Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS16655

Experiment: Growth at pH8 and (C) Trisodium citrate

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Growth at pH8 and (C) Trisodium citrate
3,775,569	+		PFLU_RS16650	0.26	-1.1
3,775,569	+		PFLU_RS16650	0.26	+0.5
3,775,575	+		PFLU_RS16650	0.26	+2.2
3,775,576	-		PFLU_RS16650	0.26	+2.8
3,775,639	-		PFLU_RS16650	0.31	-2.5
3,775,677	+		PFLU_RS16650	0.34	+3.1
3,775,678	-		PFLU_RS16650	0.34	-1.0
3,775,678	-		PFLU_RS16650	0.34	+3.4
3,775,686	-		PFLU_RS16650	0.35	-0.8
3,775,686	-		PFLU_RS16650	0.35	+0.8
3,775,707	+		PFLU_RS16650	0.36	+0.3
3,775,707	+		PFLU_RS16650	0.36	+2.6
3,775,707	+		PFLU_RS16650	0.36	+0.5
3,775,707	+		PFLU_RS16650	0.36	-1.2
3,775,707	+		PFLU_RS16650	0.36	+2.5
3,775,708	-		PFLU_RS16650	0.37	-0.8
3,775,708	-		PFLU_RS16650	0.37	+3.4
3,775,708	-		PFLU_RS16650	0.37	-0.2
3,775,708	-		PFLU_RS16650	0.37	+1.5
3,775,711	-		PFLU_RS16650	0.37	+0.8
3,775,736	+		PFLU_RS16650	0.39	-1.8
3,775,736	+		PFLU_RS16650	0.39	-2.2
3,775,736	+		PFLU_RS16650	0.39	-1.1
3,775,736	+		PFLU_RS16650	0.39	-0.7
3,775,737	-		PFLU_RS16650	0.39	-0.2
3,775,737	-		PFLU_RS16650	0.39	+2.5
3,775,815	+		PFLU_RS16650	0.45	-1.0
3,775,815	+		PFLU_RS16650	0.45	-0.7
3,775,815	+		PFLU_RS16650	0.45	-1.2
3,775,816	-		PFLU_RS16650	0.45	-0.2
3,775,816	-		PFLU_RS16650	0.45	+0.4
3,775,836	+		PFLU_RS16650	0.47	-0.7
3,775,836	+		PFLU_RS16650	0.47	+2.2
3,775,907	+		PFLU_RS16650	0.52	-0.8
3,775,907	+		PFLU_RS16650	0.52	-0.5
3,775,907	+		PFLU_RS16650	0.52	+0.8
3,775,908	-		PFLU_RS16650	0.52	+1.6
3,775,908	-		PFLU_RS16650	0.52	+1.2
3,775,908	-		PFLU_RS16650	0.52	+0.6
3,775,974	+		PFLU_RS16650	0.57	-1.8
3,775,975	-		PFLU_RS16650	0.57	+2.7
3,775,975	-		PFLU_RS16650	0.57	+0.1
3,775,975	-		PFLU_RS16650	0.57	-1.0
3,775,975	-		PFLU_RS16650	0.57	+1.5
3,776,046	+		PFLU_RS16650	0.63	+0.6
3,776,046	+		PFLU_RS16650	0.63	+0.3
3,776,046	+		PFLU_RS16650	0.63	+1.7
3,776,046	+		PFLU_RS16650	0.63	-0.1
3,776,047	-		PFLU_RS16650	0.63	+1.1
3,776,047	-		PFLU_RS16650	0.63	-2.8
3,776,047	-		PFLU_RS16650	0.63	-0.9
3,776,047	-		PFLU_RS16650	0.63	-2.3
3,776,056	+		PFLU_RS16650	0.64	-0.4
3,776,101	+		PFLU_RS16650	0.67	+2.8
3,776,101	+		PFLU_RS16650	0.67	-0.9
3,776,137	-		PFLU_RS16650	0.70	-1.8
3,776,182	+		PFLU_RS16650	0.74	-3.2
3,776,211	+		PFLU_RS16650	0.76	-1.2
3,776,212	-		PFLU_RS16650	0.76	+1.2
3,776,212	-		PFLU_RS16650	0.76	-1.8
3,776,249	+		PFLU_RS16650	0.79	-0.2
3,776,249	+		PFLU_RS16650	0.79	-0.5
3,776,262	-		PFLU_RS16650	0.80	-0.5
3,776,287	+		PFLU_RS16650	0.82	-0.5
3,776,288	-		PFLU_RS16650	0.82	-2.0
3,776,288	-		PFLU_RS16650	0.82	+1.7
3,776,300	+		PFLU_RS16650	0.83	-0.2
3,776,300	+		PFLU_RS16650	0.83	+3.0
3,776,362	-		PFLU_RS16650	0.88	-0.5
3,776,473	+				+2.4
3,776,474	-				+1.8
3,776,506	-				+1.5
3,776,509	+				-0.4
3,776,509	+				-1.5
3,776,509	+				-2.4
3,776,509	+				+0.2
3,776,510	-				+1.6
3,776,510	-				-1.7
3,776,574	+				+0.5
3,776,574	+				-1.0
3,776,575	-				+1.8
3,776,628	-				+1.2
