Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1552 and Shewana3_1553 are separated by 87 nucleotides Shewana3_1553 and pflA are separated by 15 nucleotides pflA and Shewana3_1555 are separated by 63 nucleotides
Shewana3_1552: Shewana3_1552 - acetate kinase (RefSeq), at 1,808,697 to 1,809,896
_1552
Shewana3_1553: Shewana3_1553 - hypothetical protein (RefSeq), at 1,809,984 to 1,810,481
_1553
Shewana3_1554: pflA - pyruvate formate lyase-activating enzyme 1 (RefSeq), at 1,810,497 to 1,811,237
pflA
Shewana3_1555: Shewana3_1555 - formate acetyltransferase (RefSeq), at 1,811,301 to 1,813,583
_1555
Position (kb)
1810
1811
1812 Strain fitness (log2 ratio)
-1
0
1
2 at 1810.002 kb on - strand at 1810.009 kb on - strand at 1810.011 kb on + strand at 1810.213 kb on - strand, within Shewana3_1553 at 1810.266 kb on + strand, within Shewana3_1553 at 1810.475 kb on + strand at 1810.568 kb on - strand at 1810.797 kb on - strand, within pflA at 1810.865 kb on - strand, within pflA at 1810.965 kb on - strand, within pflA at 1811.141 kb on + strand, within pflA at 1811.156 kb on + strand, within pflA at 1811.318 kb on + strand at 1811.374 kb on + strand at 1811.382 kb on - strand at 1811.660 kb on + strand, within Shewana3_1555 at 1811.712 kb on - strand, within Shewana3_1555 at 1811.779 kb on - strand, within Shewana3_1555 at 1811.944 kb on + strand, within Shewana3_1555 at 1812.029 kb on + strand, within Shewana3_1555 at 1812.106 kb on + strand, within Shewana3_1555
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 1,810,002 - +0.1 1,810,009 - -0.6 1,810,011 + +2.1 1,810,213 - Shewana3_1553 0.46 +0.5 1,810,266 + Shewana3_1553 0.57 +2.1 1,810,475 + -1.5 1,810,568 - +1.6 1,810,797 - pflA Shewana3_1554 0.40 -0.9 1,810,865 - pflA Shewana3_1554 0.50 -0.7 1,810,965 - pflA Shewana3_1554 0.63 -0.7 1,811,141 + pflA Shewana3_1554 0.87 +2.1 1,811,156 + pflA Shewana3_1554 0.89 -1.2 1,811,318 + -0.5 1,811,374 + +0.1 1,811,382 - +1.9 1,811,660 + Shewana3_1555 0.16 -0.9 1,811,712 - Shewana3_1555 0.18 +0.5 1,811,779 - Shewana3_1555 0.21 +1.4 1,811,944 + Shewana3_1555 0.28 +0.5 1,812,029 + Shewana3_1555 0.32 +0.3 1,812,106 + Shewana3_1555 0.35 -0.5
Or see this region's nucleotide sequence