Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1158

Experiment: acetate 80 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1156 and Pf6N2E2_1157 are separated by 39 nucleotidesPf6N2E2_1157 and Pf6N2E2_1158 are separated by 92 nucleotidesPf6N2E2_1158 and Pf6N2E2_1159 are separated by 250 nucleotides Pf6N2E2_1156 - hypothetical protein, at 1,353,678 to 1,354,211 _1156 Pf6N2E2_1157 - hypothetical protein possibly connected to lactam utilization and allophanate hydrolase, at 1,354,251 to 1,355,042 _1157 Pf6N2E2_1158 - Cyn operon transcriptional activator, at 1,355,135 to 1,356,082 _1158 Pf6N2E2_1159 - Sialic acid transporter (permease) NanT, at 1,356,333 to 1,357,628 _1159 Position (kb) 1355 1356 1357Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1354.180 kb on + strandat 1354.180 kb on + strandat 1354.180 kb on + strandat 1354.181 kb on - strandat 1354.253 kb on - strandat 1354.270 kb on - strandat 1354.350 kb on + strand, within Pf6N2E2_1157at 1354.350 kb on + strand, within Pf6N2E2_1157at 1354.408 kb on + strand, within Pf6N2E2_1157at 1354.461 kb on + strand, within Pf6N2E2_1157at 1354.762 kb on + strand, within Pf6N2E2_1157at 1354.779 kb on + strand, within Pf6N2E2_1157at 1354.780 kb on - strand, within Pf6N2E2_1157at 1354.973 kb on - strandat 1355.109 kb on + strandat 1355.109 kb on + strandat 1355.130 kb on - strandat 1355.463 kb on + strand, within Pf6N2E2_1158at 1355.463 kb on + strand, within Pf6N2E2_1158at 1355.464 kb on - strand, within Pf6N2E2_1158at 1355.464 kb on - strand, within Pf6N2E2_1158at 1355.464 kb on - strand, within Pf6N2E2_1158at 1355.477 kb on + strand, within Pf6N2E2_1158at 1355.478 kb on - strand, within Pf6N2E2_1158at 1355.551 kb on - strand, within Pf6N2E2_1158at 1355.562 kb on + strand, within Pf6N2E2_1158at 1355.562 kb on + strand, within Pf6N2E2_1158at 1355.563 kb on - strand, within Pf6N2E2_1158at 1355.789 kb on - strand, within Pf6N2E2_1158at 1355.937 kb on - strand, within Pf6N2E2_1158at 1356.038 kb on + strandat 1356.110 kb on + strandat 1356.110 kb on + strandat 1356.110 kb on + strandat 1356.111 kb on - strandat 1356.111 kb on - strandat 1356.111 kb on - strandat 1356.111 kb on - strandat 1356.171 kb on + strandat 1356.425 kb on + strandat 1356.425 kb on + strandat 1356.425 kb on + strandat 1356.425 kb on + strandat 1356.687 kb on + strand, within Pf6N2E2_1159at 1356.687 kb on + strand, within Pf6N2E2_1159at 1356.687 kb on + strand, within Pf6N2E2_1159at 1356.687 kb on + strand, within Pf6N2E2_1159at 1356.855 kb on + strand, within Pf6N2E2_1159at 1356.856 kb on - strand, within Pf6N2E2_1159

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 80 mM
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1,354,180 + +0.9
1,354,180 + +0.2
1,354,180 + +0.2
1,354,181 - +0.6
1,354,253 - -0.2
1,354,270 - -0.3
1,354,350 + Pf6N2E2_1157 0.12 +0.4
1,354,350 + Pf6N2E2_1157 0.12 -0.5
1,354,408 + Pf6N2E2_1157 0.20 -0.3
1,354,461 + Pf6N2E2_1157 0.27 +0.5
1,354,762 + Pf6N2E2_1157 0.65 -0.8
1,354,779 + Pf6N2E2_1157 0.67 -0.1
1,354,780 - Pf6N2E2_1157 0.67 +0.2
1,354,973 - -0.3
1,355,109 + +0.4
1,355,109 + -0.4
1,355,130 - +2.3
1,355,463 + Pf6N2E2_1158 0.35 -0.3
1,355,463 + Pf6N2E2_1158 0.35 +0.3
1,355,464 - Pf6N2E2_1158 0.35 -0.8
1,355,464 - Pf6N2E2_1158 0.35 +0.4
1,355,464 - Pf6N2E2_1158 0.35 +0.4
1,355,477 + Pf6N2E2_1158 0.36 -0.6
1,355,478 - Pf6N2E2_1158 0.36 -0.0
1,355,551 - Pf6N2E2_1158 0.44 +0.3
1,355,562 + Pf6N2E2_1158 0.45 -0.2
1,355,562 + Pf6N2E2_1158 0.45 -2.2
1,355,563 - Pf6N2E2_1158 0.45 +0.5
1,355,789 - Pf6N2E2_1158 0.69 -0.3
1,355,937 - Pf6N2E2_1158 0.85 -0.5
1,356,038 + -0.5
1,356,110 + +0.2
1,356,110 + +1.1
1,356,110 + -0.2
1,356,111 - -3.2
1,356,111 - +1.1
1,356,111 - -0.1
1,356,111 - +0.7
1,356,171 + +0.2
1,356,425 + -0.1
1,356,425 + -0.0
1,356,425 + -0.7
1,356,425 + +0.5
1,356,687 + Pf6N2E2_1159 0.27 +0.4
1,356,687 + Pf6N2E2_1159 0.27 +0.5
1,356,687 + Pf6N2E2_1159 0.27 +0.3
1,356,687 + Pf6N2E2_1159 0.27 -0.4
1,356,855 + Pf6N2E2_1159 0.40 +0.2
1,356,856 - Pf6N2E2_1159 0.40 +0.2

Or see this region's nucleotide sequence