Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt infB and rbfA are separated by 62 nucleotides rbfA and Shewana3_1032 overlap by 1 nucleotides Shewana3_1032 and rpsO are separated by 126 nucleotides rpsO and Shewana3_1034 are separated by 86 nucleotides
Shewana3_1030: infB - translation initiation factor IF-2 (RefSeq), at 1,202,214 to 1,204,883
infB
Shewana3_1031: rbfA - ribosome-binding factor A (RefSeq), at 1,204,946 to 1,205,386
rbfA
Shewana3_1032: Shewana3_1032 - tRNA pseudouridine synthase B (RefSeq), at 1,205,386 to 1,206,339
_1032
Shewana3_1033: rpsO - 30S ribosomal protein S15 (RefSeq), at 1,206,466 to 1,206,735
rpsO
Shewana3_1034: Shewana3_1034 - diguanylate cyclase/phosphodiesterase (RefSeq), at 1,206,822 to 1,208,846
_1034
Position (kb) 1205
1206
1207 Strain fitness (log2 ratio) -2
-1
0
1 at 1205.293 kb on - strand, within rbfA at 1205.358 kb on + strand at 1205.667 kb on + strand, within Shewana3_1032 at 1206.307 kb on + strand at 1206.889 kb on + strand at 1207.138 kb on + strand, within Shewana3_1034 at 1207.192 kb on - strand, within Shewana3_1034
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 1,205,293 - rbfA Shewana3_1031 0.79 -1.9 1,205,358 + +0.3 1,205,667 + Shewana3_1032 0.29 +0.4 1,206,307 + +0.8 1,206,889 + -1.2 1,207,138 + Shewana3_1034 0.16 -0.8 1,207,192 - Shewana3_1034 0.18 -0.0
Or see this region's nucleotide sequence