + strand, within Shewana3_3212 at 3857.591 kb on - strand, within Shewana3_3212 at 3858.065 kb on + strand at 3858.281 kb on + strand, within Shewana3_3213 at 3858.307 kb on + strand, within Shewana3_3213 at 3858.418 kb on - strand, within Shewana3_3213 at 3858.821 kb on + strand at 3859.467 kb on - strand, within Shewana3_3215 at 3859.570 kb on - strand, within Shewana3_3215
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 3,857,308 + Shewana3_3212 0.73 +1.1 3,857,486 - Shewana3_3212 0.82 -1.3 3,857,527 + Shewana3_3212 0.84 +0.3 3,857,591 - Shewana3_3212 0.87 +0.7 3,858,065 + -0.1 3,858,281 + Shewana3_3213 0.14 -1.1 3,858,307 + Shewana3_3213 0.21 -0.5 3,858,418 - Shewana3_3213 0.47 -0.5 3,858,821 + -0.9 3,859,467 - Shewana3_3215 0.26 -0.9 3,859,570 - Shewana3_3215 0.48 -0.9
Or see this region's nucleotide sequence