Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1043 and deoD are separated by 91 nucleotides deoD and Shewana3_1045 are separated by 167 nucleotides Shewana3_1045 and Shewana3_1046 are separated by 79 nucleotides
Shewana3_1043: Shewana3_1043 - phosphopentomutase (RefSeq), at 1,220,286 to 1,221,500
_1043
Shewana3_1044: deoD - purine nucleoside phosphorylase (RefSeq), at 1,221,592 to 1,222,302
deoD
Shewana3_1045: Shewana3_1045 - membrane protein (RefSeq), at 1,222,470 to 1,223,654
_1045
Shewana3_1046: Shewana3_1046 - phosphoserine phosphatase (RefSeq), at 1,223,734 to 1,224,726
_1046
Position (kb)
1222
1223
1224 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1221.836 kb on + strand, within deoD at 1221.884 kb on + strand, within deoD at 1222.020 kb on + strand, within deoD at 1222.076 kb on - strand at 1222.144 kb on + strand, within deoD at 1222.442 kb on - strand at 1222.569 kb on - strand at 1222.883 kb on - strand, within Shewana3_1045 at 1222.892 kb on - strand, within Shewana3_1045 at 1222.945 kb on + strand, within Shewana3_1045 at 1223.354 kb on + strand, within Shewana3_1045 at 1223.357 kb on - strand, within Shewana3_1045 at 1223.936 kb on - strand, within Shewana3_1046 at 1224.044 kb on + strand, within Shewana3_1046 at 1224.060 kb on - strand, within Shewana3_1046 at 1224.308 kb on - strand, within Shewana3_1046 at 1224.397 kb on - strand, within Shewana3_1046 at 1224.521 kb on - strand, within Shewana3_1046 at 1224.521 kb on - strand, within Shewana3_1046 at 1224.521 kb on - strand, within Shewana3_1046 at 1224.541 kb on + strand, within Shewana3_1046
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 1,221,836 + deoD Shewana3_1044 0.34 +0.8 1,221,884 + deoD Shewana3_1044 0.41 +0.4 1,222,020 + deoD Shewana3_1044 0.60 +1.0 1,222,076 - +1.9 1,222,144 + deoD Shewana3_1044 0.78 +1.2 1,222,442 - -0.3 1,222,569 - +0.3 1,222,883 - Shewana3_1045 0.35 -1.2 1,222,892 - Shewana3_1045 0.36 -1.2 1,222,945 + Shewana3_1045 0.40 -1.7 1,223,354 + Shewana3_1045 0.75 -0.1 1,223,357 - Shewana3_1045 0.75 -1.7 1,223,936 - Shewana3_1046 0.20 -1.7 1,224,044 + Shewana3_1046 0.31 -0.8 1,224,060 - Shewana3_1046 0.33 +0.1 1,224,308 - Shewana3_1046 0.58 -0.2 1,224,397 - Shewana3_1046 0.67 +0.5 1,224,521 - Shewana3_1046 0.79 +1.9 1,224,521 - Shewana3_1046 0.79 -2.0 1,224,521 - Shewana3_1046 0.79 +0.4 1,224,541 + Shewana3_1046 0.81 +0.3
Or see this region's nucleotide sequence