3,776,630	-				+0.8
3,776,668	-		PFLU_RS16655	0.13	-1.2
3,776,709	-		PFLU_RS16655	0.18	+0.8
3,776,759	+		PFLU_RS16655	0.24	-1.7
3,776,760	-		PFLU_RS16655	0.24	+1.7
3,776,782	+		PFLU_RS16655	0.27	-0.5
3,776,803	-		PFLU_RS16655	0.29	-1.4
3,776,839	-		PFLU_RS16655	0.33	+0.5
3,776,843	+		PFLU_RS16655	0.34	-0.2
3,777,011	-		PFLU_RS16655	0.54	+1.5
3,777,011	-		PFLU_RS16655	0.54	-1.8
3,777,011	-		PFLU_RS16655	0.54	-0.2
3,777,055	+		PFLU_RS16655	0.59	-1.9
3,777,064	-		PFLU_RS16655	0.60	+0.2
3,777,134	+		PFLU_RS16655	0.69	+0.2
3,777,139	+		PFLU_RS16655	0.69	-0.7
3,777,192	+		PFLU_RS16655	0.76	-2.5
3,777,193	-		PFLU_RS16655	0.76	-1.4
3,777,197	-		PFLU_RS16655	0.76	+0.2
3,777,241	-		PFLU_RS16655	0.82	-1.5
3,777,286	-		PFLU_RS16655	0.87	-2.1
3,777,290	+		PFLU_RS16655	0.88	+4.7
3,777,366	+				+0.5
3,777,619	+		PFLU_RS16660	0.24	-0.2
3,777,619	+		PFLU_RS16660	0.24	-0.9
3,777,736	+		PFLU_RS16660	0.40	-2.4
3,777,737	-		PFLU_RS16660	0.41	+3.7
3,777,757	-		PFLU_RS16660	0.43	-1.4
3,777,863	+		PFLU_RS16660	0.58	+2.5
3,777,867	-		PFLU_RS16660	0.59	-0.9
3,777,867	-		PFLU_RS16660	0.59	-0.2
3,777,867	-		PFLU_RS16660	0.59	-2.0
3,777,871	+		PFLU_RS16660	0.59	+0.2
3,777,937	-		PFLU_RS16660	0.68	-2.0
3,777,937	-		PFLU_RS16660	0.68	+2.5
3,777,960	+		PFLU_RS16660	0.71	-1.2
3,778,004	-		PFLU_RS16660	0.77	-1.9
3,778,004	-		PFLU_RS16660	0.77	+1.5
3,778,073	+		PFLU_RS16660	0.87	+0.6
3,778,073	+		PFLU_RS16660	0.87	+0.4
3,778,073	+		PFLU_RS16660	0.87	+0.2
3,778,074	-		PFLU_RS16660	0.87	-1.2
3,778,074	-		PFLU_RS16660	0.87	-0.5
3,778,074	-		PFLU_RS16660	0.87	-0.2
3,778,074	-		PFLU_RS16660	0.87	-0.5
3,778,074	-		PFLU_RS16660	0.87	+0.8
3,778,074	-		PFLU_RS16660	0.87	-1.2
3,778,089	+		PFLU_RS16660	0.89	+2.6
3,778,092	+		PFLU_RS16660	0.90	+1.5
3,778,147	+				+1.8
3,778,148	-				+3.4
3,778,148	-				-2.0
3,778,165	+				+1.5
3,778,165	+				+0.0
3,778,165	+				+2.3
3,778,165	+				+1.0
3,778,166	-				-1.5
3,778,166	-				+2.0
3,778,166	-				-0.1
3,778,171	+				-2.4
3,778,171	+				-0.8
3,778,190	+				-0.5
3,778,190	+				+0.5
3,778,190	+				-2.1
3,778,190	+				-0.0
3,778,190	+				-1.0
3,778,190	+				+0.2
3,778,190	+				+0.5
3,778,190	+				-1.0
3,778,190	+				+0.3
3,778,191	-				-1.0
3,778,191	-				-0.5
3,778,191	-				+0.2
3,778,191	-				-0.8
3,778,191	-				-1.2
3,778,191	-				+3.9
3,778,191	-				+0.8
3,778,194	+				-1.2
3,778,194	+				-2.6
3,778,194	+				-0.2
3,778,194	+				-1.4
3,778,194	+				-0.2
3,778,194	+				-1.6
3,778,194	+				+3.1
3,778,194	+				+0.6
3,778,194	+				+0.2
3,778,194	+				-1.4
3,778,195	-				-1.4
3,778,195	-				+0.8
3,778,195	-				+1.4
3,778,195	-				-1.6
3,778,195	-				-2.8
3,778,196	+				-1.0
3,778,196	+				-1.0
3,778,196	+				-0.5
3,778,197	-				+1.5
3,778,197	-				+4.4
3,778,197	-				+3.2
3,778,197	-				+5.2
3,778,209	+				-0.5
3,778,304	+		PFLU_RS16665	0.14	-1.6
3,778,330	+		PFLU_RS16665	0.19	-2.4
3,778,330	+		PFLU_RS16665	0.19	-1.4
3,778,330	+		PFLU_RS16665	0.19	-0.5
3,778,331	-		PFLU_RS16665	0.19	+1.1
3,778,331	-		PFLU_RS16665	0.19	-2.4