Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ribB and Shewana3_0135 are separated by 684 nucleotides Shewana3_0135 and Shewana3_0136 are separated by 22 nucleotides Shewana3_0136 and Shewana3_0137 are separated by 318 nucleotides
Shewana3_0134: ribB - 3,4-dihydroxy-2-butanone 4-phosphate synthase (RefSeq), at 152,062 to 152,715
ribB
Shewana3_0135: Shewana3_0135 - oligopeptidase B (RefSeq), at 153,400 to 155,535
_0135
Shewana3_0136: Shewana3_0136 - excinuclease ABC subunit C (RefSeq), at 155,558 to 155,980
_0136
Shewana3_0137: Shewana3_0137 - hypothetical protein (RefSeq), at 156,299 to 157,129
_0137
Position (kb) 153
154
155
156 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 152.486 kb on - strand, within ribB at 152.707 kb on - strand at 152.792 kb on + strand at 152.825 kb on + strand at 152.864 kb on + strand at 152.872 kb on - strand at 152.955 kb on - strand at 153.015 kb on + strand at 153.271 kb on + strand at 153.271 kb on + strand at 153.279 kb on - strand at 153.279 kb on - strand at 153.279 kb on - strand at 153.279 kb on - strand at 153.326 kb on - strand at 153.337 kb on + strand at 153.638 kb on - strand, within Shewana3_0135 at 153.643 kb on - strand, within Shewana3_0135 at 153.806 kb on + strand, within Shewana3_0135 at 153.876 kb on - strand, within Shewana3_0135 at 154.060 kb on + strand, within Shewana3_0135 at 154.082 kb on + strand, within Shewana3_0135 at 154.090 kb on - strand, within Shewana3_0135 at 154.097 kb on + strand, within Shewana3_0135 at 154.105 kb on + strand, within Shewana3_0135 at 154.157 kb on - strand, within Shewana3_0135 at 154.229 kb on + strand, within Shewana3_0135 at 154.229 kb on + strand, within Shewana3_0135 at 154.237 kb on - strand, within Shewana3_0135 at 154.249 kb on + strand, within Shewana3_0135 at 154.291 kb on - strand, within Shewana3_0135 at 154.291 kb on - strand, within Shewana3_0135 at 154.485 kb on + strand, within Shewana3_0135 at 154.493 kb on - strand, within Shewana3_0135 at 154.529 kb on + strand, within Shewana3_0135 at 154.529 kb on + strand, within Shewana3_0135 at 154.617 kb on - strand, within Shewana3_0135 at 154.617 kb on - strand, within Shewana3_0135 at 154.759 kb on - strand, within Shewana3_0135 at 154.898 kb on + strand, within Shewana3_0135 at 155.017 kb on + strand, within Shewana3_0135 at 155.025 kb on - strand, within Shewana3_0135 at 155.031 kb on + strand, within Shewana3_0135 at 155.158 kb on - strand, within Shewana3_0135 at 155.251 kb on + strand, within Shewana3_0135 at 155.350 kb on - strand at 155.507 kb on + strand at 155.515 kb on - strand at 155.761 kb on + strand, within Shewana3_0136 at 155.761 kb on + strand, within Shewana3_0136 at 155.769 kb on - strand, within Shewana3_0136 at 155.769 kb on - strand, within Shewana3_0136 at 155.796 kb on + strand, within Shewana3_0136 at 155.829 kb on - strand, within Shewana3_0136 at 155.908 kb on - strand, within Shewana3_0136 at 156.069 kb on + strand at 156.126 kb on - strand at 156.316 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 152,486 - ribB Shewana3_0134 0.65 -0.1 152,707 - -0.1 152,792 + -0.8 152,825 + -0.1 152,864 + +1.2 152,872 - -0.5 152,955 - -0.1 153,015 + -0.5 153,271 + +0.4 153,271 + +0.5 153,279 - -0.2 153,279 - +0.6 153,279 - +0.6 153,279 - +0.7 153,326 - +0.2 153,337 + +0.7 153,638 - Shewana3_0135 0.11 +0.4 153,643 - Shewana3_0135 0.11 -0.1 153,806 + Shewana3_0135 0.19 -2.6 153,876 - Shewana3_0135 0.22 -0.3 154,060 + Shewana3_0135 0.31 -0.0 154,082 + Shewana3_0135 0.32 -0.3 154,090 - Shewana3_0135 0.32 -0.9 154,097 + Shewana3_0135 0.33 -0.0 154,105 + Shewana3_0135 0.33 -0.6 154,157 - Shewana3_0135 0.35 -0.6 154,229 + Shewana3_0135 0.39 -1.8 154,229 + Shewana3_0135 0.39 -0.2 154,237 - Shewana3_0135 0.39 -0.3 154,249 + Shewana3_0135 0.40 -0.2 154,291 - Shewana3_0135 0.42 -1.1 154,291 - Shewana3_0135 0.42 -0.6 154,485 + Shewana3_0135 0.51 +0.2 154,493 - Shewana3_0135 0.51 -0.8 154,529 + Shewana3_0135 0.53 +0.1 154,529 + Shewana3_0135 0.53 +0.6 154,617 - Shewana3_0135 0.57 -1.0 154,617 - Shewana3_0135 0.57 -0.7 154,759 - Shewana3_0135 0.64 -1.7 154,898 + Shewana3_0135 0.70 +0.0 155,017 + Shewana3_0135 0.76 -0.2 155,025 - Shewana3_0135 0.76 -0.6 155,031 + Shewana3_0135 0.76 +0.2 155,158 - Shewana3_0135 0.82 -0.9 155,251 + Shewana3_0135 0.87 +0.3 155,350 - +0.3 155,507 + +0.7 155,515 - -0.3 155,761 + Shewana3_0136 0.48 -0.4 155,761 + Shewana3_0136 0.48 +0.7 155,769 - Shewana3_0136 0.50 +0.1 155,769 - Shewana3_0136 0.50 -0.4 155,796 + Shewana3_0136 0.56 -1.2 155,829 - Shewana3_0136 0.64 -0.1 155,908 - Shewana3_0136 0.83 +0.2 156,069 + -0.1 156,126 - -3.6 156,316 - -0.0
Or see this region's nucleotide sequence