Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mnmA and Shewana3_1748 are separated by 221 nucleotides Shewana3_1748 and Shewana3_1749 are separated by 73 nucleotides
Shewana3_1747: mnmA - tRNA-specific 2-thiouridylase MnmA (RefSeq), at 2,060,818 to 2,061,936
mnmA
Shewana3_1748: Shewana3_1748 - NUDIX hydrolase (RefSeq), at 2,062,158 to 2,062,649
_1748
Shewana3_1749: Shewana3_1749 - ribosomal large subunit pseudouridine synthase E (RefSeq), at 2,062,723 to 2,063,532
_1749
Position (kb)
2062
2063 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2061.995 kb on - strand at 2062.212 kb on - strand, within Shewana3_1748 at 2062.240 kb on + strand, within Shewana3_1748 at 2062.348 kb on + strand, within Shewana3_1748 at 2062.348 kb on + strand, within Shewana3_1748 at 2062.496 kb on - strand, within Shewana3_1748 at 2062.598 kb on + strand, within Shewana3_1748 at 2062.678 kb on + strand at 2062.793 kb on + strand at 2062.966 kb on - strand, within Shewana3_1749 at 2063.049 kb on - strand, within Shewana3_1749 at 2063.127 kb on - strand, within Shewana3_1749 at 2063.141 kb on - strand, within Shewana3_1749 at 2063.318 kb on - strand, within Shewana3_1749 at 2063.345 kb on + strand, within Shewana3_1749 at 2063.345 kb on - strand, within Shewana3_1749 at 2063.424 kb on - strand, within Shewana3_1749 at 2063.468 kb on + strand at 2063.546 kb on - strand at 2063.598 kb on + strand at 2063.598 kb on + strand at 2063.598 kb on + strand at 2063.598 kb on + strand at 2063.598 kb on + strand at 2063.606 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 2,061,995 - +0.2 2,062,212 - Shewana3_1748 0.11 +1.6 2,062,240 + Shewana3_1748 0.17 +0.5 2,062,348 + Shewana3_1748 0.39 +1.8 2,062,348 + Shewana3_1748 0.39 +1.0 2,062,496 - Shewana3_1748 0.69 -0.2 2,062,598 + Shewana3_1748 0.89 +0.6 2,062,678 + +0.4 2,062,793 + -1.7 2,062,966 - Shewana3_1749 0.30 +3.2 2,063,049 - Shewana3_1749 0.40 -1.2 2,063,127 - Shewana3_1749 0.50 +1.8 2,063,141 - Shewana3_1749 0.52 -0.5 2,063,318 - Shewana3_1749 0.73 -0.1 2,063,345 + Shewana3_1749 0.77 +0.4 2,063,345 - Shewana3_1749 0.77 -1.0 2,063,424 - Shewana3_1749 0.87 -0.1 2,063,468 + +0.2 2,063,546 - -0.4 2,063,598 + +0.8 2,063,598 + +0.4 2,063,598 + +0.5 2,063,598 + +1.2 2,063,598 + +0.4 2,063,606 - +0.4
Or see this region's nucleotide sequence