Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05140 and H281DRAFT_05141 are separated by 2 nucleotides H281DRAFT_05141 and H281DRAFT_05142 are separated by 58 nucleotides H281DRAFT_05142 and H281DRAFT_05143 are separated by 149 nucleotides H281DRAFT_05143 and H281DRAFT_05144 are separated by 6 nucleotides
H281DRAFT_05140: H281DRAFT_05140 - Thiosulfate reductase cytochrome b subunit, at 94,350 to 94,997
_05140
H281DRAFT_05141: H281DRAFT_05141 - sulfoxide reductase catalytic subunit YedY, at 95,000 to 95,791
_05141
H281DRAFT_05142: H281DRAFT_05142 - hypothetical protein, at 95,850 to 96,464
_05142
H281DRAFT_05143: H281DRAFT_05143 - Predicted membrane protein, at 96,614 to 97,456
_05143
H281DRAFT_05144: H281DRAFT_05144 - amino acid ABC transporter substrate-binding protein, PAAT family, at 97,463 to 98,197
_05144
Position (kb) 95
96
97 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 95.310 kb on - strand, within H281DRAFT_05141 at 95.310 kb on - strand, within H281DRAFT_05141 at 95.310 kb on - strand, within H281DRAFT_05141 at 95.315 kb on - strand, within H281DRAFT_05141 at 95.443 kb on + strand, within H281DRAFT_05141 at 95.443 kb on + strand, within H281DRAFT_05141 at 95.538 kb on - strand, within H281DRAFT_05141 at 95.565 kb on - strand, within H281DRAFT_05141 at 95.651 kb on + strand, within H281DRAFT_05141 at 95.652 kb on - strand, within H281DRAFT_05141 at 95.739 kb on - strand at 95.769 kb on - strand at 95.846 kb on - strand at 96.187 kb on - strand, within H281DRAFT_05142 at 96.187 kb on - strand, within H281DRAFT_05142 at 96.279 kb on + strand, within H281DRAFT_05142 at 96.280 kb on - strand, within H281DRAFT_05142 at 96.319 kb on + strand, within H281DRAFT_05142 at 96.320 kb on - strand, within H281DRAFT_05142 at 96.323 kb on + strand, within H281DRAFT_05142 at 96.323 kb on + strand, within H281DRAFT_05142 at 96.323 kb on + strand, within H281DRAFT_05142 at 96.323 kb on + strand, within H281DRAFT_05142 at 96.411 kb on + strand at 96.411 kb on + strand at 96.412 kb on - strand at 96.463 kb on - strand at 96.779 kb on + strand, within H281DRAFT_05143 at 96.939 kb on + strand, within H281DRAFT_05143 at 96.993 kb on + strand, within H281DRAFT_05143 at 96.993 kb on + strand, within H281DRAFT_05143 at 96.994 kb on - strand, within H281DRAFT_05143 at 97.219 kb on - strand, within H281DRAFT_05143 at 97.273 kb on + strand, within H281DRAFT_05143 at 97.273 kb on + strand, within H281DRAFT_05143 at 97.274 kb on - strand, within H281DRAFT_05143 at 97.274 kb on - strand, within H281DRAFT_05143 at 97.275 kb on + strand, within H281DRAFT_05143 at 97.275 kb on + strand, within H281DRAFT_05143 at 97.446 kb on + strand at 97.446 kb on + strand at 97.447 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 95,310 - H281DRAFT_05141 0.39 +0.0 95,310 - H281DRAFT_05141 0.39 -0.7 95,310 - H281DRAFT_05141 0.39 +0.4 95,315 - H281DRAFT_05141 0.40 +0.4 95,443 + H281DRAFT_05141 0.56 -3.7 95,443 + H281DRAFT_05141 0.56 +0.3 95,538 - H281DRAFT_05141 0.68 -0.1 95,565 - H281DRAFT_05141 0.71 +0.3 95,651 + H281DRAFT_05141 0.82 +0.1 95,652 - H281DRAFT_05141 0.82 +1.7 95,739 - -0.1 95,769 - +0.4 95,846 - +1.1 96,187 - H281DRAFT_05142 0.55 +0.3 96,187 - H281DRAFT_05142 0.55 -0.1 96,279 + H281DRAFT_05142 0.70 +0.7 96,280 - H281DRAFT_05142 0.70 +0.2 96,319 + H281DRAFT_05142 0.76 +1.1 96,320 - H281DRAFT_05142 0.76 -0.5 96,323 + H281DRAFT_05142 0.77 -0.2 96,323 + H281DRAFT_05142 0.77 -0.0 96,323 + H281DRAFT_05142 0.77 +0.3 96,323 + H281DRAFT_05142 0.77 +0.7 96,411 + -0.2 96,411 + -0.2 96,412 - -0.5 96,463 - +0.4 96,779 + H281DRAFT_05143 0.20 +0.0 96,939 + H281DRAFT_05143 0.39 -0.8 96,993 + H281DRAFT_05143 0.45 -0.9 96,993 + H281DRAFT_05143 0.45 -0.5 96,994 - H281DRAFT_05143 0.45 -0.7 97,219 - H281DRAFT_05143 0.72 -0.3 97,273 + H281DRAFT_05143 0.78 -1.0 97,273 + H281DRAFT_05143 0.78 +0.6 97,274 - H281DRAFT_05143 0.78 -0.3 97,274 - H281DRAFT_05143 0.78 +0.1 97,275 + H281DRAFT_05143 0.78 -0.0 97,275 + H281DRAFT_05143 0.78 +0.6 97,446 + +0.1 97,446 + +0.2 97,447 - +0.6
Or see this region's nucleotide sequence