Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1964 and Shewana3_1965 are separated by 165 nucleotides Shewana3_1965 and Shewana3_1966 are separated by 124 nucleotides Shewana3_1966 and Shewana3_1967 are separated by 187 nucleotides
Shewana3_1964: Shewana3_1964 - signal peptide peptidase SppA, 67K type (RefSeq), at 2,336,874 to 2,338,718
_1964
Shewana3_1965: Shewana3_1965 - 2,4-dienoyl-CoA reductase (RefSeq), at 2,338,884 to 2,340,902
_1965
Shewana3_1966: Shewana3_1966 - hypothetical protein (RefSeq), at 2,341,027 to 2,341,482
_1966
Shewana3_1967: Shewana3_1967 - ferredoxin (RefSeq), at 2,341,670 to 2,342,080
_1967
Position (kb)
2338
2339
2340
2341 Strain fitness (log2 ratio)
-1
0
1
2 at 2338.023 kb on + strand, within Shewana3_1964 at 2338.094 kb on - strand, within Shewana3_1964 at 2338.317 kb on + strand, within Shewana3_1964 at 2338.385 kb on - strand, within Shewana3_1964 at 2339.364 kb on - strand, within Shewana3_1965 at 2340.020 kb on + strand, within Shewana3_1965 at 2340.588 kb on + strand, within Shewana3_1965 at 2340.993 kb on + strand at 2340.998 kb on + strand at 2341.584 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 2,338,023 + Shewana3_1964 0.62 +1.3 2,338,094 - Shewana3_1964 0.66 +0.9 2,338,317 + Shewana3_1964 0.78 +1.3 2,338,385 - Shewana3_1964 0.82 +1.3 2,339,364 - Shewana3_1965 0.24 +2.3 2,340,020 + Shewana3_1965 0.56 +0.0 2,340,588 + Shewana3_1965 0.84 -1.0 2,340,993 + +2.3 2,340,998 + +0.7 2,341,584 - +2.3
Or see this region's nucleotide sequence