Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0850 and Shewana3_0851 are separated by 153 nucleotides Shewana3_0851 and Shewana3_0852 are separated by 86 nucleotides Shewana3_0852 and pntB are separated by 154 nucleotides
Shewana3_0850: Shewana3_0850 - hypothetical protein (RefSeq), at 987,834 to 988,661
_0850
Shewana3_0851: Shewana3_0851 - TetR family transcriptional regulator (RefSeq), at 988,815 to 989,294
_0851
Shewana3_0852: Shewana3_0852 - hypothetical protein (RefSeq), at 989,381 to 989,770
_0852
Shewana3_0853: pntB - pyridine nucleotide transhydrogenase (RefSeq), at 989,925 to 991,409
pntB
Position (kb) 989
990 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 988.768 kb on + strand at 988.786 kb on - strand at 988.832 kb on + strand at 988.905 kb on + strand, within Shewana3_0851 at 989.106 kb on + strand, within Shewana3_0851 at 989.159 kb on + strand, within Shewana3_0851 at 989.167 kb on - strand, within Shewana3_0851 at 989.557 kb on + strand, within Shewana3_0852 at 989.576 kb on + strand, within Shewana3_0852 at 989.720 kb on - strand, within Shewana3_0852 at 989.767 kb on + strand at 989.856 kb on + strand at 989.864 kb on - strand at 989.864 kb on - strand at 989.923 kb on - strand at 990.014 kb on - strand at 990.105 kb on + strand, within pntB at 990.240 kb on + strand, within pntB at 990.299 kb on - strand, within pntB at 990.368 kb on - strand, within pntB at 990.541 kb on + strand, within pntB at 990.541 kb on + strand, within pntB at 990.549 kb on - strand, within pntB at 990.616 kb on - strand, within pntB at 990.616 kb on - strand, within pntB
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 988,768 + -1.4 988,786 - -1.9 988,832 + -0.5 988,905 + Shewana3_0851 0.19 +0.4 989,106 + Shewana3_0851 0.61 +0.3 989,159 + Shewana3_0851 0.72 +1.1 989,167 - Shewana3_0851 0.73 +1.9 989,557 + Shewana3_0852 0.45 -0.5 989,576 + Shewana3_0852 0.50 -2.9 989,720 - Shewana3_0852 0.87 -1.4 989,767 + +0.5 989,856 + -0.2 989,864 - -0.4 989,864 - +0.2 989,923 - -0.0 990,014 - +0.7 990,105 + pntB Shewana3_0853 0.12 +0.7 990,240 + pntB Shewana3_0853 0.21 -0.0 990,299 - pntB Shewana3_0853 0.25 +0.4 990,368 - pntB Shewana3_0853 0.30 +0.3 990,541 + pntB Shewana3_0853 0.41 -0.0 990,541 + pntB Shewana3_0853 0.41 -1.0 990,549 - pntB Shewana3_0853 0.42 +0.2 990,616 - pntB Shewana3_0853 0.47 +0.3 990,616 - pntB Shewana3_0853 0.47 +0.1
Or see this region's nucleotide sequence