Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0586 and Shewana3_0587 are separated by 68 nucleotides Shewana3_0587 and Shewana3_0588 are separated by 14 nucleotides Shewana3_0588 and Shewana3_0589 are separated by 80 nucleotides
Shewana3_0586: Shewana3_0586 - MscS mechanosensitive ion channel (RefSeq), at 685,727 to 688,930
_0586
Shewana3_0587: Shewana3_0587 - hypothetical protein (RefSeq), at 688,999 to 689,883
_0587
Shewana3_0588: Shewana3_0588 - phosphatidylserine decarboxylase (RefSeq), at 689,898 to 690,776
_0588
Shewana3_0589: Shewana3_0589 - ribosome-associated GTPase (RefSeq), at 690,857 to 691,921
_0589
Position (kb) 689
690
691 Strain fitness (log2 ratio) -2
-1
0
1 at 689.200 kb on + strand, within Shewana3_0587 at 689.200 kb on + strand, within Shewana3_0587 at 689.252 kb on - strand, within Shewana3_0587 at 689.431 kb on + strand, within Shewana3_0587 at 689.439 kb on - strand, within Shewana3_0587 at 689.462 kb on - strand, within Shewana3_0587 at 689.462 kb on - strand, within Shewana3_0587 at 689.640 kb on + strand, within Shewana3_0587 at 689.640 kb on + strand, within Shewana3_0587 at 689.658 kb on + strand, within Shewana3_0587 at 689.740 kb on + strand, within Shewana3_0587 at 689.748 kb on - strand, within Shewana3_0587 at 689.784 kb on + strand, within Shewana3_0587 at 689.837 kb on + strand at 689.865 kb on + strand at 690.813 kb on - strand at 690.818 kb on - strand at 690.879 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 689,200 + Shewana3_0587 0.23 -0.6 689,200 + Shewana3_0587 0.23 -0.5 689,252 - Shewana3_0587 0.29 -0.8 689,431 + Shewana3_0587 0.49 -2.3 689,439 - Shewana3_0587 0.50 -1.4 689,462 - Shewana3_0587 0.52 -0.3 689,462 - Shewana3_0587 0.52 -0.9 689,640 + Shewana3_0587 0.72 -0.7 689,640 + Shewana3_0587 0.72 -0.3 689,658 + Shewana3_0587 0.74 -1.0 689,740 + Shewana3_0587 0.84 +0.0 689,748 - Shewana3_0587 0.85 -0.1 689,784 + Shewana3_0587 0.89 -0.4 689,837 + -1.0 689,865 + +1.2 690,813 - -0.5 690,818 - +0.7 690,879 - -0.9
Or see this region's nucleotide sequence