Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1938 and Shewana3_1939 overlap by 4 nucleotides Shewana3_1939 and thiG are separated by 1 nucleotides thiG and thiH are separated by 2 nucleotides
Shewana3_1938: Shewana3_1938 - UBA/THIF-type NAD/FAD binding protein (RefSeq), at 2,305,690 to 2,306,706
_1938
Shewana3_1939: Shewana3_1939 - thiamine biosynthesis protein ThiS (RefSeq), at 2,306,703 to 2,306,906
_1939
Shewana3_1940: thiG - thiazole synthase (RefSeq), at 2,306,908 to 2,307,666
thiG
Shewana3_1941: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,307,669 to 2,308,784
thiH
Position (kb)
2306
2307
2308 Strain fitness (log2 ratio)
-1
0
1
2 at 2305.960 kb on - strand, within Shewana3_1938 at 2306.164 kb on - strand, within Shewana3_1938 at 2306.166 kb on + strand, within Shewana3_1938 at 2306.350 kb on - strand, within Shewana3_1938 at 2306.457 kb on - strand, within Shewana3_1938 at 2306.803 kb on + strand, within Shewana3_1939 at 2306.845 kb on + strand, within Shewana3_1939 at 2306.853 kb on - strand, within Shewana3_1939 at 2306.993 kb on + strand, within thiG at 2307.113 kb on + strand, within thiG at 2307.253 kb on + strand, within thiG at 2307.563 kb on + strand, within thiG at 2307.634 kb on + strand at 2307.634 kb on + strand at 2307.634 kb on + strand at 2307.642 kb on - strand at 2307.765 kb on - strand at 2307.927 kb on - strand, within thiH at 2308.149 kb on + strand, within thiH at 2308.335 kb on - strand, within thiH at 2308.389 kb on - strand, within thiH at 2308.459 kb on - strand, within thiH at 2308.473 kb on - strand, within thiH at 2308.512 kb on + strand, within thiH at 2308.596 kb on - strand, within thiH at 2308.616 kb on - strand, within thiH at 2308.616 kb on - strand, within thiH at 2308.616 kb on - strand, within thiH at 2308.625 kb on - strand, within thiH at 2308.645 kb on + strand, within thiH
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 2,305,960 - Shewana3_1938 0.27 +0.9 2,306,164 - Shewana3_1938 0.47 +0.7 2,306,166 + Shewana3_1938 0.47 +0.7 2,306,350 - Shewana3_1938 0.65 -0.7 2,306,457 - Shewana3_1938 0.75 -1.3 2,306,803 + Shewana3_1939 0.49 +1.1 2,306,845 + Shewana3_1939 0.70 -0.7 2,306,853 - Shewana3_1939 0.74 +0.3 2,306,993 + thiG Shewana3_1940 0.11 +0.2 2,307,113 + thiG Shewana3_1940 0.27 +1.0 2,307,253 + thiG Shewana3_1940 0.45 +2.4 2,307,563 + thiG Shewana3_1940 0.86 +1.1 2,307,634 + +0.5 2,307,634 + -0.1 2,307,634 + +0.7 2,307,642 - +1.3 2,307,765 - +0.3 2,307,927 - thiH Shewana3_1941 0.23 +0.4 2,308,149 + thiH Shewana3_1941 0.43 -0.2 2,308,335 - thiH Shewana3_1941 0.60 +0.7 2,308,389 - thiH Shewana3_1941 0.65 -1.2 2,308,459 - thiH Shewana3_1941 0.71 +1.3 2,308,473 - thiH Shewana3_1941 0.72 +2.3 2,308,512 + thiH Shewana3_1941 0.76 -0.2 2,308,596 - thiH Shewana3_1941 0.83 +1.3 2,308,616 - thiH Shewana3_1941 0.85 +0.7 2,308,616 - thiH Shewana3_1941 0.85 +0.7 2,308,616 - thiH Shewana3_1941 0.85 +0.3 2,308,625 - thiH Shewana3_1941 0.86 +1.3 2,308,645 + thiH Shewana3_1941 0.87 -1.6
Or see this region's nucleotide sequence