Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0309 and Shewana3_0310 are separated by 29 nucleotides Shewana3_0310 and rbn are separated by 81 nucleotides rbn and Shewana3_0312 are separated by 96 nucleotides
Shewana3_0309: Shewana3_0309 - hypothetical protein (RefSeq), at 348,288 to 348,941
_0309
Shewana3_0310: Shewana3_0310 - hypothetical protein (RefSeq), at 348,971 to 349,192
_0310
Shewana3_0311: rbn - ribonuclease BN (RefSeq), at 349,274 to 350,287
rbn
Shewana3_0312: Shewana3_0312 - prolyl aminopeptidase (RefSeq), at 350,384 to 351,289
_0312
Shewana3_0313: Shewana3_0313 - hypothetical protein (RefSeq), at 351,286 to 351,888
_0313
Position (kb) 349
350
351 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 348.293 kb on - strand at 348.505 kb on + strand, within Shewana3_0309 at 348.505 kb on + strand, within Shewana3_0309 at 348.506 kb on - strand, within Shewana3_0309 at 348.741 kb on - strand, within Shewana3_0309 at 348.746 kb on + strand, within Shewana3_0309 at 348.917 kb on + strand at 348.925 kb on - strand at 348.925 kb on - strand at 348.925 kb on - strand at 348.986 kb on - strand at 349.005 kb on + strand, within Shewana3_0310 at 349.057 kb on + strand, within Shewana3_0310 at 349.057 kb on + strand, within Shewana3_0310 at 349.210 kb on - strand at 349.330 kb on - strand at 349.406 kb on + strand, within rbn at 349.406 kb on + strand, within rbn at 349.414 kb on - strand, within rbn at 349.427 kb on - strand, within rbn at 349.543 kb on - strand, within rbn at 349.543 kb on - strand, within rbn at 349.781 kb on - strand, within rbn at 349.867 kb on + strand, within rbn at 349.942 kb on - strand, within rbn at 349.970 kb on - strand, within rbn at 350.033 kb on - strand, within rbn at 350.100 kb on - strand, within rbn at 350.173 kb on + strand, within rbn at 350.173 kb on + strand, within rbn at 350.384 kb on + strand at 350.444 kb on - strand at 350.444 kb on - strand at 350.675 kb on + strand, within Shewana3_0312 at 350.721 kb on + strand, within Shewana3_0312 at 350.933 kb on + strand, within Shewana3_0312 at 350.983 kb on + strand, within Shewana3_0312
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 348,293 - +0.1 348,505 + Shewana3_0309 0.33 -0.6 348,505 + Shewana3_0309 0.33 -0.8 348,506 - Shewana3_0309 0.33 -0.3 348,741 - Shewana3_0309 0.69 +0.2 348,746 + Shewana3_0309 0.70 -1.8 348,917 + -1.4 348,925 - -0.5 348,925 - +0.6 348,925 - -0.4 348,986 - -0.1 349,005 + Shewana3_0310 0.15 -0.9 349,057 + Shewana3_0310 0.39 -1.1 349,057 + Shewana3_0310 0.39 -2.0 349,210 - -0.4 349,330 - -0.6 349,406 + rbn Shewana3_0311 0.13 +0.2 349,406 + rbn Shewana3_0311 0.13 +0.0 349,414 - rbn Shewana3_0311 0.14 +0.4 349,427 - rbn Shewana3_0311 0.15 -0.6 349,543 - rbn Shewana3_0311 0.27 +1.8 349,543 - rbn Shewana3_0311 0.27 -0.6 349,781 - rbn Shewana3_0311 0.50 -0.2 349,867 + rbn Shewana3_0311 0.58 +0.4 349,942 - rbn Shewana3_0311 0.66 -0.1 349,970 - rbn Shewana3_0311 0.69 -0.3 350,033 - rbn Shewana3_0311 0.75 -1.2 350,100 - rbn Shewana3_0311 0.81 +0.3 350,173 + rbn Shewana3_0311 0.89 +0.2 350,173 + rbn Shewana3_0311 0.89 -0.7 350,384 + -2.8 350,444 - -0.4 350,444 - +0.7 350,675 + Shewana3_0312 0.32 -0.1 350,721 + Shewana3_0312 0.37 +0.7 350,933 + Shewana3_0312 0.61 -0.0 350,983 + Shewana3_0312 0.66 +0.3
Or see this region's nucleotide sequence