Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metG and Shewana3_1761 are separated by 160 nucleotides Shewana3_1761 and Shewana3_1762 are separated by 11 nucleotides Shewana3_1762 and dcd are separated by 29 nucleotides
Shewana3_1760: metG - methionyl-tRNA synthetase (RefSeq), at 2,073,887 to 2,075,917
metG
Shewana3_1761: Shewana3_1761 - ATP-binding Mrp/Nbp35 family protein (RefSeq), at 2,076,078 to 2,077,193
_1761
Shewana3_1762: Shewana3_1762 - uridine kinase (RefSeq), at 2,077,205 to 2,077,843
_1762
Shewana3_1763: dcd - deoxycytidine triphosphate deaminase (RefSeq), at 2,077,873 to 2,078,454
dcd
Position (kb)
2076
2077
2078 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2076.174 kb on + strand at 2076.184 kb on + strand at 2076.215 kb on + strand, within Shewana3_1761 at 2076.239 kb on + strand, within Shewana3_1761 at 2076.244 kb on + strand, within Shewana3_1761 at 2076.372 kb on + strand, within Shewana3_1761 at 2076.459 kb on + strand, within Shewana3_1761 at 2076.565 kb on - strand, within Shewana3_1761 at 2076.580 kb on + strand, within Shewana3_1761 at 2076.670 kb on + strand, within Shewana3_1761 at 2076.837 kb on + strand, within Shewana3_1761 at 2077.087 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 2,076,174 + +1.7 2,076,184 + +1.6 2,076,215 + Shewana3_1761 0.12 +0.3 2,076,239 + Shewana3_1761 0.14 +0.4 2,076,244 + Shewana3_1761 0.15 -0.6 2,076,372 + Shewana3_1761 0.26 +1.6 2,076,459 + Shewana3_1761 0.34 +2.4 2,076,565 - Shewana3_1761 0.44 +3.2 2,076,580 + Shewana3_1761 0.45 +0.9 2,076,670 + Shewana3_1761 0.53 -1.1 2,076,837 + Shewana3_1761 0.68 +1.7 2,077,087 + +1.3
Or see this region's nucleotide sequence