Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2573 and Shewana3_2574 are separated by 83 nucleotides Shewana3_2574 and Shewana3_2575 overlap by 20 nucleotides Shewana3_2575 and Shewana3_2576 are separated by 73 nucleotides Shewana3_2576 and Shewana3_2577 are separated by 350 nucleotides Shewana3_2577 and Shewana3_2578 are separated by 230 nucleotides
Shewana3_2573: Shewana3_2573 - Cytidine deaminase (EC 3.5.4.5) (from data) , at 3,077,679 to 3,078,569
_2573
Shewana3_2574: Shewana3_2574 - hypothetical protein (RefSeq), at 3,078,653 to 3,078,868
_2574
Shewana3_2575: Shewana3_2575 - glyoxalase/bleomycin resistance protein/dioxygenase (RefSeq), at 3,078,849 to 3,079,196
_2575
Shewana3_2576: Shewana3_2576 - hypothetical protein (RefSeq), at 3,079,270 to 3,079,974
_2576
Shewana3_2577: Shewana3_2577 - glyoxalase family protein (RefSeq), at 3,080,325 to 3,080,705
_2577
Shewana3_2578: Shewana3_2578 - hypothetical protein (RefSeq), at 3,080,936 to 3,084,166
_2578
Position (kb)
3079
3080 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3078.276 kb on + strand, within Shewana3_2573 at 3078.284 kb on - strand, within Shewana3_2573 at 3078.374 kb on + strand, within Shewana3_2573 at 3078.469 kb on + strand, within Shewana3_2573 at 3078.584 kb on + strand at 3078.653 kb on - strand at 3078.851 kb on + strand at 3079.281 kb on - strand at 3079.731 kb on - strand, within Shewana3_2576 at 3079.818 kb on - strand, within Shewana3_2576 at 3079.842 kb on - strand, within Shewana3_2576 at 3079.845 kb on - strand, within Shewana3_2576 at 3079.889 kb on + strand, within Shewana3_2576 at 3079.912 kb on - strand at 3079.964 kb on + strand at 3079.985 kb on + strand at 3080.019 kb on + strand at 3080.138 kb on - strand at 3080.761 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 3,078,276 + Shewana3_2573 0.67 +1.7 3,078,284 - Shewana3_2573 0.68 -0.3 3,078,374 + Shewana3_2573 0.78 +1.1 3,078,469 + Shewana3_2573 0.89 +0.0 3,078,584 + -0.0 3,078,653 - +0.0 3,078,851 + +2.0 3,079,281 - +0.8 3,079,731 - Shewana3_2576 0.65 +1.3 3,079,818 - Shewana3_2576 0.78 +1.0 3,079,842 - Shewana3_2576 0.81 -0.0 3,079,845 - Shewana3_2576 0.82 +1.3 3,079,889 + Shewana3_2576 0.88 -2.2 3,079,912 - +1.0 3,079,964 + +1.7 3,079,985 + -0.3 3,080,019 + -1.7 3,080,138 - +1.4 3,080,761 - -0.3
Or see this region's nucleotide sequence