Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2948 and Shewana3_2949 are separated by 370 nucleotides Shewana3_2949 and Shewana3_2950 are separated by 75 nucleotides Shewana3_2950 and Shewana3_2951 overlap by 11 nucleotides Shewana3_2951 and smpB are separated by 253 nucleotides
Shewana3_2948: Shewana3_2948 - uroporphyrin-III C/tetrapyrrole methyltransferase (RefSeq), at 3,542,442 to 3,543,233
_2948
Shewana3_2949: Shewana3_2949 - SmpA/OmlA domain-containing protein (RefSeq), at 3,543,604 to 3,544,128
_2949
Shewana3_2950: Shewana3_2950 - hypothetical protein (RefSeq), at 3,544,204 to 3,544,539
_2950
Shewana3_2951: Shewana3_2951 - cyclase/dehydrase (RefSeq), at 3,544,529 to 3,544,966
_2951
Shewana3_2952: smpB - SsrA-binding protein (RefSeq), at 3,545,220 to 3,545,711
smpB
Position (kb)
3544
3545 Strain fitness (log2 ratio)
-1
0
1
2 at 3543.310 kb on - strand at 3543.995 kb on + strand, within Shewana3_2949 at 3544.136 kb on + strand at 3544.202 kb on + strand at 3544.210 kb on - strand at 3544.210 kb on - strand at 3544.220 kb on - strand at 3544.247 kb on + strand, within Shewana3_2950 at 3544.255 kb on - strand, within Shewana3_2950 at 3544.255 kb on - strand, within Shewana3_2950
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 3,543,310 - +0.1 3,543,995 + Shewana3_2949 0.74 +1.3 3,544,136 + +0.5 3,544,202 + -1.0 3,544,210 - +0.3 3,544,210 - +0.7 3,544,220 - +1.8 3,544,247 + Shewana3_2950 0.13 -1.1 3,544,255 - Shewana3_2950 0.15 +0.6 3,544,255 - Shewana3_2950 0.15 +1.1
Or see this region's nucleotide sequence