Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04978 and H281DRAFT_04979 are separated by 48 nucleotides H281DRAFT_04979 and H281DRAFT_04980 are separated by 228 nucleotides H281DRAFT_04980 and H281DRAFT_04981 are separated by 221 nucleotides
H281DRAFT_04978: H281DRAFT_04978 - 23S rRNA (adenine2030-N6)-methyltransferase, at 162,840 to 163,904
_04978
H281DRAFT_04979: H281DRAFT_04979 - NitT/TauT family transport system substrate-binding protein, at 163,953 to 164,990
_04979
H281DRAFT_04980: H281DRAFT_04980 - hypothetical protein, at 165,219 to 165,596
_04980
H281DRAFT_04981: H281DRAFT_04981 - Protein of unknown function (DUF3563), at 165,818 to 165,964
_04981
Position (kb) 163
164
165 Strain fitness (log2 ratio) -2
-1
0
1 at 163.132 kb on + strand, within H281DRAFT_04978 at 163.132 kb on + strand, within H281DRAFT_04978 at 163.133 kb on - strand, within H281DRAFT_04978 at 163.140 kb on + strand, within H281DRAFT_04978 at 163.170 kb on + strand, within H281DRAFT_04978 at 163.170 kb on + strand, within H281DRAFT_04978 at 163.171 kb on - strand, within H281DRAFT_04978 at 163.200 kb on + strand, within H281DRAFT_04978 at 163.219 kb on - strand, within H281DRAFT_04978 at 163.300 kb on - strand, within H281DRAFT_04978 at 163.362 kb on + strand, within H281DRAFT_04978 at 163.398 kb on - strand, within H281DRAFT_04978 at 163.543 kb on + strand, within H281DRAFT_04978 at 163.543 kb on + strand, within H281DRAFT_04978 at 163.561 kb on - strand, within H281DRAFT_04978 at 163.632 kb on - strand, within H281DRAFT_04978 at 163.635 kb on + strand, within H281DRAFT_04978 at 163.994 kb on + strand at 163.994 kb on + strand at 163.995 kb on - strand at 163.995 kb on - strand at 163.996 kb on + strand at 163.997 kb on - strand at 163.997 kb on - strand at 164.105 kb on - strand, within H281DRAFT_04979 at 164.120 kb on + strand, within H281DRAFT_04979 at 164.137 kb on + strand, within H281DRAFT_04979 at 164.138 kb on - strand, within H281DRAFT_04979 at 164.274 kb on - strand, within H281DRAFT_04979 at 164.470 kb on - strand, within H281DRAFT_04979 at 164.470 kb on - strand, within H281DRAFT_04979 at 164.697 kb on + strand, within H281DRAFT_04979 at 164.698 kb on - strand, within H281DRAFT_04979 at 164.698 kb on - strand, within H281DRAFT_04979 at 164.698 kb on - strand, within H281DRAFT_04979 at 164.698 kb on - strand, within H281DRAFT_04979 at 164.698 kb on - strand, within H281DRAFT_04979 at 164.698 kb on - strand, within H281DRAFT_04979 at 164.698 kb on - strand, within H281DRAFT_04979 at 164.698 kb on - strand, within H281DRAFT_04979 at 164.849 kb on - strand, within H281DRAFT_04979 at 164.851 kb on + strand, within H281DRAFT_04979 at 165.008 kb on + strand at 165.008 kb on + strand at 165.009 kb on - strand at 165.009 kb on - strand at 165.009 kb on - strand at 165.195 kb