Experiment: Chloramphenicol 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0020 and Shewana3_0021 are separated by 35 nucleotides Shewana3_0021 and Shewana3_0022 are separated by 152 nucleotides Shewana3_0022 and fadA are separated by 224 nucleotides
Shewana3_0020: Shewana3_0020 - MOSC domain-containing protein (RefSeq), at 25,944 to 26,381
_0020
Shewana3_0021: Shewana3_0021 - sulfur transfer protein SirA (RefSeq), at 26,417 to 26,662
_0021
Shewana3_0022: Shewana3_0022 - diguanylate cyclase (RefSeq), at 26,815 to 28,026
_0022
Shewana3_0023: fadA - Acetyl-CoA C-acyltransferase (EC 2.3.1.16) <small>(from data)</small>, at 28,251 to 29,414
fadA
Position (kb) 26
27
28
29 Strain fitness (log2 ratio) -2
-1
0
1 at 25.854 kb on - strand at 25.896 kb on + strand at 25.912 kb on + strand at 25.919 kb on - strand at 25.929 kb on - strand at 25.955 kb on - strand at 25.955 kb on - strand at 26.087 kb on - strand, within Shewana3_0020 at 26.111 kb on - strand, within Shewana3_0020 at 26.250 kb on + strand, within Shewana3_0020 at 26.293 kb on + strand, within Shewana3_0020 at 26.301 kb on - strand, within Shewana3_0020 at 26.301 kb on - strand, within Shewana3_0020 at 26.301 kb on - strand, within Shewana3_0020 at 26.398 kb on - strand at 26.757 kb on + strand at 26.765 kb on - strand at 26.804 kb on + strand at 26.882 kb on + strand at 26.890 kb on - strand at 26.957 kb on - strand, within Shewana3_0022 at 27.167 kb on + strand, within Shewana3_0022 at 27.167 kb on + strand, within Shewana3_0022 at 27.175 kb on - strand, within Shewana3_0022 at 27.242 kb on + strand, within Shewana3_0022 at 27.279 kb on - strand, within Shewana3_0022 at 27.314 kb on - strand, within Shewana3_0022 at 27.315 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.348 kb on - strand, within Shewana3_0022 at 27.381 kb on + strand, within Shewana3_0022 at 27.438 kb on + strand, within Shewana3_0022 at 27.446 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.763 kb on - strand, within Shewana3_0022 at 27.831 kb on + strand, within Shewana3_0022 at 27.850 kb on + strand, within Shewana3_0022 at 27.978 kb on + strand at 28.041 kb on - strand at 28.069 kb on + strand at 28.069 kb on + strand at 28.069 kb on + strand at 28.071 kb on + strand at 28.077 kb on - strand at 28.077 kb on - strand at 28.077 kb on - strand at 28.143 kb on - strand at 28.169 kb on + strand at 28.249 kb on + strand at 28.256 kb on - strand at 28.283 kb on + strand at 28.283 kb on + strand at 28.292 kb on - strand at 28.375 kb on - strand, within fadA at 28.377 kb on + strand, within fadA at 28.385 kb on - strand, within fadA at 28.418 kb on - strand, within fadA at 28.428 kb on - strand, within fadA at 28.438 kb on - strand, within fadA at 28.445 kb on + strand, within fadA at 28.445 kb on + strand, within fadA at 