Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0234 and Shewana3_0235 are separated by 11 nucleotides Shewana3_0235 and Shewana3_0236 overlap by 4 nucleotides Shewana3_0236 and Shewana3_0237 overlap by 4 nucleotides
Shewana3_0234: Shewana3_0234 - cytochrome c-type biogenesis protein CcmF (RefSeq), at 263,235 to 265,214
_0234
Shewana3_0235: Shewana3_0235 - periplasmic protein thiol--disulphide oxidoreductase DsbE (RefSeq), at 265,226 to 265,780
_0235
Shewana3_0236: Shewana3_0236 - cytochrome C biogenesis protein (RefSeq), at 265,777 to 266,256
_0236
Shewana3_0237: Shewana3_0237 - redoxin domain-containing protein (RefSeq), at 266,253 to 266,834
_0237
Position (kb) 265
266
267 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 264.829 kb on + strand, within Shewana3_0234 at 264.829 kb on + strand, within Shewana3_0234 at 264.874 kb on - strand, within Shewana3_0234 at 264.874 kb on - strand, within Shewana3_0234 at 265.112 kb on + strand at 265.395 kb on - strand, within Shewana3_0235 at 265.532 kb on - strand, within Shewana3_0235 at 265.562 kb on + strand, within Shewana3_0235 at 265.613 kb on + strand, within Shewana3_0235 at 265.765 kb on + strand at 265.842 kb on + strand, within Shewana3_0236 at 265.851 kb on + strand, within Shewana3_0236 at 265.915 kb on - strand, within Shewana3_0236 at 265.952 kb on + strand, within Shewana3_0236 at 266.018 kb on - strand, within Shewana3_0236 at 266.113 kb on + strand, within Shewana3_0236 at 266.113 kb on + strand, within Shewana3_0236 at 266.249 kb on - strand at 266.279 kb on - strand at 266.526 kb on + strand, within Shewana3_0237 at 266.546 kb on + strand, within Shewana3_0237 at 266.568 kb on + strand, within Shewana3_0237 at 266.624 kb on - strand, within Shewana3_0237 at 266.671 kb on + strand, within Shewana3_0237 at 266.674 kb on - strand, within Shewana3_0237 at 266.738 kb on - strand, within Shewana3_0237 at 266.800 kb on - strand at 267.077 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 264,829 + Shewana3_0234 0.81 -4.0 264,829 + Shewana3_0234 0.81 -1.3 264,874 - Shewana3_0234 0.83 -0.2 264,874 - Shewana3_0234 0.83 -1.5 265,112 + -0.6 265,395 - Shewana3_0235 0.30 -2.1 265,532 - Shewana3_0235 0.55 -2.1 265,562 + Shewana3_0235 0.61 -2.8 265,613 + Shewana3_0235 0.70 -4.0 265,765 + -0.3 265,842 + Shewana3_0236 0.14 -2.8 265,851 + Shewana3_0236 0.15 -3.9 265,915 - Shewana3_0236 0.29 +1.8 265,952 + Shewana3_0236 0.36 -2.2 266,018 - Shewana3_0236 0.50 -1.2 266,113 + Shewana3_0236 0.70 -2.8 266,113 + Shewana3_0236 0.70 -0.2 266,249 - -0.6 266,279 - -0.3 266,526 + Shewana3_0237 0.47 +0.2 266,546 + Shewana3_0237 0.50 +0.4 266,568 + Shewana3_0237 0.54 +0.2 266,624 - Shewana3_0237 0.64 -0.2 266,671 + Shewana3_0237 0.72 +0.6 266,674 - Shewana3_0237 0.72 -0.8 266,738 - Shewana3_0237 0.83 -0.1 266,800 - -0.7 267,077 + +0.2
Or see this region's nucleotide sequence