Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0583 and Shewana3_0584 are separated by 90 nucleotides Shewana3_0584 and Shewana3_0585 are separated by 23 nucleotides Shewana3_0585 and Shewana3_0586 are separated by 15 nucleotides
Shewana3_0583: Shewana3_0583 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding (RefSeq), at 683,607 to 684,539
_0583
Shewana3_0584: Shewana3_0584 - hypothetical protein (RefSeq), at 684,630 to 684,965
_0584
Shewana3_0585: Shewana3_0585 - glycerophosphoryl diester phosphodiesterase (RefSeq), at 684,989 to 685,711
_0585
Shewana3_0586: Shewana3_0586 - MscS mechanosensitive ion channel (RefSeq), at 685,727 to 688,930
_0586
Position (kb) 684
685 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 683.944 kb on - strand, within Shewana3_0583 at 683.986 kb on - strand, within Shewana3_0583 at 684.083 kb on - strand, within Shewana3_0583 at 684.083 kb on - strand, within Shewana3_0583 at 684.255 kb on + strand, within Shewana3_0583 at 684.263 kb on - strand, within Shewana3_0583 at 684.455 kb on + strand at 684.455 kb on + strand at 684.455 kb on + strand at 684.463 kb on - strand at 684.463 kb on - strand at 684.463 kb on - strand at 684.463 kb on - strand at 684.772 kb on - strand, within Shewana3_0584 at 684.772 kb on - strand, within Shewana3_0584 at 684.789 kb on - strand, within Shewana3_0584 at 684.813 kb on + strand, within Shewana3_0584 at 684.968 kb on - strand at 685.085 kb on - strand, within Shewana3_0585 at 685.087 kb on + strand, within Shewana3_0585 at 685.093 kb on - strand, within Shewana3_0585 at 685.130 kb on - strand, within Shewana3_0585 at 685.143 kb on + strand, within Shewana3_0585 at 685.201 kb on + strand, within Shewana3_0585 at 685.401 kb on - strand, within Shewana3_0585 at 685.606 kb on - strand, within Shewana3_0585 at 685.628 kb on + strand, within Shewana3_0585 at 685.649 kb on - strand at 685.879 kb on + strand at 685.889 kb on + strand at 685.950 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 683,944 - Shewana3_0583 0.36 -0.6 683,986 - Shewana3_0583 0.41 -2.5 684,083 - Shewana3_0583 0.51 -0.6 684,083 - Shewana3_0583 0.51 +0.2 684,255 + Shewana3_0583 0.69 -1.5 684,263 - Shewana3_0583 0.70 -0.4 684,455 + -0.2 684,455 + +0.6 684,455 + -1.0 684,463 - -1.4 684,463 - +0.3 684,463 - -0.4 684,463 - -0.3 684,772 - Shewana3_0584 0.42 -2.0 684,772 - Shewana3_0584 0.42 -0.1 684,789 - Shewana3_0584 0.47 -1.7 684,813 + Shewana3_0584 0.54 +1.2 684,968 - -0.6 685,085 - Shewana3_0585 0.13 -0.3 685,087 + Shewana3_0585 0.14 -0.4 685,093 - Shewana3_0585 0.14 +0.1 685,130 - Shewana3_0585 0.20 +0.3 685,143 + Shewana3_0585 0.21 -0.4 685,201 + Shewana3_0585 0.29 -0.1 685,401 - Shewana3_0585 0.57 -0.0 685,606 - Shewana3_0585 0.85 -1.0 685,628 + Shewana3_0585 0.88 -0.5 685,649 - -0.5 685,879 + -2.9 685,889 + -2.2 685,950 + +0.3
Or see this region's nucleotide sequence