28.454 kb on + strand, within fadA at 28.464 kb on + strand, within fadA at 28.473 kb on - strand, within fadA at 28.473 kb on - strand, within fadA at 28.473 kb on - strand, within fadA at 28.734 kb on + strand, within fadA at 28.742 kb on - strand, within fadA at 28.804 kb on + strand, within fadA at 28.841 kb on - strand, within fadA at 28.874 kb on - strand, within fadA at 28.903 kb on - strand, within fadA at 28.931 kb on + strand, within fadA at 28.947 kb on - strand, within fadA at 29.012 kb on - strand, within fadA
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.0001 mg/ml remove 25,854 - -0.6 25,896 + -0.6 25,912 + -0.2 25,919 - -0.4 25,929 - -0.2 25,955 - -0.1 25,955 - -0.3 26,087 - Shewana3_0020 0.33 +0.0 26,111 - Shewana3_0020 0.38 +0.4 26,250 + Shewana3_0020 0.70 -0.2 26,293 + Shewana3_0020 0.80 -0.8 26,301 - Shewana3_0020 0.82 -0.5 26,301 - Shewana3_0020 0.82 -1.3 26,301 - Shewana3_0020 0.82 -0.2 26,398 - -0.2 26,757 + -0.5 26,765 - -2.7 26,804 + -0.1 26,882 + +0.4 26,890 - -2.4 26,957 - Shewana3_0022 0.12 -1.9 27,167 + Shewana3_0022 0.29 -0.2 27,167 + Shewana3_0022 0.29 -0.3 27,175 - Shewana3_0022 0.30 -0.5 27,242 + Shewana3_0022 0.35 -0.6 27,279 - Shewana3_0022 0.38 -1.2 27,314 - Shewana3_0022 0.41 -1.1 27,315 + Shewana3_0022 0.41 -0.1 27,340 + Shewana3_0022 0.43 -0.2 27,340 + Shewana3_0022 0.43 -0.5 27,340 + Shewana3_0022 0.43 +0.8 27,348 - Shewana3_0022 0.44 -2.1 27,381 + Shewana3_0022 0.47 -0.2 27,438 + Shewana3_0022 0.51 +0.2 27,446 - Shewana3_0022 0.52 -1.1 27,763 - Shewana3_0022 0.78 -0.7 27,763 - Shewana3_0022 0.78 +0.2 27,763 - Shewana3_0022 0.78 -1.8 27,763 - Shewana3_0022 0.78 -0.2 27,831 + Shewana3_0022 0.84 +0.2 27,850 + Shewana3_0022 0.85 +0.1 27,978 + +0.3 28,041 - -0.8 28,069 + -0.4 28,069 + -0.3 28,069 + +0.2 28,071 + -0.1 28,077 - -0.1 28,077 - -0.5 28,077 - -0.1 28,143 - -0.7 28,169 + -0.2 28,249 + -1.1 28,256 - -0.3 28,283 + -0.4 28,283 + -0.1 28,292 - +0.4 28,375 - fadA Shewana3_0023 0.11 +0.0 28,377 + fadA Shewana3_0023 0.11 -0.2 28,385 - fadA Shewana3_0023 0.12 +0.6 28,418 - fadA Shewana3_0023 0.14 +0.4 28,428 - fadA Shewana3_0023 0.15 +0.3 28,438 - fadA Shewana3_0023 0.16 +1.0 28,445 + fadA Shewana3_0023 0.17 +0.5 28,445 + fadA Shewana3_0023 0.17 -0.8 28,454 + fadA Shewana3_0023 0.17 -0.1 28,464 + fadA Shewana3_0023 0.18 -0.7 28,473 - fadA Shewana3_0023 0.19 +0.0 28,473 - fadA Shewana3_0023 0.19 +0.4 28,473 - fadA Shewana3_0023 0.19 +0.7 28,734 + fadA Shewana3_0023 0.41 +0.2 28,742 - fadA Shewana3_0023 0.42 +0.2 28,804 + fadA Shewana3_0023 0.48 -0.5 28,841 - fadA Shewana3_0023 0.51 +1.1 28,874 - fadA Shewana3_0023 0.54 +0.4 28,903 - fadA Shewana3_0023 0.56 -0.1 28,931 + fadA Shewana3_0023 0.58 -0.3 28,947 - fadA Shewana3_0023 0.60 +0.3 29,012 - fadA Shewana3_0023 0.65 +0.2
Or see this region's nucleotide sequence