Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04148 and H281DRAFT_04149 overlap by 14 nucleotides H281DRAFT_04149 and H281DRAFT_04150 are separated by 64 nucleotides
H281DRAFT_04148: H281DRAFT_04148 - monosaccharide ABC transporter membrane protein, CUT2 family, at 258,389 to 259,396
_04148
H281DRAFT_04149: H281DRAFT_04149 - monosaccharide ABC transporter ATP-binding protein, CUT2 family, at 259,383 to 260,903
_04149
H281DRAFT_04150: H281DRAFT_04150 - monosaccharide ABC transporter substrate-binding protein, CUT2 family, at 260,968 to 261,921
_04150
Position (kb) 259
260
261 Strain fitness (log2 ratio) -1
0
1 at 258.390 kb on + strand at 258.397 kb on + strand at 258.415 kb on - strand at 258.688 kb on - strand, within H281DRAFT_04148 at 258.726 kb on - strand, within H281DRAFT_04148 at 259.426 kb on + strand at 259.427 kb on - strand at 260.283 kb on + strand, within H281DRAFT_04149 at 260.871 kb on - strand at 261.347 kb on - strand, within H281DRAFT_04150 at 261.461 kb on - strand, within H281DRAFT_04150 at 261.464 kb on + strand, within H281DRAFT_04150 at 261.465 kb on - strand, within H281DRAFT_04150 at 261.886 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 258,390 + +0.8 258,397 + +0.0 258,415 - +0.4 258,688 - H281DRAFT_04148 0.30 +1.3 258,726 - H281DRAFT_04148 0.33 -0.9 259,426 + +0.5 259,427 - -0.6 260,283 + H281DRAFT_04149 0.59 -0.2 260,871 - -0.2 261,347 - H281DRAFT_04150 0.40 -1.1 261,461 - H281DRAFT_04150 0.52 +0.2 261,464 + H281DRAFT_04150 0.52 -0.8 261,465 - H281DRAFT_04150 0.52 -0.0 261,886 - +0.1
Or see this region's nucleotide sequence