Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgI and flgJ are separated by 17 nucleotides flgJ and Shewana3_1330 are separated by 40 nucleotides
Shewana3_1328: flgI - flagellar basal body P-ring protein (RefSeq), at 1,539,952 to 1,541,043
flgI
Shewana3_1329: flgJ - flagellar rod assembly protein/muramidase FlgJ (RefSeq), at 1,541,061 to 1,542,230
flgJ
Shewana3_1330: Shewana3_1330 - flagellar hook-associated protein FlgK (RefSeq), at 1,542,271 to 1,544,196
_1330
Position (kb)
1541
1542
1543 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1540.135 kb on - strand, within flgI at 1540.320 kb on + strand, within flgI at 1540.394 kb on + strand, within flgI at 1540.401 kb on - strand, within flgI at 1540.403 kb on + strand, within flgI at 1540.411 kb on - strand, within flgI at 1540.574 kb on + strand, within flgI at 1540.617 kb on - strand, within flgI at 1540.642 kb on + strand, within flgI at 1540.843 kb on + strand, within flgI at 1540.869 kb on + strand, within flgI at 1541.157 kb on - strand at 1541.362 kb on - strand, within flgJ at 1541.373 kb on - strand, within flgJ at 1541.798 kb on + strand, within flgJ at 1541.900 kb on - strand, within flgJ at 1541.972 kb on + strand, within flgJ at 1542.067 kb on - strand, within flgJ at 1542.251 kb on + strand at 1542.259 kb on - strand at 1542.274 kb on - strand at 1542.285 kb on + strand at 1542.307 kb on + strand at 1542.380 kb on + strand at 1542.392 kb on - strand at 1542.444 kb on + strand at 1542.619 kb on + strand, within Shewana3_1330 at 1542.665 kb on + strand, within Shewana3_1330 at 1542.670 kb on + strand, within Shewana3_1330 at 1542.756 kb on + strand, within Shewana3_1330 at 1542.830 kb on + strand, within Shewana3_1330 at 1543.156 kb on + strand, within Shewana3_1330 at 1543.156 kb on + strand, within Shewana3_1330 at 1543.213 kb on + strand, within Shewana3_1330
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 1,540,135 - flgI Shewana3_1328 0.17 +0.8 1,540,320 + flgI Shewana3_1328 0.34 +0.6 1,540,394 + flgI Shewana3_1328 0.40 +0.3 1,540,401 - flgI Shewana3_1328 0.41 -2.1 1,540,403 + flgI Shewana3_1328 0.41 +0.4 1,540,411 - flgI Shewana3_1328 0.42 +0.8 1,540,574 + flgI Shewana3_1328 0.57 +0.6 1,540,617 - flgI Shewana3_1328 0.61 +0.2 1,540,642 + flgI Shewana3_1328 0.63 +0.8 1,540,843 + flgI Shewana3_1328 0.82 +1.5 1,540,869 + flgI Shewana3_1328 0.84 -0.3 1,541,157 - -0.5 1,541,362 - flgJ Shewana3_1329 0.26 -2.2 1,541,373 - flgJ Shewana3_1329 0.27 -0.7 1,541,798 + flgJ Shewana3_1329 0.63 -1.5 1,541,900 - flgJ Shewana3_1329 0.72 -0.2 1,541,972 + flgJ Shewana3_1329 0.78 +1.0 1,542,067 - flgJ Shewana3_1329 0.86 +0.6 1,542,251 + +0.7 1,542,259 - +1.8 1,542,274 - +0.8 1,542,285 + +0.1 1,542,307 + +0.5 1,542,380 + -0.5 1,542,392 - +0.8 1,542,444 + +1.4 1,542,619 + Shewana3_1330 0.18 +0.9 1,542,665 + Shewana3_1330 0.20 -0.7 1,542,670 + Shewana3_1330 0.21 +0.8 1,542,756 + Shewana3_1330 0.25 +0.3 1,542,830 + Shewana3_1330 0.29 -1.2 1,543,156 + Shewana3_1330 0.46 +1.5 1,543,156 + Shewana3_1330 0.46 +0.0 1,543,213 + Shewana3_1330 0.49 +0.9
Or see this region's nucleotide sequence