on - strand at 165.266 kb on - strand, within H281DRAFT_04980 at 165.277 kb on + strand, within H281DRAFT_04980 at 165.328 kb on + strand, within H281DRAFT_04980 at 165.329 kb on - strand, within H281DRAFT_04980 at 165.329 kb on - strand, within H281DRAFT_04980 at 165.646 kb on + strand at 165.647 kb on - strand at 165.674 kb on + strand at 165.675 kb on - strand at 165.775 kb on - strand at 165.785 kb on + strand at 165.786 kb on - strand at 165.786 kb on - strand at 165.819 kb on + strand at 165.894 kb on + strand, within H281DRAFT_04981 at 165.894 kb on + strand, within H281DRAFT_04981 at 165.895 kb on - strand, within H281DRAFT_04981 at 165.895 kb on - strand, within H281DRAFT_04981 at 165.954 kb on + strand at 165.954 kb on + strand at 165.954 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 163,132 + H281DRAFT_04978 0.27 -0.0 163,132 + H281DRAFT_04978 0.27 -2.5 163,133 - H281DRAFT_04978 0.28 +0.4 163,140 + H281DRAFT_04978 0.28 -0.2 163,170 + H281DRAFT_04978 0.31 +0.6 163,170 + H281DRAFT_04978 0.31 -0.3 163,171 - H281DRAFT_04978 0.31 +0.2 163,200 + H281DRAFT_04978 0.34 -0.4 163,219 - H281DRAFT_04978 0.36 +0.9 163,300 - H281DRAFT_04978 0.43 +0.5 163,362 + H281DRAFT_04978 0.49 -0.1 163,398 - H281DRAFT_04978 0.52 +0.3 163,543 + H281DRAFT_04978 0.66 +0.1 163,543 + H281DRAFT_04978 0.66 -1.7 163,561 - H281DRAFT_04978 0.68 +0.3 163,632 - H281DRAFT_04978 0.74 +0.6 163,635 + H281DRAFT_04978 0.75 -0.4 163,994 + +0.0 163,994 + +0.6 163,995 - +0.5 163,995 - +0.3 163,996 + +0.2 163,997 - -0.2 163,997 - -0.1 164,105 - H281DRAFT_04979 0.15 -0.6 164,120 + H281DRAFT_04979 0.16 +0.7 164,137 + H281DRAFT_04979 0.18 +0.1 164,138 - H281DRAFT_04979 0.18 -0.7 164,274 - H281DRAFT_04979 0.31 +0.2 164,470 - H281DRAFT_04979 0.50 -1.9 164,470 - H281DRAFT_04979 0.50 +0.2 164,697 + H281DRAFT_04979 0.72 +0.2 164,698 - H281DRAFT_04979 0.72 -0.6 164,698 - H281DRAFT_04979 0.72 +1.2 164,698 - H281DRAFT_04979 0.72 +0.7 164,698 - H281DRAFT_04979 0.72 -0.7 164,698 - H281DRAFT_04979 0.72 +0.7 164,698 - H281DRAFT_04979 0.72 -0.2 164,698 - H281DRAFT_04979 0.72 -0.1 164,698 - H281DRAFT_04979 0.72 +0.6 164,849 - H281DRAFT_04979 0.86 -0.1 164,851 + H281DRAFT_04979 0.87 -0.2 165,008 + +0.3 165,008 + -0.5 165,009 - +0.0 165,009 - +0.6 165,009 - +0.0 165,195 - -0.2 165,266 - H281DRAFT_04980 0.12 -1.0 165,277 + H281DRAFT_04980 0.15 -0.8 165,328 + H281DRAFT_04980 0.29 +0.2 165,329 - H281DRAFT_04980 0.29 -0.3 165,329 - H281DRAFT_04980 0.29 +0.3 165,646 + +0.2 165,647 - -0.4 165,674 + +0.7 165,675 - +0.7 165,775 - -0.6 165,785 + +1.1 165,786 - -1.3 165,786 - +0.6 165,819 + -0.8 165,894 + H281DRAFT_04981 0.52 -0.1 165,894 + H281DRAFT_04981 0.52 +0.9 165,895 - H281DRAFT_04981 0.52 +0.4 165,895 - H281DRAFT_04981 0.52 +0.9 165,954 + +1.7 165,954 + -0.0 165,954 + +0.4
Or see this region's nucleotide sequence