Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1742 and Shewana3_1743 are separated by 279 nucleotides Shewana3_1743 and Shewana3_1744 are separated by 96 nucleotides Shewana3_1744 and Shewana3_1745 are separated by 126 nucleotides
Shewana3_1742: Shewana3_1742 - leucine dehydrogenase (RefSeq), at 2,055,662 to 2,056,696
_1742
Shewana3_1743: Shewana3_1743 - hypothetical protein (RefSeq), at 2,056,976 to 2,057,434
_1743
Shewana3_1744: Shewana3_1744 - cupin 4 family protein (RefSeq), at 2,057,531 to 2,058,670
_1744
Shewana3_1745: Shewana3_1745 - adenylosuccinate lyase (RefSeq), at 2,058,797 to 2,060,167
_1745
Position (kb)
2057
2058
2059 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2056.707 kb on + strand at 2057.016 kb on + strand at 2057.511 kb on + strand at 2057.528 kb on + strand at 2057.536 kb on - strand at 2057.822 kb on + strand, within Shewana3_1744 at 2057.934 kb on - strand, within Shewana3_1744 at 2057.987 kb on + strand, within Shewana3_1744 at 2057.996 kb on - strand, within Shewana3_1744 at 2058.094 kb on + strand, within Shewana3_1744 at 2058.104 kb on - strand, within Shewana3_1744 at 2058.246 kb on - strand, within Shewana3_1744 at 2058.485 kb on - strand, within Shewana3_1744 at 2058.559 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 2,056,707 + -2.4 2,057,016 + +0.9 2,057,511 + +1.2 2,057,528 + +2.2 2,057,536 - +0.4 2,057,822 + Shewana3_1744 0.26 +2.2 2,057,934 - Shewana3_1744 0.35 +0.2 2,057,987 + Shewana3_1744 0.40 -0.6 2,057,996 - Shewana3_1744 0.41 +3.0 2,058,094 + Shewana3_1744 0.49 +2.2 2,058,104 - Shewana3_1744 0.50 -0.9 2,058,246 - Shewana3_1744 0.63 +1.1 2,058,485 - Shewana3_1744 0.84 +0.3 2,058,559 + -0.4
Or see this region's nucleotide sequence