Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2110 and Shewana3_2111 are separated by 110 nucleotides Shewana3_2111 and Shewana3_2112 are separated by 10 nucleotides Shewana3_2112 and Shewana3_2113 are separated by 19 nucleotides Shewana3_2113 and Shewana3_2114 are separated by 8 nucleotides Shewana3_2114 and Shewana3_2115 are separated by 55 nucleotides
Shewana3_2110: Shewana3_2110 - cytochrome c oxidase, cbb3-type, subunit III (RefSeq), at 2,533,845 to 2,534,813
_2110
Shewana3_2111: Shewana3_2111 - hypothetical protein (RefSeq), at 2,534,924 to 2,535,403
_2111
Shewana3_2112: Shewana3_2112 - heavy metal translocating P-type ATPase (RefSeq), at 2,535,414 to 2,537,825
_2112
Shewana3_2113: Shewana3_2113 - cytochrome oxidase maturation protein, cbb3-type (RefSeq), at 2,537,845 to 2,538,045
_2113
Shewana3_2114: Shewana3_2114 - hypothetical protein (RefSeq), at 2,538,054 to 2,538,746
_2114
Shewana3_2115: Shewana3_2115 - fumarate/nitrate reduction transcriptional regulator (RefSeq), at 2,538,802 to 2,539,554
_2115
Position (kb)
2535
2536
2537
2538 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2534.528 kb on + strand, within Shewana3_2110 at 2534.596 kb on + strand, within Shewana3_2110 at 2535.108 kb on - strand, within Shewana3_2111 at 2535.166 kb on - strand, within Shewana3_2111 at 2535.168 kb on - strand, within Shewana3_2111 at 2535.168 kb on - strand, within Shewana3_2111 at 2535.290 kb on - strand, within Shewana3_2111 at 2535.299 kb on + strand, within Shewana3_2111 at 2535.687 kb on + strand, within Shewana3_2112 at 2535.756 kb on - strand, within Shewana3_2112 at 2536.380 kb on + strand, within Shewana3_2112 at 2536.567 kb on + strand, within Shewana3_2112 at 2536.568 kb on + strand, within Shewana3_2112 at 2536.722 kb on + strand, within Shewana3_2112 at 2536.722 kb on + strand, within Shewana3_2112 at 2536.722 kb on + strand, within Shewana3_2112 at 2536.730 kb on - strand, within Shewana3_2112 at 2536.856 kb on - strand, within Shewana3_2112 at 2537.286 kb on - strand, within Shewana3_2112 at 2537.377 kb on + strand, within Shewana3_2112 at 2537.433 kb on + strand, within Shewana3_2112 at 2537.553 kb on + strand, within Shewana3_2112 at 2537.636 kb on + strand at 2538.031 kb on + strand at 2538.163 kb on + strand, within Shewana3_2114 at 2538.387 kb on + strand, within Shewana3_2114 at 2538.516 kb on - strand, within Shewana3_2114 at 2538.536 kb on - strand, within Shewana3_2114 at 2538.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 2,534,528 + Shewana3_2110 0.70 -0.5 2,534,596 + Shewana3_2110 0.78 -1.1 2,535,108 - Shewana3_2111 0.38 +0.1 2,535,166 - Shewana3_2111 0.50 -1.5 2,535,168 - Shewana3_2111 0.51 -1.3 2,535,168 - Shewana3_2111 0.51 -1.5 2,535,290 - Shewana3_2111 0.76 -0.9 2,535,299 + Shewana3_2111 0.78 +1.4 2,535,687 + Shewana3_2112 0.11 -0.6 2,535,756 - Shewana3_2112 0.14 -1.2 2,536,380 + Shewana3_2112 0.40 -3.1 2,536,567 + Shewana3_2112 0.48 -0.5 2,536,568 + Shewana3_2112 0.48 -1.9 2,536,722 + Shewana3_2112 0.54 -1.5 2,536,722 + Shewana3_2112 0.54 -1.0 2,536,722 + Shewana3_2112 0.54 -0.2 2,536,730 - Shewana3_2112 0.55 -0.8 2,536,856 - Shewana3_2112 0.60 -0.7 2,537,286 - Shewana3_2112 0.78 -2.3 2,537,377 + Shewana3_2112 0.81 -1.5 2,537,433 + Shewana3_2112 0.84 -1.2 2,537,553 + Shewana3_2112 0.89 -0.4 2,537,636 + +0.4 2,538,031 + +1.8 2,538,163 + Shewana3_2114 0.16 +0.4 2,538,387 + Shewana3_2114 0.48 -1.7 2,538,516 - Shewana3_2114 0.67 -1.6 2,538,536 - Shewana3_2114 0.70 +0.9 2,538,748 - +2.3
Or see this region's nucleotide sequence