Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flhA and flhF are separated by 11 nucleotides flhF and Shewana3_1358 are separated by 4 nucleotides Shewana3_1358 and fliA overlap by 8 nucleotides
Shewana3_1356: flhA - flagellar biosynthesis protein FlhA (RefSeq), at 1,567,858 to 1,569,957
flhA
Shewana3_1357: flhF - flagellar biosynthesis regulator FlhF (RefSeq), at 1,569,969 to 1,571,345
flhF
Shewana3_1358: Shewana3_1358 - cobyrinic acid a,c-diamide synthase (RefSeq), at 1,571,350 to 1,572,231
_1358
Shewana3_1359: fliA - flagellar biosynthesis sigma factor (RefSeq), at 1,572,224 to 1,572,937
fliA
Position (kb)
1569
1570
1571
1572 Strain fitness (log2 ratio)
-1
0
1
2 at 1569.001 kb on + strand, within flhA at 1569.099 kb on + strand, within flhA at 1569.213 kb on + strand, within flhA at 1569.221 kb on + strand, within flhA at 1569.269 kb on + strand, within flhA at 1569.277 kb on - strand, within flhA at 1569.277 kb on - strand, within flhA at 1569.277 kb on - strand, within flhA at 1569.345 kb on - strand, within flhA at 1570.008 kb on + strand at 1570.180 kb on + strand, within flhF at 1570.239 kb on + strand, within flhF at 1570.329 kb on + strand, within flhF at 1570.332 kb on + strand, within flhF at 1570.349 kb on + strand, within flhF at 1570.690 kb on + strand, within flhF at 1570.767 kb on + strand, within flhF at 1570.878 kb on + strand, within flhF at 1571.047 kb on + strand, within flhF at 1571.052 kb on + strand, within flhF at 1571.110 kb on + strand, within flhF at 1571.587 kb on + strand, within Shewana3_1358 at 1571.602 kb on + strand, within Shewana3_1358 at 1571.812 kb on + strand, within Shewana3_1358 at 1572.075 kb on + strand, within Shewana3_1358 at 1572.239 kb on + strand at 1572.288 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 1,569,001 + flhA Shewana3_1356 0.54 +1.3 1,569,099 + flhA Shewana3_1356 0.59 +0.5 1,569,213 + flhA Shewana3_1356 0.65 +1.2 1,569,221 + flhA Shewana3_1356 0.65 +1.4 1,569,269 + flhA Shewana3_1356 0.67 +0.4 1,569,277 - flhA Shewana3_1356 0.68 +1.8 1,569,277 - flhA Shewana3_1356 0.68 +0.2 1,569,277 - flhA Shewana3_1356 0.68 +0.8 1,569,345 - flhA Shewana3_1356 0.71 -1.5 1,570,008 + +0.7 1,570,180 + flhF Shewana3_1357 0.15 +0.9 1,570,239 + flhF Shewana3_1357 0.20 +0.8 1,570,329 + flhF Shewana3_1357 0.26 +1.5 1,570,332 + flhF Shewana3_1357 0.26 +1.0 1,570,349 + flhF Shewana3_1357 0.28 +0.2 1,570,690 + flhF Shewana3_1357 0.52 +0.8 1,570,767 + flhF Shewana3_1357 0.58 +1.0 1,570,878 + flhF Shewana3_1357 0.66 +1.3 1,571,047 + flhF Shewana3_1357 0.78 +0.5 1,571,052 + flhF Shewana3_1357 0.79 +1.5 1,571,110 + flhF Shewana3_1357 0.83 +0.5 1,571,587 + Shewana3_1358 0.27 +1.2 1,571,602 + Shewana3_1358 0.29 +1.6 1,571,812 + Shewana3_1358 0.52 +1.7 1,572,075 + Shewana3_1358 0.82 +1.1 1,572,239 + +1.4 1,572,288 - +0.8
Or see this region's nucleotide sequence