Experiment: acetate 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1151 and Pf6N2E2_1152 are separated by 94 nucleotides Pf6N2E2_1152 and Pf6N2E2_1153 are separated by 3 nucleotides Pf6N2E2_1153 and Pf6N2E2_1154 are separated by 77 nucleotides Pf6N2E2_1154 and Pf6N2E2_1155 are separated by 294 nucleotides
Pf6N2E2_1151 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 1,348,712 to 1,350,091
_1151
Pf6N2E2_1152 - hypothetical protein, at 1,350,186 to 1,350,920
_1152
Pf6N2E2_1153 - small molecule metabolism; degradation; fatty acid, at 1,350,924 to 1,351,739
_1153
Pf6N2E2_1154 - FIG00978082: hypothetical protein, at 1,351,817 to 1,352,404
_1154
Pf6N2E2_1155 - Cyn operon transcriptional activator, at 1,352,699 to 1,353,706
_1155
Position (kb)
1350
1351
1352 Strain fitness (log2 ratio)
-2
-1
0
1 at 1349.952 kb on + strand, within Pf6N2E2_1151 at 1349.952 kb on + strand, within Pf6N2E2_1151 at 1349.952 kb on + strand, within Pf6N2E2_1151 at 1350.060 kb on - strand at 1350.060 kb on - strand at 1350.060 kb on - strand at 1350.064 kb on - strand at 1350.064 kb on - strand at 1350.166 kb on + strand at 1350.191 kb on + strand at 1350.192 kb on - strand at 1350.230 kb on - strand at 1350.230 kb on - strand at 1350.406 kb on + strand, within Pf6N2E2_1152 at 1350.407 kb on - strand, within Pf6N2E2_1152 at 1350.667 kb on + strand, within Pf6N2E2_1152 at 1350.709 kb on + strand, within Pf6N2E2_1152 at 1350.709 kb on + strand, within Pf6N2E2_1152 at 1350.710 kb on - strand, within Pf6N2E2_1152 at 1350.871 kb on + strand at 1350.871 kb on + strand at 1350.871 kb on + strand at 1350.871 kb on + strand at 1350.872 kb on - strand at 1350.925 kb on + strand at 1350.925 kb on + strand at 1350.925 kb on + strand at 1350.925 kb on + strand at 1351.181 kb on - strand, within Pf6N2E2_1153 at 1351.270 kb on + strand, within Pf6N2E2_1153 at 1351.270 kb on + strand, within Pf6N2E2_1153 at 1351.271 kb on - strand, within Pf6N2E2_1153 at 1351.355 kb on - strand, within Pf6N2E2_1153 at 1351.447 kb on + strand, within Pf6N2E2_1153 at 1351.507 kb on + strand, within Pf6N2E2_1153 at 1351.514 kb on - strand, within Pf6N2E2_1153 at 1351.718 kb on + strand at 1351.743 kb on + strand at 1351.743 kb on + strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.744 kb on - strand at 1351.747 kb on + strand at 1351.748 kb on - strand at 1351.815 kb on - strand at 1351.904 kb on + strand, within Pf6N2E2_1154 at 1351.905 kb on - strand, within Pf6N2E2_1154 at 1351.957 kb on - strand, within Pf6N2E2_1154 at 1351.957 kb on - strand, within Pf6N2E2_1154 at 1351.957 kb on - strand, within Pf6N2E2_1154 at 1352.001 kb on + strand, within Pf6N2E2_1154 at 1352.001 kb on + strand, within Pf6N2E2_1154 at 1352.001 kb on + strand, within Pf6N2E2_1154 at 1352.002 kb on - strand, within Pf6N2E2_1154 at 1352.052 kb on + strand, within Pf6N2E2_1154 at 1352.053 kb on - strand, within