Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2318 and def are separated by 210 nucleotides def and Shewana3_2320 are separated by 192 nucleotides Shewana3_2320 and Shewana3_2321 are separated by 129 nucleotides
Shewana3_2318: Shewana3_2318 - hypothetical protein (RefSeq), at 2,791,094 to 2,791,942
_2318
Shewana3_2319: def - peptide deformylase (RefSeq), at 2,792,153 to 2,792,644
def
Shewana3_2320: Shewana3_2320 - methylated-DNA-(protein)-cysteine S-methyltransferase (RefSeq), at 2,792,837 to 2,793,295
_2320
Shewana3_2321: Shewana3_2321 - hypothetical protein (RefSeq), at 2,793,425 to 2,794,237
_2321
Position (kb)
2792
2793 Strain fitness (log2 ratio)
-1
0
1
2 at 2791.429 kb on - strand, within Shewana3_2318 at 2791.577 kb on + strand, within Shewana3_2318 at 2791.814 kb on - strand, within Shewana3_2318 at 2792.300 kb on - strand, within def at 2792.306 kb on + strand, within def at 2792.903 kb on - strand, within Shewana3_2320 at 2792.913 kb on - strand, within Shewana3_2320 at 2793.055 kb on - strand, within Shewana3_2320 at 2793.072 kb on + strand, within Shewana3_2320 at 2793.215 kb on - strand, within Shewana3_2320 at 2793.327 kb on - strand at 2793.349 kb on - strand at 2793.473 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 2,791,429 - Shewana3_2318 0.39 -1.7 2,791,577 + Shewana3_2318 0.57 -0.4 2,791,814 - Shewana3_2318 0.85 +0.3 2,792,300 - def Shewana3_2319 0.30 +1.2 2,792,306 + def Shewana3_2319 0.31 +1.2 2,792,903 - Shewana3_2320 0.14 -1.6 2,792,913 - Shewana3_2320 0.17 -0.3 2,793,055 - Shewana3_2320 0.47 +0.7 2,793,072 + Shewana3_2320 0.51 +2.2 2,793,215 - Shewana3_2320 0.82 -0.3 2,793,327 - +0.2 2,793,349 - +0.4 2,793,473 - +1.2
Or see this region's nucleotide sequence