Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argD and Shewana3_0611 are separated by 127 nucleotides Shewana3_0611 and astD are separated by 9 nucleotides
Shewana3_0610: argD - bifunctional N-succinyldiaminopimelate-aminotransferase/acetylornithine transaminase protein (RefSeq), at 714,876 to 716,093
argD
Shewana3_0611: Shewana3_0611 - Arginine N-succinyltransferase (EC 2.3.1.109) <small>(from data)</small>, at 716,221 to 717,240
_0611
Shewana3_0612: astD - succinylglutamic semialdehyde dehydrogenase (RefSeq), at 717,250 to 718,713
astD
Position (kb) 716
717
718 Strain fitness (log2 ratio) -2
-1
0
1 at 715.563 kb on + strand, within argD at 715.571 kb on - strand, within argD at 715.663 kb on + strand, within argD at 715.791 kb on + strand, within argD at 715.791 kb on - strand, within argD at 715.799 kb on - strand, within argD at 715.799 kb on - strand, within argD at 715.833 kb on + strand, within argD at 715.895 kb on + strand, within argD at 715.927 kb on - strand, within argD at 716.026 kb on - strand at 716.065 kb on + strand at 716.294 kb on + strand at 716.549 kb on + strand, within Shewana3_0611 at 716.549 kb on + strand, within Shewana3_0611 at 716.549 kb on + strand, within Shewana3_0611 at 716.557 kb on - strand, within Shewana3_0611 at 716.882 kb on - strand, within Shewana3_0611 at 716.905 kb on + strand, within Shewana3_0611 at 716.926 kb on + strand, within Shewana3_0611 at 716.959 kb on - strand, within Shewana3_0611 at 716.960 kb on + strand, within Shewana3_0611 at 717.173 kb on - strand at 717.634 kb on + strand, within astD at 717.642 kb on - strand, within astD at 717.669 kb on + strand, within astD at 717.676 kb on - strand, within astD at 717.838 kb on + strand, within astD at 717.838 kb on + strand, within astD at 717.971 kb on - strand, within astD at 718.041 kb on - strand, within astD at 718.074 kb on + strand, within astD at 718.074 kb on + strand, within astD at 718.095 kb on + strand, within astD at 718.143 kb on + strand, within astD at 718.169 kb on - strand, within astD at 718.169 kb on - strand, within astD
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 715,563 + argD Shewana3_0610 0.56 +1.0 715,571 - argD Shewana3_0610 0.57 -0.7 715,663 + argD Shewana3_0610 0.65 +0.1 715,791 + argD Shewana3_0610 0.75 +0.7 715,791 - argD Shewana3_0610 0.75 +0.7 715,799 - argD Shewana3_0610 0.76 -0.5 715,799 - argD Shewana3_0610 0.76 -0.4 715,833 + argD Shewana3_0610 0.79 +0.9 715,895 + argD Shewana3_0610 0.84 +0.4 715,927 - argD Shewana3_0610 0.86 +0.1 716,026 - -0.6 716,065 + +0.9 716,294 + +0.3 716,549 + Shewana3_0611 0.32 +0.4 716,549 + Shewana3_0611 0.32 +0.3 716,549 + Shewana3_0611 0.32 -0.5 716,557 - Shewana3_0611 0.33 -1.4 716,882 - Shewana3_0611 0.65 +0.6 716,905 + Shewana3_0611 0.67 +1.0 716,926 + Shewana3_0611 0.69 +0.5 716,959 - Shewana3_0611 0.72 -0.0 716,960 + Shewana3_0611 0.72 +0.9 717,173 - -0.4 717,634 + astD Shewana3_0612 0.26 +0.3 717,642 - astD Shewana3_0612 0.27 -0.8 717,669 + astD Shewana3_0612 0.29 +0.3 717,676 - astD Shewana3_0612 0.29 -1.6 717,838 + astD Shewana3_0612 0.40 +0.6 717,838 + astD Shewana3_0612 0.40 +0.5 717,971 - astD Shewana3_0612 0.49 -0.1 718,041 - astD Shewana3_0612 0.54 -2.6 718,074 + astD Shewana3_0612 0.56 +0.5 718,074 + astD Shewana3_0612 0.56 -0.2 718,095 + astD Shewana3_0612 0.58 -0.2 718,143 + astD Shewana3_0612 0.61 -0.3 718,169 - astD Shewana3_0612 0.63 +0.2 718,169 - astD Shewana3_0612 0.63 -0.2
Or see this region's nucleotide sequence