Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2114 and Shewana3_2115 are separated by 55 nucleotides Shewana3_2115 and Shewana3_2116 are separated by 142 nucleotides Shewana3_2116 and Shewana3_2117 are separated by 77 nucleotides
Shewana3_2114: Shewana3_2114 - hypothetical protein (RefSeq), at 2,538,054 to 2,538,746
_2114
Shewana3_2115: Shewana3_2115 - fumarate/nitrate reduction transcriptional regulator (RefSeq), at 2,538,802 to 2,539,554
_2115
Shewana3_2116: Shewana3_2116 - universal stress protein UspE (RefSeq), at 2,539,697 to 2,540,629
_2116
Shewana3_2117: Shewana3_2117 - PP-loop domain-containing protein (RefSeq), at 2,540,707 to 2,541,675
_2117
Position (kb)
2539
2540
2541 Strain fitness (log2 ratio)
-1
0
1
2 at 2538.748 kb on - strand at 2539.154 kb on + strand, within Shewana3_2115 at 2539.554 kb on + strand at 2539.666 kb on + strand at 2540.386 kb on - strand, within Shewana3_2116 at 2541.020 kb on + strand, within Shewana3_2117 at 2541.429 kb on + strand, within Shewana3_2117
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 2,538,748 - +2.3 2,539,154 + Shewana3_2115 0.47 -0.5 2,539,554 + +1.0 2,539,666 + +2.3 2,540,386 - Shewana3_2116 0.74 +0.7 2,541,020 + Shewana3_2117 0.32 -1.4 2,541,429 + Shewana3_2117 0.75 +1.4
Or see this region's nucleotide sequence