Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2381 and minC are separated by 123 nucleotides minC and Shewana3_2383 are separated by 27 nucleotides Shewana3_2383 and minE are separated by 2 nucleotides minE and Shewana3_2385 are separated by 162 nucleotides
Shewana3_2381: Shewana3_2381 - hypothetical protein (RefSeq), at 2,861,404 to 2,861,682
_2381
Shewana3_2382: minC - septum formation inhibitor (RefSeq), at 2,861,806 to 2,862,471
minC
Shewana3_2383: Shewana3_2383 - septum site-determining protein MinD (RefSeq), at 2,862,499 to 2,863,308
_2383
Shewana3_2384: minE - cell division topological specificity factor MinE (RefSeq), at 2,863,311 to 2,863,568
minE
Shewana3_2385: Shewana3_2385 - ribonuclease D (RefSeq), at 2,863,731 to 2,864,897
_2385
Position (kb)
2862
2863
2864 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2861.506 kb on - strand, within Shewana3_2381 at 2861.642 kb on + strand, within Shewana3_2381 at 2861.731 kb on + strand at 2861.739 kb on - strand at 2861.808 kb on + strand at 2861.847 kb on - strand at 2862.088 kb on - strand, within minC at 2862.088 kb on - strand, within minC at 2862.399 kb on + strand, within minC at 2862.490 kb on + strand at 2862.525 kb on + strand at 2862.926 kb on + strand, within Shewana3_2383 at 2863.079 kb on + strand, within Shewana3_2383 at 2863.526 kb on + strand, within minE at 2863.669 kb on + strand at 2863.677 kb on - strand at 2864.253 kb on - strand, within Shewana3_2385
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 2,861,506 - Shewana3_2381 0.37 +0.3 2,861,642 + Shewana3_2381 0.85 +0.2 2,861,731 + -0.5 2,861,739 - -0.8 2,861,808 + +0.2 2,861,847 - +1.2 2,862,088 - minC Shewana3_2382 0.42 +2.2 2,862,088 - minC Shewana3_2382 0.42 +2.2 2,862,399 + minC Shewana3_2382 0.89 -0.5 2,862,490 + -0.4 2,862,525 + +0.0 2,862,926 + Shewana3_2383 0.53 -2.0 2,863,079 + Shewana3_2383 0.72 -0.6 2,863,526 + minE Shewana3_2384 0.83 +1.0 2,863,669 + +1.2 2,863,677 - -0.6 2,864,253 - Shewana3_2385 0.45 -0.6
Or see this region's nucleotide sequence