Pf6N2E2_1154 at 1352.122 kb on - strand, within Pf6N2E2_1154 at 1352.122 kb on - strand, within Pf6N2E2_1154 at 1352.122 kb on - strand, within Pf6N2E2_1154 at 1352.293 kb on + strand, within Pf6N2E2_1154 at 1352.294 kb on - strand, within Pf6N2E2_1154 at 1352.495 kb on + strand at 1352.495 kb on + strand at 1352.495 kb on + strand at 1352.495 kb on + strand at 1352.496 kb on - strand at 1352.496 kb on - strand at 1352.496 kb on - strand at 1352.496 kb on - strand at 1352.564 kb on - strand at 1352.655 kb on + strand at 1352.655 kb on + strand at 1352.655 kb on + strand at 1352.655 kb on + strand at 1352.656 kb on - strand at 1352.656 kb on - strand at 1352.656 kb on - strand at 1352.672 kb on + strand at 1352.672 kb on + strand at 1352.672 kb on + strand at 1352.672 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 80 mM remove 1,349,952 + Pf6N2E2_1151 0.90 -0.3 1,349,952 + Pf6N2E2_1151 0.90 -0.1 1,349,952 + Pf6N2E2_1151 0.90 +0.1 1,350,060 - -0.8 1,350,060 - -0.5 1,350,060 - +0.4 1,350,064 - +0.4 1,350,064 - +0.1 1,350,166 + +0.8 1,350,191 + +0.3 1,350,192 - -0.0 1,350,230 - -0.4 1,350,230 - +0.7 1,350,406 + Pf6N2E2_1152 0.30 +0.1 1,350,407 - Pf6N2E2_1152 0.30 +0.5 1,350,667 + Pf6N2E2_1152 0.65 +0.1 1,350,709 + Pf6N2E2_1152 0.71 -0.4 1,350,709 + Pf6N2E2_1152 0.71 +0.3 1,350,710 - Pf6N2E2_1152 0.71 +0.3 1,350,871 + -0.3 1,350,871 + -0.6 1,350,871 + +0.0 1,350,871 + +0.5 1,350,872 - -0.1 1,350,925 + -0.4 1,350,925 + -0.0 1,350,925 + -0.1 1,350,925 + -0.2 1,351,181 - Pf6N2E2_1153 0.31 -0.0 1,351,270 + Pf6N2E2_1153 0.42 -1.7 1,351,270 + Pf6N2E2_1153 0.42 -1.5 1,351,271 - Pf6N2E2_1153 0.43 -0.3 1,351,355 - Pf6N2E2_1153 0.53 -0.7 1,351,447 + Pf6N2E2_1153 0.64 +0.3 1,351,507 + Pf6N2E2_1153 0.71 -0.3 1,351,514 - Pf6N2E2_1153 0.72 -0.0 1,351,718 + -0.2 1,351,743 + +0.7 1,351,743 + +0.2 1,351,744 - -0.2 1,351,744 - +0.1 1,351,744 - +0.1 1,351,744 - +0.6 1,351,744 - -0.3 1,351,747 + -0.4 1,351,748 - +0.7 1,351,815 - -0.8 1,351,904 + Pf6N2E2_1154 0.15 -0.2 1,351,905 - Pf6N2E2_1154 0.15 -1.1 1,351,957 - Pf6N2E2_1154 0.24 -1.3 1,351,957 - Pf6N2E2_1154 0.24 -0.4 1,351,957 - Pf6N2E2_1154 0.24 +0.5 1,352,001 + Pf6N2E2_1154 0.31 -0.3 1,352,001 + Pf6N2E2_1154 0.31 +0.4 1,352,001 + Pf6N2E2_1154 0.31 -0.2 1,352,002 - Pf6N2E2_1154 0.31 +0.1 1,352,052 + Pf6N2E2_1154 0.40 -1.4 1,352,053 - Pf6N2E2_1154 0.40 +0.2 1,352,122 - Pf6N2E2_1154 0.52 -0.5 1,352,122 - Pf6N2E2_1154 0.52 -0.6 1,352,122 - Pf6N2E2_1154 0.52 +1.2 1,352,293 + Pf6N2E2_1154 0.81 +0.5 1,352,294 - Pf6N2E2_1154 0.81 -0.1 1,352,495 + +0.2 1,352,495 + +0.2 1,352,495 + +0.3 1,352,495 + -0.0 1,352,496 - +0.2 1,352,496 - +0.5 1,352,496 - -0.1 1,352,496 - +0.7 1,352,564 - -0.3 1,352,655 + -0.4 1,352,655 + -0.2 1,352,655 + -0.2 1,352,655 + -2.7 1,352,656 - -0.0 1,352,656 - +0.7 1,352,656 - +0.4 1,352,672 + -0.2 1,352,672 + +0.5 1,352,672 + +1.0 1,352,672 + +0.3
Or see this region's nucleotide sequence