Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1178 and Shewana3_1179 are separated by 4 nucleotides Shewana3_1179 and Shewana3_1180 are separated by 84 nucleotides Shewana3_1180 and Shewana3_1181 are separated by 9 nucleotides Shewana3_1181 and trmB are separated by 179 nucleotides trmB and Shewana3_1183 are separated by 45 nucleotides
Shewana3_1178: Shewana3_1178 - cytochrome B561 (RefSeq), at 1,387,037 to 1,387,588
_1178
Shewana3_1179: Shewana3_1179 - hypothetical protein (RefSeq), at 1,387,593 to 1,388,168
_1179
Shewana3_1180: Shewana3_1180 - hypothetical protein (RefSeq), at 1,388,253 to 1,388,531
_1180
Shewana3_1181: Shewana3_1181 - A/G-specific DNA-adenine glycosylase (RefSeq), at 1,388,541 to 1,389,659
_1181
Shewana3_1182: trmB - tRNA (guanine-N(7)-)-methyltransferase (RefSeq), at 1,389,839 to 1,390,555
trmB
Shewana3_1183: Shewana3_1183 - hypothetical protein (RefSeq), at 1,390,601 to 1,390,927
_1183
Position (kb)
1388
1389
1390 Strain fitness (log2 ratio)
-2
-1
0
1 at 1387.558 kb on + strand at 1388.174 kb on + strand at 1388.250 kb on + strand at 1388.303 kb on - strand, within Shewana3_1180 at 1388.348 kb on + strand, within Shewana3_1180 at 1388.461 kb on + strand, within Shewana3_1180 at 1388.472 kb on - strand, within Shewana3_1180 at 1388.661 kb on + strand, within Shewana3_1181 at 1388.800 kb on + strand, within Shewana3_1181 at 1388.980 kb on + strand, within Shewana3_1181 at 1389.147 kb on - strand, within Shewana3_1181 at 1389.276 kb on + strand, within Shewana3_1181 at 1389.281 kb on + strand, within Shewana3_1181 at 1389.317 kb on + strand, within Shewana3_1181 at 1389.382 kb on + strand, within Shewana3_1181 at 1389.473 kb on - strand, within Shewana3_1181 at 1389.960 kb on + strand, within trmB at 1390.008 kb on + strand, within trmB
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 1,387,558 + +0.4 1,388,174 + -0.3 1,388,250 + -1.5 1,388,303 - Shewana3_1180 0.18 +1.2 1,388,348 + Shewana3_1180 0.34 -2.0 1,388,461 + Shewana3_1180 0.75 -1.4 1,388,472 - Shewana3_1180 0.78 +0.3 1,388,661 + Shewana3_1181 0.11 -1.5 1,388,800 + Shewana3_1181 0.23 -0.7 1,388,980 + Shewana3_1181 0.39 -0.1 1,389,147 - Shewana3_1181 0.54 +0.3 1,389,276 + Shewana3_1181 0.66 +0.9 1,389,281 + Shewana3_1181 0.66 -1.0 1,389,317 + Shewana3_1181 0.69 -2.4 1,389,382 + Shewana3_1181 0.75 -1.5 1,389,473 - Shewana3_1181 0.83 -0.6 1,389,960 + trmB Shewana3_1182 0.17 +0.7 1,390,008 + trmB Shewana3_1182 0.24 +0.7
Or see this region's nucleotide sequence